Genetic diversity assessment and selection of Bradyrhizobium strains for Inceptisols based on symbiotic performance
The symbiotic association of pigeonpea (Cajanus cajan (L.) Millsp.) with rhizobia plays a pivotal role in plants’ growth and development. The present study was carried out at Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh during 2018–2020 to explore the divers...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2023-10-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/140808 |
| _version_ | 1797663360272039936 |
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| author | SANTOSH KUMAR PREETI SINGH SHIV CHARAN KUMAR NITISH RANJAN PRAKASH UMAKANT BANJARE ARUN KUMAR PATEL VISHAL TYAGI RAMESH KUMAR SINGH |
| author_facet | SANTOSH KUMAR PREETI SINGH SHIV CHARAN KUMAR NITISH RANJAN PRAKASH UMAKANT BANJARE ARUN KUMAR PATEL VISHAL TYAGI RAMESH KUMAR SINGH |
| author_sort | SANTOSH KUMAR |
| collection | DOAJ |
| description |
The symbiotic association of pigeonpea (Cajanus cajan (L.) Millsp.) with rhizobia plays a pivotal role in plants’ growth and development. The present study was carried out at Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh during 2018–2020 to explore the diversity of rhizobia present in the Cajanus cajan root under Inceptisols and identify an elite strain that exhibits exceptional plant growth promotion and of nitrogenase activity for efficient nitrogen fixation. The bacterial identification using 16s rDNA sequencing revealed bacteria strains Bradyrhizobium japonicum (S12), Bradyrhizobium subterraneum (S1, S7, S8, S10, S13), Bradyrhizobium yuanmingense (S2, S3, S4, S6, S9, S11) while two bacteria were the endophytes identified as Pseudomonas azotoformans (S5) and Paenibacillus seodonensis (S15). Genetic diversity using UPGMA clustering revealed remarkable genetic variations, classifying 14 strains into three primary clusters and four secondary clusters. Strains S1, S3, S6, S9, and S13 exhibited elevated nitrogenase activity, indicative of their proficient nitrogen fixation capability. In plant growth experiments, all strains demonstrated significant growth of the plants compared to control. The notable growth enhancement by these five strains can be attributed to their remarkable efficiency in nitrogen fixation, as indicated by significantly higher nodule formation and nitrogenase activity. These findings provide valuable insights for identifying potential rhizobial inoculants to enhance pigeonpea productivity.
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| first_indexed | 2024-03-11T19:13:22Z |
| format | Article |
| id | doaj.art-ad5c990aeb3f489da5a9965e114c9287 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-03-11T19:13:22Z |
| publishDate | 2023-10-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-ad5c990aeb3f489da5a9965e114c92872023-10-09T09:04:25ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192023-10-01931010.56093/ijas.v93i10.140808Genetic diversity assessment and selection of Bradyrhizobium strains for Inceptisols based on symbiotic performanceSANTOSH KUMAR0PREETI SINGH1SHIV CHARAN KUMAR2NITISH RANJAN PRAKASH3UMAKANT BANJARE4ARUN KUMAR PATEL5VISHAL TYAGI6RAMESH KUMAR SINGH7Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, IndiaBanaras Hindu University, Varanasi, Uttar Pradesh 221 005, IndiaICAR-National Bureau of Agriculturally Important Microorganisms, Mau, Uttar PradeshICAR-Central Soil Salinity Research Institute, Regional Research Station, Canning town, West BengalBanaras Hindu University, Varanasi, Uttar Pradesh 221 005, IndiaBanaras Hindu University, Varanasi, Uttar Pradesh 221 005, IndiaICAR-Indian Agricultural Research Institute, New DelhiBanaras Hindu University, Varanasi, Uttar Pradesh 221 005, India The symbiotic association of pigeonpea (Cajanus cajan (L.) Millsp.) with rhizobia plays a pivotal role in plants’ growth and development. The present study was carried out at Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh during 2018–2020 to explore the diversity of rhizobia present in the Cajanus cajan root under Inceptisols and identify an elite strain that exhibits exceptional plant growth promotion and of nitrogenase activity for efficient nitrogen fixation. The bacterial identification using 16s rDNA sequencing revealed bacteria strains Bradyrhizobium japonicum (S12), Bradyrhizobium subterraneum (S1, S7, S8, S10, S13), Bradyrhizobium yuanmingense (S2, S3, S4, S6, S9, S11) while two bacteria were the endophytes identified as Pseudomonas azotoformans (S5) and Paenibacillus seodonensis (S15). Genetic diversity using UPGMA clustering revealed remarkable genetic variations, classifying 14 strains into three primary clusters and four secondary clusters. Strains S1, S3, S6, S9, and S13 exhibited elevated nitrogenase activity, indicative of their proficient nitrogen fixation capability. In plant growth experiments, all strains demonstrated significant growth of the plants compared to control. The notable growth enhancement by these five strains can be attributed to their remarkable efficiency in nitrogen fixation, as indicated by significantly higher nodule formation and nitrogenase activity. These findings provide valuable insights for identifying potential rhizobial inoculants to enhance pigeonpea productivity. https://epubs.icar.org.in/index.php/IJAgS/article/view/140808Bradyrhizobium, Cajanus cajan, Endophytes, Genetic diversity, Nitrogen fixation |
| spellingShingle | SANTOSH KUMAR PREETI SINGH SHIV CHARAN KUMAR NITISH RANJAN PRAKASH UMAKANT BANJARE ARUN KUMAR PATEL VISHAL TYAGI RAMESH KUMAR SINGH Genetic diversity assessment and selection of Bradyrhizobium strains for Inceptisols based on symbiotic performance The Indian Journal of Agricultural Sciences Bradyrhizobium, Cajanus cajan, Endophytes, Genetic diversity, Nitrogen fixation |
| title | Genetic diversity assessment and selection of Bradyrhizobium strains for Inceptisols based on symbiotic performance |
| title_full | Genetic diversity assessment and selection of Bradyrhizobium strains for Inceptisols based on symbiotic performance |
| title_fullStr | Genetic diversity assessment and selection of Bradyrhizobium strains for Inceptisols based on symbiotic performance |
| title_full_unstemmed | Genetic diversity assessment and selection of Bradyrhizobium strains for Inceptisols based on symbiotic performance |
| title_short | Genetic diversity assessment and selection of Bradyrhizobium strains for Inceptisols based on symbiotic performance |
| title_sort | genetic diversity assessment and selection of bradyrhizobium strains for inceptisols based on symbiotic performance |
| topic | Bradyrhizobium, Cajanus cajan, Endophytes, Genetic diversity, Nitrogen fixation |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/140808 |
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