A Comparison of the Oral Microbiota in Healthy Dogs and Dogs with Oral Tumors
The aim of this study was to further describe the oral microbiota of healthy dogs by DNA shotgun sequencing and compare those to dogs with oral tumors. Oral swabs (representative of all niches of the oral cavity) were collected from healthy dogs (n = 24) and from dogs with different oral tumors (n =...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/23/3594 |
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| author | Anja Lisjak Bruna Correa Lopes Rachel Pilla Ana Nemec Jan S. Suchodolski Nataša Tozon |
| author_facet | Anja Lisjak Bruna Correa Lopes Rachel Pilla Ana Nemec Jan S. Suchodolski Nataša Tozon |
| author_sort | Anja Lisjak |
| collection | DOAJ |
| description | The aim of this study was to further describe the oral microbiota of healthy dogs by DNA shotgun sequencing and compare those to dogs with oral tumors. Oral swabs (representative of all niches of the oral cavity) were collected from healthy dogs (n = 24) and from dogs with different oral tumors (n = 7). DNA was extracted from the swabs and shotgun metagenomic sequencing was performed. Only minor differences in microbiota composition were observed between the two groups. At the phylum level, the Bacteroidota, Proteobacteria, Actinobacteriota, Desulfobacterota and Firmicutes were most abundant in both groups. Observed Operational Taxonomic Units—OTUs (species richness) was significantly higher in the healthy patients, but there was no significant difference in the Shannon diversity index between the groups. No significant difference was found in beta diversity between the groups. The core oral microbiota consisted of 67 bacterial species that were identified in all 24 healthy dogs. Our study provides further insight into the composition of the oral microbiota of healthy dogs and in dogs with oral tumors. |
| first_indexed | 2024-03-09T01:56:32Z |
| format | Article |
| id | doaj.art-ad4d6cd550c84fc8a210c92a38017964 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T01:56:32Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ad4d6cd550c84fc8a210c92a380179642023-12-08T15:10:24ZengMDPI AGAnimals2076-26152023-11-011323359410.3390/ani13233594A Comparison of the Oral Microbiota in Healthy Dogs and Dogs with Oral TumorsAnja Lisjak0Bruna Correa Lopes1Rachel Pilla2Ana Nemec3Jan S. Suchodolski4Nataša Tozon5Small Animal Clinic, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USASmall Animal Clinic, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USASmall Animal Clinic, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaThe aim of this study was to further describe the oral microbiota of healthy dogs by DNA shotgun sequencing and compare those to dogs with oral tumors. Oral swabs (representative of all niches of the oral cavity) were collected from healthy dogs (n = 24) and from dogs with different oral tumors (n = 7). DNA was extracted from the swabs and shotgun metagenomic sequencing was performed. Only minor differences in microbiota composition were observed between the two groups. At the phylum level, the Bacteroidota, Proteobacteria, Actinobacteriota, Desulfobacterota and Firmicutes were most abundant in both groups. Observed Operational Taxonomic Units—OTUs (species richness) was significantly higher in the healthy patients, but there was no significant difference in the Shannon diversity index between the groups. No significant difference was found in beta diversity between the groups. The core oral microbiota consisted of 67 bacterial species that were identified in all 24 healthy dogs. Our study provides further insight into the composition of the oral microbiota of healthy dogs and in dogs with oral tumors.https://www.mdpi.com/2076-2615/13/23/3594oral microbiotaDNA shotgun sequencingoral tumorsoral swabbiopsydogs |
| spellingShingle | Anja Lisjak Bruna Correa Lopes Rachel Pilla Ana Nemec Jan S. Suchodolski Nataša Tozon A Comparison of the Oral Microbiota in Healthy Dogs and Dogs with Oral Tumors Animals oral microbiota DNA shotgun sequencing oral tumors oral swab biopsy dogs |
| title | A Comparison of the Oral Microbiota in Healthy Dogs and Dogs with Oral Tumors |
| title_full | A Comparison of the Oral Microbiota in Healthy Dogs and Dogs with Oral Tumors |
| title_fullStr | A Comparison of the Oral Microbiota in Healthy Dogs and Dogs with Oral Tumors |
| title_full_unstemmed | A Comparison of the Oral Microbiota in Healthy Dogs and Dogs with Oral Tumors |
| title_short | A Comparison of the Oral Microbiota in Healthy Dogs and Dogs with Oral Tumors |
| title_sort | comparison of the oral microbiota in healthy dogs and dogs with oral tumors |
| topic | oral microbiota DNA shotgun sequencing oral tumors oral swab biopsy dogs |
| url | https://www.mdpi.com/2076-2615/13/23/3594 |
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