MKK4 variants rs3826392 and rs3809728 are associated with susceptibility and clinicopathological features in colorectal cancer patients
Objective(s): The mitogen-activated protein kinase kinase 4 (MKK4) plays a key role in several processes like inflammation, apoptosis, and tumorigenesis. Several authors have proposed that genetic variations in these genes may alter their expression with subsequent cancer risk. This study aimed to e...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-08-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_18466_c0e4a052275af6639aa289130d350391.pdf |
| _version_ | 1818883222175481856 |
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| author | Kimberly Martínez-Casillas Anilú Margarita Saucedo-Sariñana Patricio Barros-Núñez Martha Patricia Gallegos Arreola Tomas Daniel Pineda Razo María Eugenia Marín-Contreras Silvia Esperanza Flores-Martínez Mónica Alejandra Rosales-Reynoso |
| author_facet | Kimberly Martínez-Casillas Anilú Margarita Saucedo-Sariñana Patricio Barros-Núñez Martha Patricia Gallegos Arreola Tomas Daniel Pineda Razo María Eugenia Marín-Contreras Silvia Esperanza Flores-Martínez Mónica Alejandra Rosales-Reynoso |
| author_sort | Kimberly Martínez-Casillas |
| collection | DOAJ |
| description | Objective(s): The mitogen-activated protein kinase kinase 4 (MKK4) plays a key role in several processes like inflammation, apoptosis, and tumorigenesis. Several authors have proposed that genetic variations in these genes may alter their expression with subsequent cancer risk. This study aimed to examine the possible association of MKK4 rs3826392 and rs3809728 variants in Mexican patients with colorectal cancer (CRC). These variants were also compared with clinical features as sex, age, TNM stage, and tumor location.Materials and Methods: The study included genomic DNA from 218 control subjects and 250 patients. Genotyping of the MKK4 variants was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) procedure. Results: Individuals with A/T and T/T genotypes for the rs3809728 (-1044 A>T) variant showed a significantly increased risk for CRC (P=0.012 and 0.007, respectively); while individuals with the G/G genotype for the rs3826392 (-1304 T>G) variant showed a decreased risk for CRC (P=0.012). Genotypes of the MKK4 rs3809728 variant were also significantly related to colon localization and advanced TNM stage in CRC patients. T-T haplotype (rs3826392 and rs3809728) of the MKK4 gene was associated with risk in patients with CRC.Conclusion: The rs3826392 variant in the MKK4 gene could be a cancer protective factor, while the rs3809728 variant could be a risk factor. These variants play a significant role in CRC risk. |
| first_indexed | 2024-12-19T15:30:13Z |
| format | Article |
| id | doaj.art-ad582631b4b1430b8e588fb7a25c02ef |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-19T15:30:13Z |
| publishDate | 2021-08-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-ad582631b4b1430b8e588fb7a25c02ef2022-12-21T20:15:45ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742021-08-012481033104010.22038/ijbms.2021.56874.1269018466MKK4 variants rs3826392 and rs3809728 are associated with susceptibility and clinicopathological features in colorectal cancer patientsKimberly Martínez-Casillas0Anilú Margarita Saucedo-Sariñana1Patricio Barros-Núñez2Martha Patricia Gallegos Arreola3Tomas Daniel Pineda Razo4María Eugenia Marín-Contreras5Silvia Esperanza Flores-Martínez6Mónica Alejandra Rosales-Reynoso7División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, MéxicoDivisión de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, MéxicoUnidad de Investigación Seguimiento Enfermedades Metabólicas, Unidad Médica de Alta Especialidad Pediatría, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco. MéxicoDivisión de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, MéxicoServicio de Oncología Médica, Hospital de Especialidades, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, MéxicoServicio de Gastroenterología, Hospital de Especialidades, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, MéxicoDivisión de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, MéxicoDivisión de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, MéxicoObjective(s): The mitogen-activated protein kinase kinase 4 (MKK4) plays a key role in several processes like inflammation, apoptosis, and tumorigenesis. Several authors have proposed that genetic variations in these genes may alter their expression with subsequent cancer risk. This study aimed to examine the possible association of MKK4 rs3826392 and rs3809728 variants in Mexican patients with colorectal cancer (CRC). These variants were also compared with clinical features as sex, age, TNM stage, and tumor location.Materials and Methods: The study included genomic DNA from 218 control subjects and 250 patients. Genotyping of the MKK4 variants was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) procedure. Results: Individuals with A/T and T/T genotypes for the rs3809728 (-1044 A>T) variant showed a significantly increased risk for CRC (P=0.012 and 0.007, respectively); while individuals with the G/G genotype for the rs3826392 (-1304 T>G) variant showed a decreased risk for CRC (P=0.012). Genotypes of the MKK4 rs3809728 variant were also significantly related to colon localization and advanced TNM stage in CRC patients. T-T haplotype (rs3826392 and rs3809728) of the MKK4 gene was associated with risk in patients with CRC.Conclusion: The rs3826392 variant in the MKK4 gene could be a cancer protective factor, while the rs3809728 variant could be a risk factor. These variants play a significant role in CRC risk.https://ijbms.mums.ac.ir/article_18466_c0e4a052275af6639aa289130d350391.pdfcolorectal cancerhaplotypesmkk4susceptibilityvariants |
| spellingShingle | Kimberly Martínez-Casillas Anilú Margarita Saucedo-Sariñana Patricio Barros-Núñez Martha Patricia Gallegos Arreola Tomas Daniel Pineda Razo María Eugenia Marín-Contreras Silvia Esperanza Flores-Martínez Mónica Alejandra Rosales-Reynoso MKK4 variants rs3826392 and rs3809728 are associated with susceptibility and clinicopathological features in colorectal cancer patients Iranian Journal of Basic Medical Sciences colorectal cancer haplotypes mkk4 susceptibility variants |
| title | MKK4 variants rs3826392 and rs3809728 are associated with susceptibility and clinicopathological features in colorectal cancer patients |
| title_full | MKK4 variants rs3826392 and rs3809728 are associated with susceptibility and clinicopathological features in colorectal cancer patients |
| title_fullStr | MKK4 variants rs3826392 and rs3809728 are associated with susceptibility and clinicopathological features in colorectal cancer patients |
| title_full_unstemmed | MKK4 variants rs3826392 and rs3809728 are associated with susceptibility and clinicopathological features in colorectal cancer patients |
| title_short | MKK4 variants rs3826392 and rs3809728 are associated with susceptibility and clinicopathological features in colorectal cancer patients |
| title_sort | mkk4 variants rs3826392 and rs3809728 are associated with susceptibility and clinicopathological features in colorectal cancer patients |
| topic | colorectal cancer haplotypes mkk4 susceptibility variants |
| url | https://ijbms.mums.ac.ir/article_18466_c0e4a052275af6639aa289130d350391.pdf |
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