Culture Positivity of Cerebrospinal Fluid by Automated Blood Culture System in Neonates: A Cross-sectional Study from Loni, Maharashtra, India
Introduction: Bacterial meningitis especially in neonates remains a major cause of mortality and long term health sequelae. There is a need for periodic review, since pathogens responsible for the meningitis vary with time and geography. Aim: To find the culture positivity of Cerebrospinal Fluid (C...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2022-01-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://www.jcdr.net/articles/PDF/15842/52513_CE[Ra1]_F[SH]_PF1(AG_KM)_PFA(AG_KM)_PN(KM).pdf |
| _version_ | 1811175417293307904 |
|---|---|
| author | Savita Baban Tajane Anagha Subhashchandra Vaidya Deepika Shivaji Bhalerao Roushani Sanjeev Gopalrao Kulkarni Vaibhav Vitthalrao Rajhans Anita Balakrishnan Nair |
| author_facet | Savita Baban Tajane Anagha Subhashchandra Vaidya Deepika Shivaji Bhalerao Roushani Sanjeev Gopalrao Kulkarni Vaibhav Vitthalrao Rajhans Anita Balakrishnan Nair |
| author_sort | Savita Baban Tajane |
| collection | DOAJ |
| description | Introduction: Bacterial meningitis especially in neonates remains a major cause of mortality and long term health sequelae. There is a need for periodic review, since pathogens responsible for the meningitis vary with time and geography.
Aim: To find the culture positivity of Cerebrospinal Fluid (CSF) specimens before and after installation of automated blood culture and identification system in suspected neonatal meningitis cases.
Materials and Methods: The present descriptive cross-sectional study was carried out during July 2020 to December 2020, for six months duration in Department of Microbiology, Rural medical college, (Pravara Institute of Medical Sciences-Deemed University), Loni, Maharashtra, India. All CSF specimens from neonates <28 days received in Department of Microbiology were included in the study. All CSF specimens collected in BacT/Alert bottle were incubated and further subjected to identification by Vitek 2 system. All CSF specimen smears, received were subjected to Gram staining and Ziehl Neelsen Staining. Descriptive statistics was used for result analysis.
Results: A total of 265 CSF specimens were received during the study period. Males (59%) outnumbered females (41%) in the present study. Bacterial growth by automated blood culture system (BacT/Alert 3D) was detected in 85 CSF specimen giving the culture positivity as 32.08%. The most common bacterial isolate was found to be Staphylococcus haemolyticus followed by Enterococcus species and Acinetobacter species. Also, an attempt was made to compare culture positivity results with results by conventional culture method before installation of automation which showed heightened results for culture positivity and diversity of clinical isolates.
Conclusion: Bacterial neonatal meningitis is a common entity and aetiological diagnosis is crucial in every healthcare setting. The present study describes the various aetiological agents isolated by automated blood culture system in neonatal CSF specimens. Retrospective comparison with conventional culture has shown promising results for automated system. Early isolation and definitive identification with drug sensitivity, has got massive impact in management of neonate, further in timely progression of child’s developmental milestones. |
| first_indexed | 2024-04-10T19:35:50Z |
| format | Article |
| id | doaj.art-ad683437b7bf4bf28dd2d051bf13e7c8 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-10T19:35:50Z |
| publishDate | 2022-01-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-ad683437b7bf4bf28dd2d051bf13e7c82023-01-30T05:46:00ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2022-01-01161DC06DC0910.7860/JCDR/2022/52513.15842Culture Positivity of Cerebrospinal Fluid by Automated Blood Culture System in Neonates: A Cross-sectional Study from Loni, Maharashtra, IndiaSavita Baban Tajane0Anagha Subhashchandra Vaidya1Deepika Shivaji Bhalerao2Roushani3Sanjeev Gopalrao Kulkarni4Vaibhav Vitthalrao Rajhans5Anita Balakrishnan Nair6Postgraduate Student, Department of Microbiology, Rural Medical College, (PIMS-DU), Loni, Maharashtra, India.Professor, Department of Microbiology, Rural Medical College, (PIMS-DU), Loni, Maharashtra, India.Professor, Department of Microbiology, Rural Medical College, (PIMS-DU), Loni, Maharashtra, India.Professor and Head, Department of Microbiology, Rural Medical College, (PIMS-DU), Loni, Maharashtra, India.Associate Professor, Department of Microbiology, Rural Medical College, (PIMS-DU), Loni, Maharashtra, India.Associate Professor, Department of Microbiology, Rural Medical College, (PIMS-DU), Loni, Maharashtra, India.Assistant Professor, Department of Microbiology, Rural Medical College, (PIMS-DU), Loni, Maharashtra, India.Introduction: Bacterial meningitis especially in neonates remains a major cause of mortality and long