Effects of Enzyme Volumes on Hydrolysis and Fermentation for Ethanol Production From Leftover Cooked Rice
This study aimed to utilize the enzymatic hydrolysis of leftover cooked rice (LCR) for fermentative ethanol production. Effect of glucoamylase volumes (V1: 5 U/g, V2: 25 U/g, and V3: 50 U/g) on the performance of LCR hydrolysis was also evaluated. It was found that the highest chemical oxygen demand...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.631089/full |
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| author | Xikai Chen Xikai Chen Yujia Zhao Yujia Zhao Boyang Chen Wang Su Wang Su Zhengxian Zhang Zhengxian Zhang Yanxu Liu Xiaobin Xu Xiaobin Xu Junhong Tang Pingzhi Hou Pingzhi Hou Wei Han Wei Han Wei Han |
| author_facet | Xikai Chen Xikai Chen Yujia Zhao Yujia Zhao Boyang Chen Wang Su Wang Su Zhengxian Zhang Zhengxian Zhang Yanxu Liu Xiaobin Xu Xiaobin Xu Junhong Tang Pingzhi Hou Pingzhi Hou Wei Han Wei Han Wei Han |
| author_sort | Xikai Chen |
| collection | DOAJ |
| description | This study aimed to utilize the enzymatic hydrolysis of leftover cooked rice (LCR) for fermentative ethanol production. Effect of glucoamylase volumes (V1: 5 U/g, V2: 25 U/g, and V3: 50 U/g) on the performance of LCR hydrolysis was also evaluated. It was found that the highest chemical oxygen demand (COD) of 77.5 g/L and reducing sugar (RS) of 34.6 g/L were achieved at V3. The LCR hydrolyzate obtained from enzymatic hydrolysis was then used as feedstock for ethanol fermentation. Higher ethanol production was obtained when RS increased from 18.7 g/L (V1) to 23.2 g/L (V2). However, lower ethanol production was found when RS further increased to 34.6 g/L (V3) probably because too high RS concentration led to the inhibition on the yeast. The maximum ethanol production and yield were 21.1 g/L and 0.3 g ethanol/g LCR, respectively. The LCR could be a promising substrate for fermentative ethanol production for industrial application. |
| first_indexed | 2024-12-22T11:28:33Z |
| format | Article |
| id | doaj.art-ad3b95d199de463f80ad0bd8876e17de |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-22T11:28:33Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-ad3b95d199de463f80ad0bd8876e17de2022-12-21T18:27:41ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-05-01910.3389/fbioe.2021.631089631089Effects of Enzyme Volumes on Hydrolysis and Fermentation for Ethanol Production From Leftover Cooked RiceXikai Chen0Xikai Chen1Yujia Zhao2Yujia Zhao3Boyang Chen4Wang Su5Wang Su6Zhengxian Zhang7Zhengxian Zhang8Yanxu Liu9Xiaobin Xu10Xiaobin Xu11Junhong Tang12Pingzhi Hou13Pingzhi Hou14Wei Han15Wei Han16Wei Han17College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, ChinaZhejiang Jinhuanbao Biotechnology Company Limited, Huzhou, ChinaCollege of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, ChinaZhejiang Jinhuanbao Biotechnology Company Limited, Huzhou, ChinaSchool of Material Science and Engineering, Shanghai Jiao Tong University, Shanghai, ChinaZhejiang Jinhuanbao Biotechnology Company Limited, Huzhou, ChinaCollege of Electronics and Information, Hangzhou Dianzi University, Hangzhou, ChinaZhejiang Jinhuanbao Biotechnology Company Limited, Huzhou, ChinaCollege of Electronics and Information, Hangzhou Dianzi University, Hangzhou, ChinaCollege of Environmental and Chemical Engineering, Shanghai University, Shanghai, ChinaSchool of Automation, The Belt and Road Information Research Institute, Hangzhou Dianzi University, Hangzhou, ChinaThe Sci-Tech Academic Institute, Hangzhou Dianzi University, Hangzhou, ChinaCollege of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, ChinaSchool of Automation, The Belt and Road Information Research Institute, Hangzhou Dianzi University, Hangzhou, ChinaThe Sci-Tech Academic Institute, Hangzhou Dianzi University, Hangzhou, ChinaCollege of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, ChinaZhejiang Jinhuanbao Biotechnology Company Limited, Huzhou, ChinaSchool of Automation, The Belt and Road Information Research Institute, Hangzhou Dianzi University, Hangzhou, ChinaThis study aimed to utilize the enzymatic hydrolysis of leftover cooked rice (LCR) for fermentative ethanol production. Effect of glucoamylase volumes (V1: 5 U/g, V2: 25 U/g, and V3: 50 U/g) on the performance of LCR hydrolysis was also evaluated. It was found that the highest chemical oxygen demand (COD) of 77.5 g/L and reducing sugar (RS) of 34.6 g/L were achieved at V3. The LCR hydrolyzate obtained from enzymatic hydrolysis was then used as feedstock for ethanol fermentation. Higher ethanol production was obtained when RS increased from 18.7 g/L (V1) to 23.2 g/L (V2). However, lower ethanol production was found when RS further increased to 34.6 g/L (V3) probably because too high RS concentration led to the inhibition on the yeast. The maximum ethanol production and yield were 21.1 g/L and 0.3 g ethanol/g LCR, respectively. The LCR could be a promising substrate for fermentative ethanol production for industrial application.https://www.frontiersin.org/articles/10.3389/fbioe.2021.631089/fullethanol fermentationglucoamylaseleftover cooked ricereducing sugarhydrolysis |
| spellingShingle | Xikai Chen Xikai Chen Yujia Zhao Yujia Zhao Boyang Chen Wang Su Wang Su Zhengxian Zhang Zhengxian Zhang Yanxu Liu Xiaobin Xu Xiaobin Xu Junhong Tang Pingzhi Hou Pingzhi Hou Wei Han Wei Han Wei Han Effects of Enzyme Volumes on Hydrolysis and Fermentation for Ethanol Production From Leftover Cooked Rice Frontiers in Bioengineering and Biotechnology ethanol fermentation glucoamylase leftover cooked rice reducing sugar hydrolysis |
| title | Effects of Enzyme Volumes on Hydrolysis and Fermentation for Ethanol Production From Leftover Cooked Rice |
| title_full | Effects of Enzyme Volumes on Hydrolysis and Fermentation for Ethanol Production From Leftover Cooked Rice |
| title_fullStr | Effects of Enzyme Volumes on Hydrolysis and Fermentation for Ethanol Production From Leftover Cooked Rice |
| title_full_unstemmed | Effects of Enzyme Volumes on Hydrolysis and Fermentation for Ethanol Production From Leftover Cooked Rice |
| title_short | Effects of Enzyme Volumes on Hydrolysis and Fermentation for Ethanol Production From Leftover Cooked Rice |
| title_sort | effects of enzyme volumes on hydrolysis and fermentation for ethanol production from leftover cooked rice |
| topic | ethanol fermentation glucoamylase leftover cooked rice reducing sugar hydrolysis |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.631089/full |
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