Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insight
BackgroundAttention deficit hyperactivity disorder (ADHD) is characterized by impairments in developmental–behavioral inhibition, resulting in impulsivity and hyperactivity. Recent research has underscored cortical inhibition deficiencies in ADHD via the gamma-aminobutyric acid (GABA)ergic system, w...
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Psychiatry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpsyt.2024.1359237/full |
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| author | Ewelina Bogdańska-Chomczyk Maciej Równiak Andrew Chih-Wei Huang Anna Kozłowska |
| author_facet | Ewelina Bogdańska-Chomczyk Maciej Równiak Andrew Chih-Wei Huang Anna Kozłowska |
| author_sort | Ewelina Bogdańska-Chomczyk |
| collection | DOAJ |
| description | BackgroundAttention deficit hyperactivity disorder (ADHD) is characterized by impairments in developmental–behavioral inhibition, resulting in impulsivity and hyperactivity. Recent research has underscored cortical inhibition deficiencies in ADHD via the gamma-aminobutyric acid (GABA)ergic system, which is crucial for maintaining excitatory–inhibitory balance in the brain. This study explored postnatal changes in parvalbumin (PV) immunoreactivity, indicating GABAergic interneuron types, in the prefrontal (PFC) and motor (MC) cortices of spontaneously hypertensive rats (SHRs), an ADHD animal model.MethodsExamining PV- positive (PV+) cells associated with dopamine D2 receptors (D2) and the impact of dopamine on GABA synthesis, we also investigated changes in the immunoreactivity of D2 and tyrosine hydroxylase (TH). Brain sections from 4- to 10-week-old SHRs and Wistar Kyoto rats (WKYs) were immunohistochemically analyzed, comparing PV+, D2+ cells, and TH+ fiber densities across age-matched SHRs and WKYs in specific PFC/MC regions.ResultsThe results revealed significantly reduced PV+ cell density in SHRs: prelimbic (~20% less), anterior cingulate (~15% less), primary (~15% less), and secondary motor (~17% less) cortices. PV+ deficits coincided with the upregulation of D2 in prepubertal SHRs and the downregulation of TH predominantly in pubertal/postpubertal SHRs.ConclusionReduced PV+ cells in various PFC regions could contribute to inattention/behavioral alterations in ADHD, while MC deficits could manifest as motor hyperactivity. D2 upregulation and TH deficits may impact GABA synthesis, exacerbating behavioral deficits in ADHD. These findings not only shed new light on ADHD pathophysiology but also pave the way for future research endeavors. |
| first_indexed | 2024-04-24T18:17:13Z |
| format | Article |
| id | doaj.art-ad5f5ad0355c4c5788bd10e54581c9fa |
| institution | Directory Open Access Journal |
| issn | 1664-0640 |
| language | English |
| last_indexed | 2024-04-24T18:17:13Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Psychiatry |
| spelling | doaj.art-ad5f5ad0355c4c5788bd10e54581c9fa2024-03-27T13:41:36ZengFrontiers Media S.A.Frontiers in Psychiatry1664-06402024-03-011510.3389/fpsyt.2024.13592371359237Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insightEwelina Bogdańska-Chomczyk0Maciej Równiak1Andrew Chih-Wei Huang2Anna Kozłowska3Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury, Olsztyn, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Olsztyn, PolandDepartment of Psychology, Fo Guang University, Yilan County, TaiwanDepartment of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury, Olsztyn, PolandBackgroundAttention deficit hyperactivity disorder (ADHD) is characterized by impairments in developmental–behavioral inhibition, resulting in impulsivity and hyperactivity. Recent research has underscored cortical inhibition deficiencies in ADHD via the gamma-aminobutyric acid (GABA)ergic system, which is crucial for maintaining excitatory–inhibitory balance in the brain. This study explored postnatal changes in parvalbumin (PV) immunoreactivity, indicating GABAergic interneuron types, in the prefrontal (PFC) and motor (MC) cortices of spontaneously hypertensive rats (SHRs), an ADHD animal model.MethodsExamining PV- positive (PV+) cells associated with dopamine D2 receptors (D2) and the impact of dopamine on GABA synthesis, we also investigated changes in the immunoreactivity of D2 and tyrosine hydroxylase (TH). Brain sections from 4- to 10-week-old SHRs and Wistar Kyoto rats (WKYs) were immunohistochemically analyzed, comparing PV+, D2+ cells, and TH+ fiber densities across age-matched SHRs and WKYs in specific PFC/MC regions.ResultsThe results revealed significantly reduced PV+ cell density in SHRs: prelimbic (~20% less), anterior cingulate (~15% less), primary (~15% less), and secondary motor (~17% less) cortices. PV+ deficits coincided with the upregulation of D2 in prepubertal SHRs and the downregulation of TH predominantly in pubertal/postpubertal SHRs.ConclusionReduced PV+ cells in various PFC regions could contribute to inattention/behavioral alterations in ADHD, while MC deficits could manifest as motor hyperactivity. D2 upregulation and TH deficits may impact GABA synthesis, exacerbating behavioral deficits in ADHD. These findings not only shed new light on ADHD pathophysiology but also pave the way for future research endeavors.https://www.frontiersin.org/articles/10.3389/fpsyt.2024.1359237/fullattention-deficit/hyperactivity disordercortexparvalbumindopamine receptortyrosine hydroxylasespontaneously hypertensive rat |
| spellingShingle | Ewelina Bogdańska-Chomczyk Maciej Równiak Andrew Chih-Wei Huang Anna Kozłowska Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insight Frontiers in Psychiatry attention-deficit/hyperactivity disorder cortex parvalbumin dopamine receptor tyrosine hydroxylase spontaneously hypertensive rat |
| title | Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insight |
| title_full | Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insight |
| title_fullStr | Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insight |
| title_full_unstemmed | Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insight |
| title_short | Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insight |
| title_sort | parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats an attention deficit hyperactivity disorder animal model insight |
| topic | attention-deficit/hyperactivity disorder cortex parvalbumin dopamine receptor tyrosine hydroxylase spontaneously hypertensive rat |
| url | https://www.frontiersin.org/articles/10.3389/fpsyt.2024.1359237/full |
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