The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in Solution
Within the past decade, electrospray ionization mass spectrometry (ESI-MS) has rapidly occupied a prominent position for liquid-phase mechanistic studies due to its intrinsic advantages allowing for efficient “fishing” (rapid, sensitive, specific and simultaneous detection/identification) of multipl...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-09-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/17/10/11507 |
| _version_ | 1828395730566381568 |
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| author | Bin Guo Bo Chen Ling Tang Hua Wang Le Zeng Peng Zhou Yu Yuan Weitao Zhu |
| author_facet | Bin Guo Bo Chen Ling Tang Hua Wang Le Zeng Peng Zhou Yu Yuan Weitao Zhu |
| author_sort | Bin Guo |
| collection | DOAJ |
| description | Within the past decade, electrospray ionization mass spectrometry (ESI-MS) has rapidly occupied a prominent position for liquid-phase mechanistic studies due to its intrinsic advantages allowing for efficient “fishing” (rapid, sensitive, specific and simultaneous detection/identification) of multiple intermediates and products directly from a “real-world” solution. In this review we attempt to offer a comprehensive overview of the ESI-MS-based methodologies and strategies developed up to date to study reactive species in reaction solutions. A full description of general issues involved with probing reacting species from complex (bio)chemical reaction systems is briefly covered, including the potential sources of reactive intermediate (metabolite) generation, analytical aspects and challenges, basic rudiments of ESI-MS and the state-of-the-art technology. The main purpose of the present review is to highlight the utility of ESI-MS and its expanding role in probing reactive intermediates from various reactions in solution, with special focus on current progress in ESI-MS-based approaches for improving throughput, testing reality and real-time detection by using newly developed MS instruments and emerging ionization sources (such as ambient ESI techniques). In addition, the limitations of modern ESI-MS in detecting intermediates in organic reactions is also discussed. |
| first_indexed | 2024-12-10T08:18:03Z |
| format | Article |
| id | doaj.art-ad4d8705f9634a51b0fba77dab6f1c80 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T08:18:03Z |
| publishDate | 2012-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ad4d8705f9634a51b0fba77dab6f1c802022-12-22T01:56:25ZengMDPI AGMolecules1420-30492012-09-011710115071153710.3390/molecules171011507The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in SolutionBin GuoBo ChenLing TangHua WangLe ZengPeng ZhouYu YuanWeitao ZhuWithin the past decade, electrospray ionization mass spectrometry (ESI-MS) has rapidly occupied a prominent position for liquid-phase mechanistic studies due to its intrinsic advantages allowing for efficient “fishing” (rapid, sensitive, specific and simultaneous detection/identification) of multiple intermediates and products directly from a “real-world” solution. In this review we attempt to offer a comprehensive overview of the ESI-MS-based methodologies and strategies developed up to date to study reactive species in reaction solutions. A full description of general issues involved with probing reacting species from complex (bio)chemical reaction systems is briefly covered, including the potential sources of reactive intermediate (metabolite) generation, analytical aspects and challenges, basic rudiments of ESI-MS and the state-of-the-art technology. The main purpose of the present review is to highlight the utility of ESI-MS and its expanding role in probing reactive intermediates from various reactions in solution, with special focus on current progress in ESI-MS-based approaches for improving throughput, testing reality and real-time detection by using newly developed MS instruments and emerging ionization sources (such as ambient ESI techniques). In addition, the limitations of modern ESI-MS in detecting intermediates in organic reactions is also discussed.http://www.mdpi.com/1420-3049/17/10/11507intermediatesreactive metaboliteelectrosprayambient ionizationmass spectrometrychemical trappingsolution |
| spellingShingle | Bin Guo Bo Chen Ling Tang Hua Wang Le Zeng Peng Zhou Yu Yuan Weitao Zhu The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in Solution Molecules intermediates reactive metabolite electrospray ambient ionization mass spectrometry chemical trapping solution |
| title | The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in Solution |
| title_full | The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in Solution |
| title_fullStr | The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in Solution |
| title_full_unstemmed | The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in Solution |
| title_short | The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in Solution |
| title_sort | expanding role of electrospray ionization mass spectrometry for probing reactive intermediates in solution |
| topic | intermediates reactive metabolite electrospray ambient ionization mass spectrometry chemical trapping solution |
| url | http://www.mdpi.com/1420-3049/17/10/11507 |
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