Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers
Enhanced ultraviolet-B (UV-B) radiation promotes anthocyanin biosynthesis in leaves, flowers and fruits of plants. However, the effects and underlying mechanisms of enhanced UV-B radiation on the accumulation of anthocyanins in the tubers of potatoes (<i>Solanum tuberosum</i> L.) remain...
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MDPI AG
2023-12-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/12/621 |
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| author | Lingyan Cui Maoxing Li Xing Zhang Zongming Guo Kaifeng Li Yuhan Shi Qiong Wang Huachun Guo |
| author_facet | Lingyan Cui Maoxing Li Xing Zhang Zongming Guo Kaifeng Li Yuhan Shi Qiong Wang Huachun Guo |
| author_sort | Lingyan Cui |
| collection | DOAJ |
| description | Enhanced ultraviolet-B (UV-B) radiation promotes anthocyanin biosynthesis in leaves, flowers and fruits of plants. However, the effects and underlying mechanisms of enhanced UV-B radiation on the accumulation of anthocyanins in the tubers of potatoes (<i>Solanum tuberosum</i> L.) remain unclear. Herein, reciprocal grafting experiments were first conducted using colored and uncolored potatoes, demonstrating that the anthocyanins in potato tubers were synthesized in situ, and not transported from the leaves to the tubers. Furthermore, the enhanced UV-B radiation (2.5 kJ·m<sup>−2</sup>·d<sup>−1</sup>) on potato stems and leaves significantly increased the contents of total anthocyanin and monomeric pelargonidin and peonidin in the red-fleshed potato ‘21-1’ tubers, compared to the untreated control. A comparative transcriptomic analysis showed that there were 2139 differentially expressed genes (DEGs) under UV-B treatment in comparison to the control, including 1724 up-regulated and 415 down-regulated genes. The anthocyanin-related enzymatic genes in the tubers such as <i>PAL</i>, <i>C4H</i>, <i>4CL</i>, <i>CHS</i>, <i>CHI</i>, <i>F3H</i>, <i>F3’5’H</i>, <i>ANS</i>, <i>UFGTs</i>, and <i>GSTs</i> were up-regulated under UV-B treatment, except for a down-regulated <i>F3’H</i>. A known anthocyanin-related transcription factor <i>StbHLH1</i> also showed a significantly higher expression level under UV-B treatment. Moreover, six differentially expressed MYB transcription factors were remarkably correlated to almost all anthocyanin-related enzymatic genes. Additionally, a DEGs enrichment analysis suggested that jasmonic acid might be a potential UV-B signaling molecule involved in the UV-B-induced tuber biosynthesis of anthocyanin. These results indicated that enhanced UV-B radiation in potato stems and leaves induced anthocyanin accumulation in the tubers by regulating the enzymatic genes and transcription factors involved in anthocyanin biosynthesis. This study provides novel insights into the mechanisms of enhanced UV-B radiation that regulate the anthocyanin biosynthesis in potato tubers. |
| first_indexed | 2024-03-08T20:53:56Z |
| format | Article |
| id | doaj.art-ad622852e79d42f28dedcc5d950359c0 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-08T20:53:56Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-ad622852e79d42f28dedcc5d950359c02023-12-22T14:00:43ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-12-0145129943996010.3390/cimb45120621Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in TubersLingyan Cui0Maoxing Li1Xing Zhang2Zongming Guo3Kaifeng Li4Yuhan Shi5Qiong Wang6Huachun Guo7College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaTuber and Root Crops Research Institute, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaTuber and Root Crops Research Institute, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, ChinaEnhanced ultraviolet-B (UV-B) radiation promotes anthocyanin biosynthesis in leaves, flowers and fruits of plants. However, the effects and underlying mechanisms of enhanced UV-B radiation on the accumulation of anthocyanins in the tubers of potatoes (<i>Solanum tuberosum</i> L.) remain unclear. Herein, reciprocal grafting experiments were first conducted using colored and uncolored potatoes, demonstrating that the anthocyanins in potato tubers were synthesized in situ, and not transported from the leaves to the tubers. Furthermore, the enhanced UV-B radiation (2.5 kJ·m<sup>−2</sup>·d<sup>−1</sup>) on potato stems and leaves significantly increased the contents of total anthocyanin and monomeric pelargonidin and peonidin in the red-fleshed potato ‘21-1’ tubers, compared to the untreated control. A comparative transcriptomic analysis showed that there were 2139 differentially expressed genes (DEGs) under UV-B treatment in comparison to the control, including 1724 up-regulated and 415 down-regulated genes. The anthocyanin-related enzymatic genes in the tubers such as <i>PAL</i>, <i>C4H</i>, <i>4CL</i>, <i>CHS</i>, <i>CHI</i>, <i>F3H</i>, <i>F3’5’H</i>, <i>ANS</i>, <i>UFGTs</i>, and <i>GSTs</i> were up-regulated under UV-B treatment, except for a down-regulated <i>F3’H</i>. A known anthocyanin-related transcription factor <i>StbHLH1</i> also showed a significantly higher expression level under UV-B treatment. Moreover, six differentially expressed MYB transcription factors were remarkably correlated to almost all anthocyanin-related enzymatic genes. Additionally, a DEGs enrichment analysis suggested that jasmonic acid might be a potential UV-B signaling molecule involved in the UV-B-induced tuber biosynthesis of anthocyanin. These results indicated that enhanced UV-B radiation in potato stems and leaves induced anthocyanin accumulation in the tubers by regulating the enzymatic genes and transcription factors involved in anthocyanin biosynthesis. This study provides novel insights into the mechanisms of enhanced UV-B radiation that regulate the anthocyanin biosynthesis in potato tubers.https://www.mdpi.com/1467-3045/45/12/621UV-B radiationpotatoanthocyaninin situ synthesistranscriptome |
| spellingShingle | Lingyan Cui Maoxing Li Xing Zhang Zongming Guo Kaifeng Li Yuhan Shi Qiong Wang Huachun Guo Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers Current Issues in Molecular Biology UV-B radiation potato anthocyanin in situ synthesis transcriptome |
| title | Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers |
| title_full | Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers |
| title_fullStr | Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers |
| title_full_unstemmed | Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers |
| title_short | Enhanced UV-B Radiation in Potato Stems and Leaves Promotes the Accumulation of Anthocyanins in Tubers |
| title_sort | enhanced uv b radiation in potato stems and leaves promotes the accumulation of anthocyanins in tubers |
| topic | UV-B radiation potato anthocyanin in situ synthesis transcriptome |
| url | https://www.mdpi.com/1467-3045/45/12/621 |
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