Draft genome sequences of Bacillus pumilus 36R ATNSAL and B. safensis 13L LOBSAL, two potential candidate probiotic strains for shrimp aquaculture
ABSTRACT: Objectives: This work aimed to isolate bacterial strains with antagonist activity against Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (VPAHPND) that was isolated from outbreaks in Mexico. Here, we report the draft genome sequences of two antagon...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716522002314 |
| _version_ | 1828163116718882816 |
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| author | Ricardo Sánchez-Díaz Zinnia Judith Molina-Garza Lucía Elizabeth Cruz-Suárez Joseph Selvin George Seghal Kiran Bruno Gómez-Gil Lucio Galaviz-Silva José Cuauhtémoc Ibarra-Gámez |
| author_facet | Ricardo Sánchez-Díaz Zinnia Judith Molina-Garza Lucía Elizabeth Cruz-Suárez Joseph Selvin George Seghal Kiran Bruno Gómez-Gil Lucio Galaviz-Silva José Cuauhtémoc Ibarra-Gámez |
| author_sort | Ricardo Sánchez-Díaz |
| collection | DOAJ |
| description | ABSTRACT: Objectives: This work aimed to isolate bacterial strains with antagonist activity against Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (VPAHPND) that was isolated from outbreaks in Mexico. Here, we report the draft genome sequences of two antagonistic strains, isolated from saline sediment in Sonora, Mexico. Methods: Cross-streak and well diffusion tests were employed to find the bacterial strains with higher inhibitory activity against VPAHPND. The whole genomes of B. pumilus 36R ATNSAL and B. safensis 13L LOBSAL were sequenced using Ion TorrentTM (PGM) and Illumina MiseqTM platforms, respectively. Annotation was performed using the RAST server, and the genes involved in the biosynthesis of bacterial secondary metabolites were predicted using antiSMASH. Results: Two bacterial isolates, B. safensis 13L LOBSAL and B. pumilus 36R ATNSAL, were chosen based on their strong antagonistic profiles. The genome of 36R ATNSAL was 3.94 Mbp in length and contained 3824 genes and a total of 4116 coding sequences (CDSs); the genome of 13L LOBSAL was 3.68 Mbp and contained 3619 genes and 3688 CDSs. Twenty-eight and 32 biosynthetic gene clusters responsible for putative antimicrobial metabolite production were identified in 36R ATNSAL and 13L LOBSAL, respectively. Conclusions: The two strains 13L LOBSAL and 36R ATNSAL showed excellent probiotic profiles in vitro. The genome sequences will help with the mining and reconstruction of metabolic pathways in Bacillus strains. Genome sequence–guided strain improvement could augment the probiotic potential of Bacillus strains for applications in shrimp aquaculture. |
| first_indexed | 2024-04-12T01:05:50Z |
| format | Article |
| id | doaj.art-ad5a9acfaa2149c984193ff0dade1357 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-12T01:05:50Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-ad5a9acfaa2149c984193ff0dade13572022-12-22T03:54:17ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-12-0131304308Draft genome sequences of Bacillus pumilus 36R ATNSAL and B. safensis 13L LOBSAL, two potential candidate probiotic strains for shrimp aquacultureRicardo Sánchez-Díaz0Zinnia Judith Molina-Garza1Lucía Elizabeth Cruz-Suárez2Joseph Selvin3George Seghal Kiran4Bruno Gómez-Gil5Lucio Galaviz-Silva6José Cuauhtémoc Ibarra-Gámez7Technological Institute of Sonora (ITSON), 5 de Febrero 818 Sur, Col. Centro, Ciudad Obregón, Sonora,CP 85000, MéxicoAutonomous University of Nuevo Leon (UANL), Ave. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza, Nuevo León, CP 66455, MexicoAutonomous University of Nuevo Leon (UANL), Ave. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza, Nuevo León, CP 66455, MexicoDepartment of Microbiology, Pondicherry University, Puducherry, IndiaDepartment of Food Science and Technology, Pondicherry University, Puducherry, IndiaResearch Center for Food and Development (CIAD), Mazatlan Unit, Av. Sábalo Cerritos S/N, Mazatlán, Sinaloa, C.P. 82112. MexicoAutonomous University of Nuevo Leon (UANL), Ave. