Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis
The sensitivity and specificity of clinical diagnostic indicators and non-invasive diagnostic methods for endometriosis at early stage is not optimal. Previous studies demonstrated that abnormal lipid metabolism was involved in the pathological development of endometriosis. Our cross-sectional study...
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2018.00014/full |
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| author | Jingjie Li Yue Gao Lihuan Guan Huizhen Zhang Jiahong Sun Xiao Gong Dongshun Li Pan Chen Zheng Ma Xiaoyan Liang Min Huang Huichang Bi |
| author_facet | Jingjie Li Yue Gao Lihuan Guan Huizhen Zhang Jiahong Sun Xiao Gong Dongshun Li Pan Chen Zheng Ma Xiaoyan Liang Min Huang Huichang Bi |
| author_sort | Jingjie Li |
| collection | DOAJ |
| description | The sensitivity and specificity of clinical diagnostic indicators and non-invasive diagnostic methods for endometriosis at early stage is not optimal. Previous studies demonstrated that abnormal lipid metabolism was involved in the pathological development of endometriosis. Our cross-sectional study included 21 patients with laparoscopically confirmed endometriosis at stage I–II and 20 infertile women who underwent diagnostic laparoscopy combined with hysteroscopy from January 2014 to January 2015. Eutopic endometrium was collected by pipelle endometrial biopsy. Lipid metabolites were quantified by ultra-high performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (UHPLC-ESI-HRMS). Lipid profiles of endometriosis patients at early stage (I–II) was characterized by a decreased concentration of phosphatidylcholine (18:1/22:6), (20:1/14:1), (20:3/20:4), and phosphatidylserine (20:3/23:1) and an increased concentration of phosphatidic acid (25:5/22:6) compared with control. The synthesized predicting strategy with 5 biomarkers has a specificity of 75.0% and a sensitivity of 90.5%. Lipid profile of eutopic endometrium in endometriosis was effectively characterized by UHPLC-ESI-HRMS-based metabolomics. Our study demonstrated the alteration of phosphatidic acid, phosphatidylcholine, phosphatidylserine metabolites in endometriosis and provided potential biomarkers for semi-invasive diagnose of endometriosis at early stage. |
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| publishDate | 2018-01-01 |
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| series | Frontiers in Physiology |
| spelling | doaj.art-ad42a3ffb0814b2b936ca38a76bc19212022-12-21T20:06:19ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-01-01910.3389/fphys.2018.00014330926Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of EndometriosisJingjie Li0Yue Gao1Lihuan Guan2Huizhen Zhang3Jiahong Sun4Xiao Gong5Dongshun Li6Pan Chen7Zheng Ma8Xiaoyan Liang9Min Huang10Huichang Bi11Center of Reproductive Medicine, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaSchool of Public Health, Guangdong Pharmaceutical University, Guangzhou, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaDepartment of Pharmacy, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaInstitute of Population Research, Peking University, Beijing, ChinaCenter of Reproductive Medicine, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaThe sensitivity and specificity of clinical diagnostic indicators and non-invasive diagnostic methods for endometriosis at early stage is not optimal. Previous studies demonstrated that abnormal lipid metabolism was involved in the pathological development of endometriosis. Our cross-sectional study included 21 patients with laparoscopically confirmed endometriosis at stage I–II and 20 infertile women who underwent diagnostic laparoscopy combined with hysteroscopy from January 2014 to January 2015. Eutopic endometrium was collected by pipelle endometrial biopsy. Lipid metabolites were quantified by ultra-high performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (UHPLC-ESI-HRMS). Lipid profiles of endometriosis patients at early stage (I–II) was characterized by a decreased concentration of phosphatidylcholine (18:1/22:6), (20:1/14:1), (20:3/20:4), and phosphatidylserine (20:3/23:1) and an increased concentration of phosphatidic acid (25:5/22:6) compared with control. The synthesized predicting strategy with 5 biomarkers has a specificity of 75.0% and a sensitivity of 90.5%. Lipid profile of eutopic endometrium in endometriosis was effectively characterized by UHPLC-ESI-HRMS-based metabolomics. Our study demonstrated the alteration of phosphatidic acid, phosphatidylcholine, phosphatidylserine metabolites in endometriosis and provided potential biomarkers for semi-invasive diagnose of endometriosis at early stage.http://journal.frontiersin.org/article/10.3389/fphys.2018.00014/fullendometriosislipid profilingUHPLC-ESI-HRMSeutopic endometriumearly diagnosis |
| spellingShingle | Jingjie Li Yue Gao Lihuan Guan Huizhen Zhang Jiahong Sun Xiao Gong Dongshun Li Pan Chen Zheng Ma Xiaoyan Liang Min Huang Huichang Bi Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis Frontiers in Physiology endometriosis lipid profiling UHPLC-ESI-HRMS eutopic endometrium early diagnosis |
| title | Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis |
| title_full | Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis |
| title_fullStr | Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis |
| title_full_unstemmed | Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis |
| title_short | Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis |
| title_sort | discovery of phosphatidic acid phosphatidylcholine and phosphatidylserine as biomarkers for early diagnosis of endometriosis |
| topic | endometriosis lipid profiling UHPLC-ESI-HRMS eutopic endometrium early diagnosis |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2018.00014/full |
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