Determination of Benzo(α)pyrene in Infant Formula Using High Performance Liquid Chromatography and Dispersive Liquid–Liquid Microextraction
Background: Benzo(α)pyrene (BaP) is one of the most familiar polycyclic aromatic hydrocarbons (PAHs) to evaluate food safety and quality and can be present in infant formulas An analytical method for the extraction and quantification of BaP in infant formula milk has been established by dispersive l...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2023-04-01
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| Series: | Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://ps.tbzmed.ac.ir/PDF/ps-29-246.pdf |
| _version_ | 1797836036783472640 |
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| author | Akram Faridi Mahboob Nemati |
| author_facet | Akram Faridi Mahboob Nemati |
| author_sort | Akram Faridi |
| collection | DOAJ |
| description | Background: Benzo(α)pyrene (BaP) is one of the most familiar polycyclic aromatic hydrocarbons (PAHs) to evaluate food safety and quality and can be present in infant formulas An analytical method for the extraction and quantification of BaP in infant formula milk has been established by dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography with fluorescence detector. Methods: BaP was first extracted from matrices of infant formula milk via acetonitrile and then DLLME was used for further purification and preconcentration of target analyte. Results: Under the optimum extraction conditions, 150 µL of dichloromethane as extraction solvent, 3 mL acetonitrile as disperser and cleaner solvent and 1 mL volume sample, the accuracy of the method was between 89-97%. The limits of detection and quantification were 0.12 and 0.35 ng/ml, respectively. There was a linear relation (R2=0.998) between chromatographic peak area and concentrations in the range of 0.5 to 15 ng/mL. Conclusion: The proposed method is applicable to the quantification of BaP in infant formula milk with acceptable accuracy and precision. |
| first_indexed | 2024-04-09T15:02:09Z |
| format | Article |
| id | doaj.art-ad558efb7a4947c9b4d36c60cbe10790 |
| institution | Directory Open Access Journal |
| issn | 2383-2886 |
| language | English |
| last_indexed | 2024-04-09T15:02:09Z |
| publishDate | 2023-04-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical Sciences |
| spelling | doaj.art-ad558efb7a4947c9b4d36c60cbe107902023-05-01T10:23:35ZengTabriz University of Medical SciencesPharmaceutical Sciences2383-28862023-04-0129224625110.34172/PS.2022.14ps-34907Determination of Benzo(α)pyrene in Infant Formula Using High Performance Liquid Chromatography and Dispersive Liquid–Liquid MicroextractionAkram Faridi0Mahboob Nemati1Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Background: Benzo(α)pyrene (BaP) is one of the most familiar polycyclic aromatic hydrocarbons (PAHs) to evaluate food safety and quality and can be present in infant formulas An analytical method for the extraction and quantification of BaP in infant formula milk has been established by dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography with fluorescence detector. Methods: BaP was first extracted from matrices of infant formula milk via acetonitrile and then DLLME was used for further purification and preconcentration of target analyte. Results: Under the optimum extraction conditions, 150 µL of dichloromethane as extraction solvent, 3 mL acetonitrile as disperser and cleaner solvent and 1 mL volume sample, the accuracy of the method was between 89-97%. The limits of detection and quantification were 0.12 and 0.35 ng/ml, respectively. There was a linear relation (R2=0.998) between chromatographic peak area and concentrations in the range of 0.5 to 15 ng/mL. Conclusion: The proposed method is applicable to the quantification of BaP in infant formula milk with acceptable accuracy and precision.https://ps.tbzmed.ac.ir/PDF/ps-29-246.pdfbenzo (α) pyrenehplcinfant formula milkmicroextraction |
| spellingShingle | Akram Faridi Mahboob Nemati Determination of Benzo(α)pyrene in Infant Formula Using High Performance Liquid Chromatography and Dispersive Liquid–Liquid Microextraction Pharmaceutical Sciences benzo (α) pyrene hplc infant formula milk microextraction |
| title | Determination of Benzo(α)pyrene in Infant Formula Using High Performance Liquid Chromatography and Dispersive Liquid–Liquid Microextraction |
| title_full | Determination of Benzo(α)pyrene in Infant Formula Using High Performance Liquid Chromatography and Dispersive Liquid–Liquid Microextraction |
| title_fullStr | Determination of Benzo(α)pyrene in Infant Formula Using High Performance Liquid Chromatography and Dispersive Liquid–Liquid Microextraction |
| title_full_unstemmed | Determination of Benzo(α)pyrene in Infant Formula Using High Performance Liquid Chromatography and Dispersive Liquid–Liquid Microextraction |
| title_short | Determination of Benzo(α)pyrene in Infant Formula Using High Performance Liquid Chromatography and Dispersive Liquid–Liquid Microextraction |
| title_sort | determination of benzo α pyrene in infant formula using high performance liquid chromatography and dispersive liquid liquid microextraction |
| topic | benzo (α) pyrene hplc infant formula milk microextraction |
| url | https://ps.tbzmed.ac.ir/PDF/ps-29-246.pdf |
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