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza, Nuevo León, CP 66455, Mexico; Alternative corresponding author at: Autonomous University of Nuevo León (UANL), Ave. Universidad, S/N, Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, CP 66455, Mexico.Technological Institute of Sonora (ITSON), 5 de Febrero 818 Sur, Col. Centro, Ciudad Obregón, Sonora,CP 85000, México; Corresponding author at: Technological Institute of Sonora, 5 de Febrero 818 Sur, Col. Centro, Ciudad Obregón, Sonora, CP 85000, Mexico.ABSTRACT: Objectives: This work aimed to isolate bacterial strains with antagonist activity against Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (VPAHPND) that was isolated from outbreaks in Mexico. Here, we report the draft genome sequences of two antagonistic strains, isolated from saline sediment in Sonora, Mexico. Methods: Cross-streak and well diffusion tests were employed to find the bacterial strains with higher inhibitory activity against VPAHPND. The whole genomes of B. pumilus 36R ATNSAL and B. safensis 13L LOBSAL were sequenced using Ion TorrentTM (PGM) and Illumina MiseqTM platforms, respectively. Annotation was performed using the RAST server, and the genes involved in the biosynthesis of bacterial secondary metabolites were predicted using antiSMASH. Results: Two bacterial isolates, B. safensis 13L LOBSAL and B. pumilus 36R ATNSAL, were chosen based on their strong antagonistic profiles. The genome of 36R ATNSAL was 3.94 Mbp in length and contained 3824 genes and a total of 4116 coding sequences (CDSs); the genome of 13L LOBSAL was 3.68 Mbp and contained 3619 genes and 3688 CDSs. Twenty-eight and 32 biosynthetic gene clusters responsible for putative antimicrobial metabolite production were identified in 36R ATNSAL and 13L LOBSAL, respectively. Conclusions: The two strains 13L LOBSAL and 36R ATNSAL showed excellent probiotic profiles in vitro. The genome sequences will help with the mining and reconstruction of metabolic pathways in Bacillus strains. Genome sequence–guided strain improvement could augment the probiotic potential of Bacillus strains for applications in shrimp aquaculture.http://www.sciencedirect.com/science/article/pii/S2213716522002314Bacillus pumilusBacillus safensisAntibacterialProbioticShrimp aquacultureAHPND |
| spellingShingle | Ricardo Sánchez-Díaz Zinnia Judith Molina-Garza Lucía Elizabeth Cruz-Suárez Joseph Selvin George Seghal Kiran Bruno Gómez-Gil Lucio Galaviz-Silva José Cuauhtémoc Ibarra-Gámez Draft genome sequences of Bacillus pumilus 36R ATNSAL and B. safensis 13L LOBSAL, two potential candidate probiotic strains for shrimp aquaculture Journal of Global Antimicrobial Resistance Bacillus pumilus Bacillus safensis Antibacterial Probiotic Shrimp aquaculture AHPND |
| title | Draft genome sequences of Bacillus pumilus 36R ATNSAL and B. safensis 13L LOBSAL, two potential candidate probiotic strains for shrimp aquaculture |
| title_full | Draft genome sequences of Bacillus pumilus 36R ATNSAL and B. safensis 13L LOBSAL, two potential candidate probiotic strains for shrimp aquaculture |
| title_fullStr | Draft genome sequences of Bacillus pumilus 36R ATNSAL and B. safensis 13L LOBSAL, two potential candidate probiotic strains for shrimp aquaculture |
| title_full_unstemmed | Draft genome sequences of Bacillus pumilus 36R ATNSAL and B. safensis 13L LOBSAL, two potential candidate probiotic strains for shrimp aquaculture |
| title_short | Draft genome sequences of Bacillus pumilus 36R ATNSAL and B. safensis 13L LOBSAL, two potential candidate probiotic strains for shrimp aquaculture |
| title_sort | draft genome sequences of bacillus pumilus 36r atnsal and b safensis 13l lobsal two potential candidate probiotic strains for shrimp aquaculture |
| topic | Bacillus pumilus Bacillus safensis Antibacterial Probiotic Shrimp aquaculture AHPND |
| url | http://www.sciencedirect.com/science/article/pii/S2213716522002314 |
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