Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection
In this study, a novel label-free immunosensor platform is developed to exploit the localized surface plasmon resonance (LSPR) phenomenon. The LSPR solution-based platform is designed by a gold nanospheres probe, functionalized with monoclonal anti-dengue antibody (IgG). Numerical calculations are p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/11/11/1340 |
| _version_ | 1797510745896779776 |
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| author | Hafiz Zeeshan Mahmood Asim Jilani Sajid Farooq Yasir Javed Yasir Jamil Javed Iqbal Sami Ullah Swelm Wageh |
| author_facet | Hafiz Zeeshan Mahmood Asim Jilani Sajid Farooq Yasir Javed Yasir Jamil Javed Iqbal Sami Ullah Swelm Wageh |
| author_sort | Hafiz Zeeshan Mahmood |
| collection | DOAJ |
| description | In this study, a novel label-free immunosensor platform is developed to exploit the localized surface plasmon resonance (LSPR) phenomenon. The LSPR solution-based platform is designed by a gold nanospheres probe, functionalized with monoclonal anti-dengue antibody (IgG). Numerical calculations are performed to assess the LSPR extinction spectrum and spatial near electric field distribution around the nanoparticle surface. Important parameters that govern sensor performance, molecular and refractive index sensitivity are evaluated. On the evaluation of the platform as a molecular sensor, the detection of dengue NS1 antigens is presented. The results are consistent with the numerical simulations, which depicts the system’s ability to identify dengue NS1 antigen concentrations as low as 0.07 ± 0.01 µg/mL, along with fosters its potential application in plasmonic sensing. |
| first_indexed | 2024-03-10T05:35:41Z |
| format | Article |
| id | doaj.art-ad61d227129648449b36e936392d755e |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-10T05:35:41Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-ad61d227129648449b36e936392d755e2023-11-22T22:58:09ZengMDPI AGCrystals2073-43522021-11-011111134010.3390/cryst11111340Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue DetectionHafiz Zeeshan Mahmood0Asim Jilani1Sajid Farooq2Yasir Javed3Yasir Jamil4Javed Iqbal5Sami Ullah6Swelm Wageh7Department of Electrical Emgineering and Systems, Universidade Federal de Pernambuco, Recife 50670-901, BrazilCenter of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi ArabiaEscola Politécnica de Pernambuco—POLI, Universidade de Pernambuco, Recife 50100-010, BrazilDepartment of Physics, University of Agriculture, Faisalabad 38040, PakistanDepartment of Physics, University of Agriculture, Faisalabad 38040, PakistanCenter of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Chemistry, Faculty of Science, Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha 61413, Saudi ArabiaDepartment of Physics, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaIn this study, a novel label-free immunosensor platform is developed to exploit the localized surface plasmon resonance (LSPR) phenomenon. The LSPR solution-based platform is designed by a gold nanospheres probe, functionalized with monoclonal anti-dengue antibody (IgG). Numerical calculations are performed to assess the LSPR extinction spectrum and spatial near electric field distribution around the nanoparticle surface. Important parameters that govern sensor performance, molecular and refractive index sensitivity are evaluated. On the evaluation of the platform as a molecular sensor, the detection of dengue NS1 antigens is presented. The results are consistent with the numerical simulations, which depicts the system’s ability to identify dengue NS1 antigen concentrations as low as 0.07 ± 0.01 µg/mL, along with fosters its potential application in plasmonic sensing.https://www.mdpi.com/2073-4352/11/11/1340localized surface plasmon resonancegold nanoparticleplasmonic sensordengue detection |
| spellingShingle | Hafiz Zeeshan Mahmood Asim Jilani Sajid Farooq Yasir Javed Yasir Jamil Javed Iqbal Sami Ullah Swelm Wageh Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection Crystals localized surface plasmon resonance gold nanoparticle plasmonic sensor dengue detection |
| title | Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection |
| title_full | Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection |
| title_fullStr | Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection |
| title_full_unstemmed | Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection |
| title_short | Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection |
| title_sort | plasmon based label free biosensor using gold nanosphere for dengue detection |
| topic | localized surface plasmon resonance gold nanoparticle plasmonic sensor dengue detection |
| url | https://www.mdpi.com/2073-4352/11/11/1340 |
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