Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina.
Regulation of ion and pH homeostasis is essential for normal neuronal function. The sodium-driven chloride bicarbonate exchanger NCBE (Slc4a10), a member of the SLC4 family of bicarbonate transporters, uses the transmembrane gradient of sodium to drive cellular net uptake of bicarbonate and to extru...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23056253/?tool=EBI |
| _version_ | 1818435288268013568 |
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| author | Gerrit Hilgen Antje K Huebner Naoyuki Tanimoto Vithiyanjali Sothilingam Christina Seide Marina Garcia Garrido Karl-Friedrich Schmidt Mathias W Seeliger Siegrid Löwel Reto Weiler Christian A Hübner Karin Dedek |
| author_facet | Gerrit Hilgen Antje K Huebner Naoyuki Tanimoto Vithiyanjali Sothilingam Christina Seide Marina Garcia Garrido Karl-Friedrich Schmidt Mathias W Seeliger Siegrid Löwel Reto Weiler Christian A Hübner Karin Dedek |
| author_sort | Gerrit Hilgen |
| collection | DOAJ |
| description | Regulation of ion and pH homeostasis is essential for normal neuronal function. The sodium-driven chloride bicarbonate exchanger NCBE (Slc4a10), a member of the SLC4 family of bicarbonate transporters, uses the transmembrane gradient of sodium to drive cellular net uptake of bicarbonate and to extrude chloride, thereby modulating both intracellular pH (pH(i)) and chloride concentration ([Cl(-)](i)) in neurons. Here we show that NCBE is strongly expressed in the retina. As GABA(A) receptors conduct both chloride and bicarbonate, we hypothesized that NCBE may be relevant for GABAergic transmission in the retina. Importantly, we found a differential expression of NCBE in bipolar cells: whereas NCBE was expressed on ON and OFF bipolar cell axon terminals, it only localized to dendrites of OFF bipolar cells. On these compartments, NCBE colocalized with the main neuronal chloride extruder KCC2, which renders GABA hyperpolarizing. NCBE was also expressed in starburst amacrine cells, but was absent from neurons known to depolarize in response to GABA, like horizontal cells. Mice lacking NCBE showed decreased visual acuity and contrast sensitivity in behavioral experiments and smaller b-wave amplitudes and longer latencies in electroretinograms. Ganglion cells from NCBE-deficient mice also showed altered temporal response properties. In summary, our data suggest that NCBE may serve to maintain intracellular chloride and bicarbonate concentration in retinal neurons. Consequently, lack of NCBE in the retina may result in changes in pH(i) regulation and chloride-dependent inhibition, leading to altered signal transmission and impaired visual function. |
| first_indexed | 2024-12-14T16:50:30Z |
| format | Article |
| id | doaj.art-ad4f535473474f3c861e01bdc96b4f29 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T16:50:30Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ad4f535473474f3c861e01bdc96b4f292022-12-21T22:54:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01710e4615510.1371/journal.pone.0046155Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina.Gerrit HilgenAntje K HuebnerNaoyuki TanimotoVithiyanjali SothilingamChristina SeideMarina Garcia GarridoKarl-Friedrich SchmidtMathias W SeeligerSiegrid LöwelReto WeilerChristian A HübnerKarin DedekRegulation of ion and pH homeostasis is essential for normal neuronal function. The sodium-driven chloride bicarbonate exchanger NCBE (Slc4a10), a member of the SLC4 family of bicarbonate transporters, uses the transmembrane gradient of sodium to drive cellular net uptake of bicarbonate and to extrude chloride, thereby modulating both intracellular pH (pH(i)) and chloride concentration ([Cl(-)](i)) in neurons. Here we show that NCBE is strongly expressed in the retina. As GABA(A) receptors conduct both chloride and bicarbonate, we hypothesized that NCBE may be relevant for GABAergic transmission in the retina. Importantly, we found a differential expression of NCBE in bipolar cells: whereas NCBE was expressed on ON and OFF bipolar cell axon terminals, it only localized to dendrites of OFF bipolar cells. On these compartments, NCBE colocalized with the main neuronal chloride extruder KCC2, which renders GABA hyperpolarizing. NCBE was also expressed in starburst amacrine cells, but was absent from neurons known to depolarize in response to GABA, like horizontal cells. Mice lacking NCBE showed decreased visual acuity and contrast sensitivity in behavioral experiments and smaller b-wave amplitudes and longer latencies in electroretinograms. Ganglion cells from NCBE-deficient mice also showed altered temporal response properties. In summary, our data suggest that NCBE may serve to maintain intracellular chloride and bicarbonate concentration in retinal neurons. Consequently, lack of NCBE in the retina may result in changes in pH(i) regulation and chloride-dependent inhibition, leading to altered signal transmission and impaired visual function.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23056253/?tool=EBI |
| spellingShingle | Gerrit Hilgen Antje K Huebner Naoyuki Tanimoto Vithiyanjali Sothilingam Christina Seide Marina Garcia Garrido Karl-Friedrich Schmidt Mathias W Seeliger Siegrid Löwel Reto Weiler Christian A Hübner Karin Dedek Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina. PLoS ONE |
| title | Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina. |
| title_full | Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina. |
| title_fullStr | Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina. |
| title_full_unstemmed | Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina. |
| title_short | Lack of the sodium-driven chloride bicarbonate exchanger NCBE impairs visual function in the mouse retina. |
| title_sort | lack of the sodium driven chloride bicarbonate exchanger ncbe impairs visual function in the mouse retina |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23056253/?tool=EBI |
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