Diagnostic accuracy and turnaround time of the Xpert MTB/RIF assay in routine clinical practice.
The Xpert MTB/RIF assay was introduced for timely and accurate detection of tuberculosis (TB). The aim of this study was to determine the diagnostic accuracy and turnaround time (TAT) of Xpert MTB/RIF assay in clinical practice in South Korea. We retrospectively reviewed the medical records of patie...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3812224?pdf=render |
| _version_ | 1818898161390845952 |
|---|---|
| author | Nakwon Kwak Sun Mi Choi Jinwoo Lee Young Sik Park Chang-Hoon Lee Sang-Min Lee Chul-Gyu Yoo Young Whan Kim Sung Koo Han Jae-Joon Yim |
| author_facet | Nakwon Kwak Sun Mi Choi Jinwoo Lee Young Sik Park Chang-Hoon Lee Sang-Min Lee Chul-Gyu Yoo Young Whan Kim Sung Koo Han Jae-Joon Yim |
| author_sort | Nakwon Kwak |
| collection | DOAJ |
| description | The Xpert MTB/RIF assay was introduced for timely and accurate detection of tuberculosis (TB). The aim of this study was to determine the diagnostic accuracy and turnaround time (TAT) of Xpert MTB/RIF assay in clinical practice in South Korea. We retrospectively reviewed the medical records of patients in whom Xpert MTB/RIF assay using sputum were requested. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for the diagnosis of pulmonary tuberculosis (PTB) and detection of rifampicin resistance were calculated. In addition, TAT of Xpert MTB/RIF assay was compared with those of other tests. Total 681 patients in whom Xpert MTB/RIF assay was requested were included in the analysis. The sensitivity, specificity, PPV and NPV of Xpert MTB/RIF assay for diagnosis of PTB were 79.5% (124/156), 100.0% (505/505), 100.0% (124/124) and 94.0% (505/537), respectively. Those for the detection of rifampicin resistance were 57.1% (8/14), 100.0% (113/113), 100.0% (8/8) and 94.9% (113/119), respectively. The median TAT of Xpert MTB/RIF assay to the report of results and results confirmed by physicians in outpatient settings were 0 (0-1) and 6 (3-7) days, respectively. Median time to treatment after initial evaluation was 7 (4-9) days in patients with Xpert MTB/RIF assay, but was 21 (7-33.5) days in patients without Xpert MTB/RIF assay. Xpert MTB/RIF assay showed acceptable sensitivity and excellent specificity for the diagnosis of PTB and detection of rifampicin resistance in areas with intermediate TB burden. Additionally, the assay decreased time to the initiation of anti-TB drugs through shorter TAT. |
| first_indexed | 2024-12-19T19:27:40Z |
| format | Article |
| id | doaj.art-ad58c22df74f4df6a5de5132fc5499dc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T19:27:40Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ad58c22df74f4df6a5de5132fc5499dc2022-12-21T20:08:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7745610.1371/journal.pone.0077456Diagnostic accuracy and turnaround time of the Xpert MTB/RIF assay in routine clinical practice.Nakwon KwakSun Mi ChoiJinwoo LeeYoung Sik ParkChang-Hoon LeeSang-Min LeeChul-Gyu YooYoung Whan KimSung Koo HanJae-Joon YimThe Xpert MTB/RIF assay was introduced for timely and accurate detection of tuberculosis (TB). The aim of this study was to determine the diagnostic accuracy and turnaround time (TAT) of Xpert MTB/RIF assay in clinical practice in South Korea. We retrospectively reviewed the medical records of patients in whom Xpert MTB/RIF assay using sputum were requested. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for the diagnosis of pulmonary tuberculosis (PTB) and detection of rifampicin resistance were calculated. In addition, TAT of Xpert MTB/RIF assay was compared with those of other tests. Total 681 patients in whom Xpert MTB/RIF assay was requested were included in the analysis. The sensitivity, specificity, PPV and NPV of Xpert MTB/RIF assay for diagnosis of PTB were 79.5% (124/156), 100.0% (505/505), 100.0% (124/124) and 94.0% (505/537), respectively. Those for the detection of rifampicin resistance were 57.1% (8/14), 100.0% (113/113), 100.0% (8/8) and 94.9% (113/119), respectively. The median TAT of Xpert MTB/RIF assay to the report of results and results confirmed by physicians in outpatient settings were 0 (0-1) and 6 (3-7) days, respectively. Median time to treatment after initial evaluation was 7 (4-9) days in patients with Xpert MTB/RIF assay, but was 21 (7-33.5) days in patients without Xpert MTB/RIF assay. Xpert MTB/RIF assay showed acceptable sensitivity and excellent specificity for the diagnosis of PTB and detection of rifampicin resistance in areas with intermediate TB burden. Additionally, the assay decreased time to the initiation of anti-TB drugs through shorter TAT.http://europepmc.org/articles/PMC3812224?pdf=render |
| spellingShingle | Nakwon Kwak Sun Mi Choi Jinwoo Lee Young Sik Park Chang-Hoon Lee Sang-Min Lee Chul-Gyu Yoo Young Whan Kim Sung Koo Han Jae-Joon Yim Diagnostic accuracy and turnaround time of the Xpert MTB/RIF assay in routine clinical practice. PLoS ONE |
| title | Diagnostic accuracy and turnaround time of the Xpert MTB/RIF assay in routine clinical practice. |
| title_full | Diagnostic accuracy and turnaround time of the Xpert MTB/RIF assay in routine clinical practice. |
| title_fullStr | Diagnostic accuracy and turnaround time of the Xpert MTB/RIF assay in routine clinical practice. |
| title_full_unstemmed | Diagnostic accuracy and turnaround time of the Xpert MTB/RIF assay in routine clinical practice. |
| title_short | Diagnostic accuracy and turnaround time of the Xpert MTB/RIF assay in routine clinical practice. |
| title_sort | diagnostic accuracy and turnaround time of the xpert mtb rif assay in routine clinical practice |
| url | http://europepmc.org/articles/PMC3812224?pdf=render |
| work_keys_str_mv | AT nakwonkwak diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice AT sunmichoi diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice AT jinwoolee diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice AT youngsikpark diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice AT changhoonlee diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice AT sangminlee diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice AT chulgyuyoo diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice AT youngwhankim diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice AT sungkoohan diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice AT jaejoonyim diagnosticaccuracyandturnaroundtimeofthexpertmtbrifassayinroutineclinicalpractice |