Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells
The suppressive effects of flavonoids on macrophage-associated adipocyte inflammation in a differentiated murine preadipocyte cell line (3T3-L1) co-cultured with a murine macrophage cell line (RAW264.7) were evaluated. Extracellular lipid accumulation was investigated via Oil Red O staining. The exp...
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MDPI AG
2022-12-01
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| Series: | Plants |
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| author | Dahae Lee Sukyong Hong Kiwon Jung Sungyoul Choi Ki Sung Kang |
| author_facet | Dahae Lee Sukyong Hong Kiwon Jung Sungyoul Choi Ki Sung Kang |
| author_sort | Dahae Lee |
| collection | DOAJ |
| description | The suppressive effects of flavonoids on macrophage-associated adipocyte inflammation in a differentiated murine preadipocyte cell line (3T3-L1) co-cultured with a murine macrophage cell line (RAW264.7) were evaluated. Extracellular lipid accumulation was investigated via Oil Red O staining. The expression levels of adipogenesis- and inflammation-associated proteins, including CCAAT/enhancer-binding protein (C/EBP)-α, inducible nitric oxide synthase (iNOS), C/EBPβ, peroxisome proliferator-activated receptor γ (PPARγ), and cyclooxygenase-2 (COX-2), were determined via Western blotting. Proinflammatory cytokines, including monocyte chemoattractant protein 1 (MCP-1) and interleukin-6 (IL-6), were assessed using enzyme-linked immunosorbent assay kits. We found that silybin, formononetin, and diosmetin inhibited lipid accumulation and production of proinflammatory cytokines in the co-cultures of 3T3-L1 and RAW264.7 cells. Moreover, they inhibited the protein expression of PPARγ, C/EBPα, COX-2, C/EBPβ, and iNOS in the co-cultures of 3T3-L1 and RAW264.7 cells. These data support that silybin, formononetin, and diosmetin inhibit macrophage-associated adipocyte inflammation and lipid accumulation. |
| first_indexed | 2024-03-09T15:56:43Z |
| format | Article |
| id | doaj.art-ad50a72f121248c5a5ce04584704d9dd |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T15:56:43Z |
| publishDate | 2022-12-01 |
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| series | Plants |
| spelling | doaj.art-ad50a72f121248c5a5ce04584704d9dd2023-11-24T17:29:16ZengMDPI AGPlants2223-77472022-12-011124355210.3390/plants11243552Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 CellsDahae Lee0Sukyong Hong1Kiwon Jung2Sungyoul Choi3Ki Sung Kang4Department of Preventive Medicine, College of Korean Medicine, Gachon University, Seongnam 13120, Republic of KoreaCollege of Pharmacy, CHA University, Sungnam 13844, Republic of KoreaCollege of Pharmacy, CHA University, Sungnam 13844, Republic of KoreaDepartment of Neuropsychiatry, College of Korean Medicine, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Preventive Medicine, College of Korean Medicine, Gachon University, Seongnam 13120, Republic of KoreaThe suppressive effects of flavonoids on macrophage-associated adipocyte inflammation in a differentiated murine preadipocyte cell line (3T3-L1) co-cultured with a murine macrophage cell line (RAW264.7) were evaluated. Extracellular lipid accumulation was investigated via Oil Red O staining. The expression levels of adipogenesis- and inflammation-associated proteins, including CCAAT/enhancer-binding protein (C/EBP)-α, inducible nitric oxide synthase (iNOS), C/EBPβ, peroxisome proliferator-activated receptor γ (PPARγ), and cyclooxygenase-2 (COX-2), were determined via Western blotting. Proinflammatory cytokines, including monocyte chemoattractant protein 1 (MCP-1) and interleukin-6 (IL-6), were assessed using enzyme-linked immunosorbent assay kits. We found that silybin, formononetin, and diosmetin inhibited lipid accumulation and production of proinflammatory cytokines in the co-cultures of 3T3-L1 and RAW264.7 cells. Moreover, they inhibited the protein expression of PPARγ, C/EBPα, COX-2, C/EBPβ, and iNOS in the co-cultures of 3T3-L1 and RAW264.7 cells. These data support that silybin, formononetin, and diosmetin inhibit macrophage-associated adipocyte inflammation and lipid accumulation.https://www.mdpi.com/2223-7747/11/24/3552flavonoidadipocytesmacrophagesadipogenesisinflammation |
| spellingShingle | Dahae Lee Sukyong Hong Kiwon Jung Sungyoul Choi Ki Sung Kang Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells Plants flavonoid adipocytes macrophages adipogenesis inflammation |
| title | Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells |
| title_full | Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells |
| title_fullStr | Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells |
| title_full_unstemmed | Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells |
| title_short | Suppressive Effects of Flavonoids on Macrophage-Associated Adipocyte Inflammation in a Differentiated Murine Preadipocyte 3T3-L1 Cells Co-Cultured with a Murine Macrophage RAW264.7 Cells |
| title_sort | suppressive effects of flavonoids on macrophage associated adipocyte inflammation in a differentiated murine preadipocyte 3t3 l1 cells co cultured with a murine macrophage raw264 7 cells |
| topic | flavonoid adipocytes macrophages adipogenesis inflammation |
| url | https://www.mdpi.com/2223-7747/11/24/3552 |
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