Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>)

Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>)

Tubby-like proteins (TLPs) play important roles in plant growth and development and in responses to abiotic stress. However, TLPs in strawberry remain poorly studied. In this study, eight <i>TLP</i>s were identified in woodland strawberry (<i>Fragaria vesca</i> subspecies <...

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Bibliographic Details
Main Authors: Shuangtao Li, Guixia Wang, Linlin Chang, Rui Sun, Ruishuang Wu, Chuanfei Zhong, Yongshun Gao, Hongli Zhang, Lingzhi Wei, Yongqing Wei, Yuntao Zhang, Jing Dong, Jian Sun
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:International Journal of Molecular Sciences
Subjects:
tubby-like proteins
<i>Fragaria vesca</i>
abiotic stress
evolution pattern
expression analysis
Online Access:https://www.mdpi.com/1422-0067/23/19/11961
Description
Summary:Tubby-like proteins (TLPs) play important roles in plant growth and development and in responses to abiotic stress. However, TLPs in strawberry remain poorly studied. In this study, eight <i>TLP</i>s were identified in woodland strawberry (<i>Fragaria vesca</i> subspecies <i>vesca</i> ‘Ruegen’). Protein structure analysis revealed that the structure of FvTLPs is highly conserved, but evolutionary and gene structure analyses revealed that the evolutionary pattern of <i>FvTLP</i> family members differs from that of their orthologous genes in <i>Arabidopsis</i>, poplar, and apple. Subcellular localization assays revealed that FvTLPs were localized to the nucleus and plasma membrane. FvTLPs showed no transcriptional activity. Yeast two-hybrid assays revealed that FvTLPs interact with specific FvSKP1s. The expression patterns of <i>FvTLP</i>s in different tissues and under various abiotic stresses (salt, drought, cold, and heat) and hormone treatments (ABA (abscisic acid) and MeJA (methyl jasmonate)) were determined. The expression patterns of <i>FvTLP</i>s indicated that they play a role in regulating growth and development and responses to abiotic stress in <i>F. vesca</i>. The GUS (beta-glucuronidase) activity of <i>FvTLP1</i>pro::GUS plants in GUS activity assays increased under salt and drought stress and abscisic acid treatment. The results of this study provide new insights into the molecular mechanisms underlying the functions of TLPs.
ISSN:1661-6596
1422-0067

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