term health sequelae. There is a need for periodic review, since pathogens responsible for the meningitis vary with time and geography. Aim: To find the culture positivity of Cerebrospinal Fluid (CSF) specimens before and after installation of automated blood culture and identification system in suspected neonatal meningitis cases. Materials and Methods: The present descriptive cross-sectional study was carried out during July 2020 to December 2020, for six months duration in Department of Microbiology, Rural medical college, (Pravara Institute of Medical Sciences-Deemed University), Loni, Maharashtra, India. All CSF specimens from neonates <28 days received in Department of Microbiology were included in the study. All CSF specimens collected in BacT/Alert bottle were incubated and further subjected to identification by Vitek 2 system. All CSF specimen smears, received were subjected to Gram staining and Ziehl Neelsen Staining. Descriptive statistics was used for result analysis. Results: A total of 265 CSF specimens were received during the study period. Males (59%) outnumbered females (41%) in the present study. Bacterial growth by automated blood culture system (BacT/Alert 3D) was detected in 85 CSF specimen giving the culture positivity as 32.08%. The most common bacterial isolate was found to be Staphylococcus haemolyticus followed by Enterococcus species and Acinetobacter species. Also, an attempt was made to compare culture positivity results with results by conventional culture method before installation of automation which showed heightened results for culture positivity and diversity of clinical isolates. Conclusion: Bacterial neonatal meningitis is a common entity and aetiological diagnosis is crucial in every healthcare setting. The present study describes the various aetiological agents isolated by automated blood culture system in neonatal CSF specimens. Retrospective comparison with conventional culture has shown promising results for automated system. Early isolation and definitive identification with drug sensitivity, has got massive impact in management of neonate, further in timely progression of child’s developmental milestones.https://www.jcdr.net/articles/PDF/15842/52513_CE[Ra1]_F[SH]_PF1(AG_KM)_PFA(AG_KM)_PN(KM).pdfcerebrospinal fluid culturegram positive coccineonatal meningitis |
| spellingShingle | Savita Baban Tajane Anagha Subhashchandra Vaidya Deepika Shivaji Bhalerao Roushani Sanjeev Gopalrao Kulkarni Vaibhav Vitthalrao Rajhans Anita Balakrishnan Nair Culture Positivity of Cerebrospinal Fluid by Automated Blood Culture System in Neonates: A Cross-sectional Study from Loni, Maharashtra, India Journal of Clinical and Diagnostic Research cerebrospinal fluid culture gram positive cocci neonatal meningitis |
| title | Culture Positivity of Cerebrospinal Fluid by Automated Blood Culture System in Neonates: A Cross-sectional Study from Loni, Maharashtra, India |
| title_full | Culture Positivity of Cerebrospinal Fluid by Automated Blood Culture System in Neonates: A Cross-sectional Study from Loni, Maharashtra, India |
| title_fullStr | Culture Positivity of Cerebrospinal Fluid by Automated Blood Culture System in Neonates: A Cross-sectional Study from Loni, Maharashtra, India |
| title_full_unstemmed | Culture Positivity of Cerebrospinal Fluid by Automated Blood Culture System in Neonates: A Cross-sectional Study from Loni, Maharashtra, India |
| title_short | Culture Positivity of Cerebrospinal Fluid by Automated Blood Culture System in Neonates: A Cross-sectional Study from Loni, Maharashtra, India |
| title_sort | culture positivity of cerebrospinal fluid by automated blood culture system in neonates a cross sectional study from loni maharashtra india |
| topic | cerebrospinal fluid culture gram positive cocci neonatal meningitis |
| url | https://www.jcdr.net/articles/PDF/15842/52513_CE[Ra1]_F[SH]_PF1(AG_KM)_PFA(AG_KM)_PN(KM).pdf |
| work_keys_str_mv | AT savitababantajane culturepositivityofcerebrospinalfluidbyautomatedbloodculturesysteminneonatesacrosssectionalstudyfromlonimaharashtraindia AT anaghasubhashchandravaidya culturepositivityofcerebrospinalfluidbyautomatedbloodculturesysteminneonatesacrosssectionalstudyfromlonimaharashtraindia AT deepikashivajibhalerao culturepositivityofcerebrospinalfluidbyautomatedbloodculturesysteminneonatesacrosssectionalstudyfromlonimaharashtraindia AT roushani culturepositivityofcerebrospinalfluidbyautomatedbloodculturesysteminneonatesacrosssectionalstudyfromlonimaharashtraindia AT sanjeevgopalraokulkarni culturepositivityofcerebrospinalfluidbyautomatedbloodculturesysteminneonatesacrosssectionalstudyfromlonimaharashtraindia AT vaibhavvitthalraorajhans culturepositivityofcerebrospinalfluidbyautomatedbloodculturesysteminneonatesacrosssectionalstudyfromlonimaharashtraindia AT anitabalakrishnannair culturepositivityofcerebrospinalfluidbyautomatedbloodculturesysteminneonatesacrosssectionalstudyfromlonimaharashtraindia |