Fibrous Hydrogels for Cell Encapsulation: A Modular and Supramolecular Approach.
Artificial 3-dimensional (3D) cell culture systems, which mimic the extracellular matrix (ECM), hold great potential as models to study cellular processes under controlled conditions. The natural ECM is a 3D structure composed of a fibrous hydrogel that provides both mechanical and biochemical cues...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155625&type=printable |
| _version_ | 1826584060815736832 |
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| author | Małgorzata K Włodarczyk-Biegun Kambiz Farbod Marc W T Werten Cornelis J Slingerland Frits A de Wolf Jeroen J J P van den Beucken Sander C G Leeuwenburgh Martien A Cohen Stuart Marleen Kamperman |
| author_facet | Małgorzata K Włodarczyk-Biegun Kambiz Farbod Marc W T Werten Cornelis J Slingerland Frits A de Wolf Jeroen J J P van den Beucken Sander C G Leeuwenburgh Martien A Cohen Stuart Marleen Kamperman |
| author_sort | Małgorzata K Włodarczyk-Biegun |
| collection | DOAJ |
| description | Artificial 3-dimensional (3D) cell culture systems, which mimic the extracellular matrix (ECM), hold great potential as models to study cellular processes under controlled conditions. The natural ECM is a 3D structure composed of a fibrous hydrogel that provides both mechanical and biochemical cues to instruct cell behavior. Here we present an ECM-mimicking genetically engineered protein-based hydrogel as a 3D cell culture system that combines several key features: (1) Mild and straightforward encapsulation meters (1) ease of ut I am not so sure.encapsulation of the cells, without the need of an external crosslinker. (2) Supramolecular assembly resulting in a fibrous architecture that recapitulates some of the unique mechanical characteristics of the ECM, i.e. strain-stiffening and self-healing behavior. (3) A modular approach allowing controlled incorporation of the biochemical cue density (integrin binding RGD domains). We tested the gels by encapsulating MG-63 osteoblastic cells and found that encapsulated cells not only respond to higher RGD density, but also to overall gel concentration. Cells in 1% and 2% (weight fraction) protein gels showed spreading and proliferation, provided a relative RGD density of at least 50%. In contrast, in 4% gels very little spreading and proliferation occurred, even for a relative RGD density of 100%. The independent control over both mechanical and biochemical cues obtained in this modular approach renders our hydrogels suitable to study cellular responses under highly defined conditions. |
| first_indexed | 2024-12-12T15:44:03Z |
| format | Article |
| id | doaj.art-ad46d24b9be74a4c91d186f62e916ed6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T15:31:45Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ad46d24b9be74a4c91d186f62e916ed62025-02-25T05:35:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015562510.1371/journal.pone.0155625Fibrous Hydrogels for Cell Encapsulation: A Modular and Supramolecular Approach.Małgorzata K Włodarczyk-BiegunKambiz FarbodMarc W T WertenCornelis J SlingerlandFrits A de WolfJeroen J J P van den BeuckenSander C G LeeuwenburghMartien A Cohen StuartMarleen KampermanArtificial 3-dimensional (3D) cell culture systems, which mimic the extracellular matrix (ECM), hold great potential as models to study cellular processes under controlled conditions. The natural ECM is a 3D structure composed of a fibrous hydrogel that provides both mechanical and biochemical cues to instruct cell behavior. Here we present an ECM-mimicking genetically engineered protein-based hydrogel as a 3D cell culture system that combines several key features: (1) Mild and straightforward encapsulation meters (1) ease of ut I am not so sure.encapsulation of the cells, without the need of an external crosslinker. (2) Supramolecular assembly resulting in a fibrous architecture that recapitulates some of the unique mechanical characteristics of the ECM, i.e. strain-stiffening and self-healing behavior. (3) A modular approach allowing controlled incorporation of the biochemical cue density (integrin binding RGD domains). We tested the gels by encapsulating MG-63 osteoblastic cells and found that encapsulated cells not only respond to higher RGD density, but also to overall gel concentration. Cells in 1% and 2% (weight fraction) protein gels showed spreading and proliferation, provided a relative RGD density of at least 50%. In contrast, in 4% gels very little spreading and proliferation occurred, even for a relative RGD density of 100%. The independent control over both mechanical and biochemical cues obtained in this modular approach renders our hydrogels suitable to study cellular responses under highly defined conditions.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155625&type=printable |
| spellingShingle | Małgorzata K Włodarczyk-Biegun Kambiz Farbod Marc W T Werten Cornelis J Slingerland Frits A de Wolf Jeroen J J P van den Beucken Sander C G Leeuwenburgh Martien A Cohen Stuart Marleen Kamperman Fibrous Hydrogels for Cell Encapsulation: A Modular and Supramolecular Approach. PLoS ONE |
| title | Fibrous Hydrogels for Cell Encapsulation: A Modular and Supramolecular Approach. |
| title_full | Fibrous Hydrogels for Cell Encapsulation: A Modular and Supramolecular Approach. |
| title_fullStr | Fibrous Hydrogels for Cell Encapsulation: A Modular and Supramolecular Approach. |
| title_full_unstemmed | Fibrous Hydrogels for Cell Encapsulation: A Modular and Supramolecular Approach. |
| title_short | Fibrous Hydrogels for Cell Encapsulation: A Modular and Supramolecular Approach. |
| title_sort | fibrous hydrogels for cell encapsulation a modular and supramolecular approach |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155625&type=printable |
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