Antenatal Vitamin A Decreases Ventilation-induced Lung Injury in the Lamb Model of Congenital Diaphragmatic Hernia
Infants with congenital diaphragmatic hernia (CDH) are susceptible to ventilation-induced lung injury. Vitamin A may protect the lung from injury during ventilation. The authors investigated the effects of antenatal vitamin A on ventilation-induced lung injury in CDH lambs using lung myeloperoxidase...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2006-07-01
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| Series: | Asian Journal of Surgery |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1015958409600865 |
| _version_ | 1828351965317300224 |
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| author | Nicola A. Lewis Bruce A. Holm Daniel Swartz June Sokolowski Jon Rossman Philip L. Glick |
| author_facet | Nicola A. Lewis Bruce A. Holm Daniel Swartz June Sokolowski Jon Rossman Philip L. Glick |
| author_sort | Nicola A. Lewis |
| collection | DOAJ |
| description | Infants with congenital diaphragmatic hernia (CDH) are susceptible to ventilation-induced lung injury. Vitamin A may protect the lung from injury during ventilation. The authors investigated the effects of antenatal vitamin A on ventilation-induced lung injury in CDH lambs using lung myeloperoxidase (MPO) activity as an indicator of lung injury.
Methods: Left-sided diaphragmatic defects were created in 10 lambs at 79-81 days' gestation. Six CDH lambs had right jugular venous catheters inserted at 120 days' gestation and were given vitamin A until 135 days' gestation. Four CDH lambs were not treated. Twin littermates (n = 3) served as controls. All lambs were delivered at 136-139 days of gestation and ventilated for 2 hours. Lambs were sacrificed following ventilation and samples of left lung were snap frozen. MPO was extracted from lung tissue and MPO activity was assayed.
Results: CDH lambs treated with antenatal vitamin A demonstrated significantly lower MPO activity than untreated CDH lambs (0.0477 ± 0.0150 vs. 0.1106 ± 0.0230 units/mg protein, p < 0.05).
Conclusion: This is the first study to look at the effect of vitamin A on lung injury in CDH. In the lamb model of CDH, antenatal vitamin A decreases ventilation-induced lung injury. |
| first_indexed | 2024-04-14T01:47:12Z |
| format | Article |
| id | doaj.art-ad400cfdd61949c6b685e9fb90e0d26f |
| institution | Directory Open Access Journal |
| issn | 1015-9584 |
| language | English |
| last_indexed | 2024-04-14T01:47:12Z |
| publishDate | 2006-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Asian Journal of Surgery |
| spelling | doaj.art-ad400cfdd61949c6b685e9fb90e0d26f2022-12-22T02:19:30ZengElsevierAsian Journal of Surgery1015-95842006-07-0129319319710.1016/S1015-9584(09)60086-5Antenatal Vitamin A Decreases Ventilation-induced Lung Injury in the Lamb Model of Congenital Diaphragmatic HerniaNicola A. LewisBruce A. HolmDaniel SwartzJune SokolowskiJon RossmanPhilip L. GlickInfants with congenital diaphragmatic hernia (CDH) are susceptible to ventilation-induced lung injury. Vitamin A may protect the lung from injury during ventilation. The authors investigated the effects of antenatal vitamin A on ventilation-induced lung injury in CDH lambs using lung myeloperoxidase (MPO) activity as an indicator of lung injury. Methods: Left-sided diaphragmatic defects were created in 10 lambs at 79-81 days' gestation. Six CDH lambs had right jugular venous catheters inserted at 120 days' gestation and were given vitamin A until 135 days' gestation. Four CDH lambs were not treated. Twin littermates (n = 3) served as controls. All lambs were delivered at 136-139 days of gestation and ventilated for 2 hours. Lambs were sacrificed following ventilation and samples of left lung were snap frozen. MPO was extracted from lung tissue and MPO activity was assayed. Results: CDH lambs treated with antenatal vitamin A demonstrated significantly lower MPO activity than untreated CDH lambs (0.0477 ± 0.0150 vs. 0.1106 ± 0.0230 units/mg protein, p < 0.05). Conclusion: This is the first study to look at the effect of vitamin A on lung injury in CDH. In the lamb model of CDH, antenatal vitamin A decreases ventilation-induced lung injury.http://www.sciencedirect.com/science/article/pii/S1015958409600865CDHcongenital diaphragmatic hernialung injurymyeloperoxidase activityventilation-induced lung injuryvitamin A |
| spellingShingle | Nicola A. Lewis Bruce A. Holm Daniel Swartz June Sokolowski Jon Rossman Philip L. Glick Antenatal Vitamin A Decreases Ventilation-induced Lung Injury in the Lamb Model of Congenital Diaphragmatic Hernia Asian Journal of Surgery CDH congenital diaphragmatic hernia lung injury myeloperoxidase activity ventilation-induced lung injury vitamin A |
| title | Antenatal Vitamin A Decreases Ventilation-induced Lung Injury in the Lamb Model of Congenital Diaphragmatic Hernia |
| title_full | Antenatal Vitamin A Decreases Ventilation-induced Lung Injury in the Lamb Model of Congenital Diaphragmatic Hernia |
| title_fullStr | Antenatal Vitamin A Decreases Ventilation-induced Lung Injury in the Lamb Model of Congenital Diaphragmatic Hernia |
| title_full_unstemmed | Antenatal Vitamin A Decreases Ventilation-induced Lung Injury in the Lamb Model of Congenital Diaphragmatic Hernia |
| title_short | Antenatal Vitamin A Decreases Ventilation-induced Lung Injury in the Lamb Model of Congenital Diaphragmatic Hernia |
| title_sort | antenatal vitamin a decreases ventilation induced lung injury in the lamb model of congenital diaphragmatic hernia |
| topic | CDH congenital diaphragmatic hernia lung injury myeloperoxidase activity ventilation-induced lung injury vitamin A |
| url | http://www.sciencedirect.com/science/article/pii/S1015958409600865 |
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