Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity
Blaps rynchopetera Fairmaire has a long history of use as a folk medicine in China for treating fever, cough, gastritis, boils, and tumors. In the present study, the content analyses, monosaccharide composition analyses, and the macrophage phagocytic activity of rynchopetera polysaccharides (RPS) we...
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| Format: | Article |
| Language: | English |
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De Gruyter
2021-03-01
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| Series: | Open Chemistry |
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| Online Access: | https://doi.org/10.1515/chem-2021-0035 |
| _version_ | 1818509974359244800 |
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| author | Wang Ying Yang Yin-He Luo Qing Hu Yuan Lu Qian Yu Wan-Xin Chen Jin-Hu Wang Yu-Jia Wang Mu-Ling Zhao Yu Xiao Huai |
| author_facet | Wang Ying Yang Yin-He Luo Qing Hu Yuan Lu Qian Yu Wan-Xin Chen Jin-Hu Wang Yu-Jia Wang Mu-Ling Zhao Yu Xiao Huai |
| author_sort | Wang Ying |
| collection | DOAJ |
| description | Blaps rynchopetera Fairmaire has a long history of use as a folk medicine in China for treating fever, cough, gastritis, boils, and tumors. In the present study, the content analyses, monosaccharide composition analyses, and the macrophage phagocytic activity of rynchopetera polysaccharides (RPS) were reported. B. rhynchoptera is rich in polysaccharides (content value 3.97%). Through PMP (1-phenyl-3-methyl-5-pyrazolone) pre-column derivatization and high performance liquid chromatography (HPLC) testing, the results showed that RPS consist of 8 known monosaccharides, including D-mannose (Man), Rhamnose (Rha), D-glucuronic acid (GlcUA), D-galacturonic acid (GalUA), D-glucose (Glc), D-galactose (Gal), Arabinose (Ara), and Fucose (Fuc), with the total content of 171.70 mg g−1 and Glc has the highest content of 45.40 mg g−1. The phagocytic ability of mouse peritoneal macrophages was investigated after RPS stimulating alone and combined with lipopolysaccharide (LPS). RPS played an important role in the engulfment of mouse peritoneal macrophages and can significantly enhance the phagocytic ability of macrophages. However, no synergistic effects were observed when RPS combined with LPS. |
| first_indexed | 2024-12-10T22:52:49Z |
| format | Article |
| id | doaj.art-ad58237692494a40b9c1ef37c9a1f1ae |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-12-10T22:52:49Z |
| publishDate | 2021-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-ad58237692494a40b9c1ef37c9a1f1ae2022-12-22T01:30:22ZengDe GruyterOpen Chemistry2391-54202021-03-0119133834610.1515/chem-2021-0035Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activityWang Ying0Yang Yin-He1Luo Qing2Hu Yuan3Lu Qian4Yu Wan-Xin5Chen Jin-Hu6Wang Yu-Jia7Wang Mu-Ling8Zhao Yu9Xiao Huai10Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, 671000, People’s Republic of ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, 671000, People’s Republic of ChinaDepartment of Clinical Trial, IQVIA RDS Co., Ltd., Shanghai 200032, People’s Republic of ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, 671000, People’s Republic of ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, 671000, People’s Republic of ChinaDepartment of Gene Therapy, Staidson Biopharmaceutical Co., Ltd., Beijing 100176, People’s Republic of ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, 671000, People’s Republic of ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, 671000, People’s Republic of ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, 671000, People’s Republic of ChinaNational-Local Joint Engineering Research Center of Entomoceutics, Dali University, Dali, 6710030, People’s Republic of ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, 671000, People’s Republic of ChinaBlaps rynchopetera Fairmaire has a long history of use as a folk medicine in China for treating fever, cough, gastritis, boils, and tumors. In the present study, the content analyses, monosaccharide composition analyses, and the macrophage phagocytic activity of rynchopetera polysaccharides (RPS) were reported. B. rhynchoptera is rich in polysaccharides (content value 3.97%). Through PMP (1-phenyl-3-methyl-5-pyrazolone) pre-column derivatization and high performance liquid chromatography (HPLC) testing, the results showed that RPS consist of 8 known monosaccharides, including D-mannose (Man), Rhamnose (Rha), D-glucuronic acid (GlcUA), D-galacturonic acid (GalUA), D-glucose (Glc), D-galactose (Gal), Arabinose (Ara), and Fucose (Fuc), with the total content of 171.70 mg g−1 and Glc has the highest content of 45.40 mg g−1. The phagocytic ability of mouse peritoneal macrophages was investigated after RPS stimulating alone and combined with lipopolysaccharide (LPS). RPS played an important role in the engulfment of mouse peritoneal macrophages and can significantly enhance the phagocytic ability of macrophages. However, no synergistic effects were observed when RPS combined with LPS.https://doi.org/10.1515/chem-2021-0035blaps rynchopeterapolysaccharidescontent determinationmonosaccharide compositionmacrophage phagocytic activity |
| spellingShingle | Wang Ying Yang Yin-He Luo Qing Hu Yuan Lu Qian Yu Wan-Xin Chen Jin-Hu Wang Yu-Jia Wang Mu-Ling Zhao Yu Xiao Huai Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity Open Chemistry blaps rynchopetera polysaccharides content determination monosaccharide composition macrophage phagocytic activity |
| title | Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity |
| title_full | Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity |
| title_fullStr | Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity |
| title_full_unstemmed | Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity |
| title_short | Content and composition analysis of polysaccharides from Blaps rynchopetera and its macrophage phagocytic activity |
| title_sort | content and composition analysis of polysaccharides from blaps rynchopetera and its macrophage phagocytic activity |
| topic | blaps rynchopetera polysaccharides content determination monosaccharide composition macrophage phagocytic activity |
| url | https://doi.org/10.1515/chem-2021-0035 |
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