Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>)
Tubby-like proteins (TLPs) play important roles in plant growth and development and in responses to abiotic stress. However, TLPs in strawberry remain poorly studied. In this study, eight <i>TLP</i>s were identified in woodland strawberry (<i>Fragaria vesca</i> subspecies <...
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| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11961 |
| _version_ | 1797478785900085248 |
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| author | Shuangtao Li Guixia Wang Linlin Chang Rui Sun Ruishuang Wu Chuanfei Zhong Yongshun Gao Hongli Zhang Lingzhi Wei Yongqing Wei Yuntao Zhang Jing Dong Jian Sun |
| author_facet | Shuangtao Li Guixia Wang Linlin Chang Rui Sun Ruishuang Wu Chuanfei Zhong Yongshun Gao Hongli Zhang Lingzhi Wei Yongqing Wei Yuntao Zhang Jing Dong Jian Sun |
| author_sort | Shuangtao Li |
| collection | DOAJ |
| description | Tubby-like proteins (TLPs) play important roles in plant growth and development and in responses to abiotic stress. However, TLPs in strawberry remain poorly studied. In this study, eight <i>TLP</i>s were identified in woodland strawberry (<i>Fragaria vesca</i> subspecies <i>vesca</i> ‘Ruegen’). Protein structure analysis revealed that the structure of FvTLPs is highly conserved, but evolutionary and gene structure analyses revealed that the evolutionary pattern of <i>FvTLP</i> family members differs from that of their orthologous genes in <i>Arabidopsis</i>, poplar, and apple. Subcellular localization assays revealed that FvTLPs were localized to the nucleus and plasma membrane. FvTLPs showed no transcriptional activity. Yeast two-hybrid assays revealed that FvTLPs interact with specific FvSKP1s. The expression patterns of <i>FvTLP</i>s in different tissues and under various abiotic stresses (salt, drought, cold, and heat) and hormone treatments (ABA (abscisic acid) and MeJA (methyl jasmonate)) were determined. The expression patterns of <i>FvTLP</i>s indicated that they play a role in regulating growth and development and responses to abiotic stress in <i>F. vesca</i>. The GUS (beta-glucuronidase) activity of <i>FvTLP1</i>pro::GUS plants in GUS activity assays increased under salt and drought stress and abscisic acid treatment. The results of this study provide new insights into the molecular mechanisms underlying the functions of TLPs. |
| first_indexed | 2024-03-09T21:36:35Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:36:35Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ad62c4ff88da4cae8222a4511473b1c72023-11-23T20:42:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191196110.3390/ijms231911961Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>)Shuangtao Li0Guixia Wang1Linlin Chang2Rui Sun3Ruishuang Wu4Chuanfei Zhong5Yongshun Gao6Hongli Zhang7Lingzhi Wei8Yongqing Wei9Yuntao Zhang10Jing Dong11Jian Sun12Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaInstitute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, ChinaTubby-like proteins (TLPs) play important roles in plant growth and development and in responses to abiotic stress. However, TLPs in strawberry remain poorly studied. In this study, eight <i>TLP</i>s were identified in woodland strawberry (<i>Fragaria vesca</i> subspecies <i>vesca</i> ‘Ruegen’). Protein structure analysis revealed that the structure of FvTLPs is highly conserved, but evolutionary and gene structure analyses revealed that the evolutionary pattern of <i>FvTLP</i> family members differs from that of their orthologous genes in <i>Arabidopsis</i>, poplar, and apple. Subcellular localization assays revealed that FvTLPs were localized to the nucleus and plasma membrane. FvTLPs showed no transcriptional activity. Yeast two-hybrid assays revealed that FvTLPs interact with specific FvSKP1s. The expression patterns of <i>FvTLP</i>s in different tissues and under various abiotic stresses (salt, drought, cold, and heat) and hormone treatments (ABA (abscisic acid) and MeJA (methyl jasmonate)) were determined. The expression patterns of <i>FvTLP</i>s indicated that they play a role in regulating growth and development and responses to abiotic stress in <i>F. vesca</i>. The GUS (beta-glucuronidase) activity of <i>FvTLP1</i>pro::GUS plants in GUS activity assays increased under salt and drought stress and abscisic acid treatment. The results of this study provide new insights into the molecular mechanisms underlying the functions of TLPs.https://www.mdpi.com/1422-0067/23/19/11961tubby-like proteins<i>Fragaria vesca</i>abiotic stressevolution patternexpression analysis |
| spellingShingle | Shuangtao Li Guixia Wang Linlin Chang Rui Sun Ruishuang Wu Chuanfei Zhong Yongshun Gao Hongli Zhang Lingzhi Wei Yongqing Wei Yuntao Zhang Jing Dong Jian Sun Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>) International Journal of Molecular Sciences tubby-like proteins <i>Fragaria vesca</i> abiotic stress evolution pattern expression analysis |
| title | Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>) |
| title_full | Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>) |
| title_fullStr | Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>) |
| title_full_unstemmed | Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>) |
| title_short | Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (<i>Fragaria vesca</i>) |
| title_sort | genome wide identification characterization and expression analysis of tubby like protein tlp gene family members in woodland strawberry i fragaria vesca i |
| topic | tubby-like proteins <i>Fragaria vesca</i> abiotic stress evolution pattern expression analysis |
| url | https://www.mdpi.com/1422-0067/23/19/11961 |
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