Target identification of small molecules: an overview of the current applications in drug discovery
Abstract Target identification is an essential part of the drug discovery and development process, and its efficacy plays a crucial role in the success of any given therapy. Although protein target identification research can be challenging, two main approaches can help researchers make significant...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-10-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12896-023-00815-4 |
| _version_ | 1797452187856535552 |
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| author | Yasser Tabana Dinesh Babu Richard Fahlman Arno G. Siraki Khaled Barakat |
| author_facet | Yasser Tabana Dinesh Babu Richard Fahlman Arno G. Siraki Khaled Barakat |
| author_sort | Yasser Tabana |
| collection | DOAJ |
| description | Abstract Target identification is an essential part of the drug discovery and development process, and its efficacy plays a crucial role in the success of any given therapy. Although protein target identification research can be challenging, two main approaches can help researchers make significant discoveries: affinity-based pull-down and label-free methods. Affinity-based pull-down methods use small molecules conjugated with tags to selectively isolate target proteins, while label-free methods utilize small molecules in their natural state to identify targets. Target identification strategy selection is essential to the success of any drug discovery process and must be carefully considered when determining how to best pursue a specific project. This paper provides an overview of the current target identification approaches in drug discovery related to experimental biological assays, focusing primarily on affinity-based pull-down and label-free approaches, and discusses their main limitations and advantages. |
| first_indexed | 2024-03-09T15:04:14Z |
| format | Article |
| id | doaj.art-ad68db5e05614a6bb9e27cef6e481e96 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-03-09T15:04:14Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-ad68db5e05614a6bb9e27cef6e481e962023-11-26T13:44:01ZengBMCBMC Biotechnology1472-67502023-10-0123111910.1186/s12896-023-00815-4Target identification of small molecules: an overview of the current applications in drug discoveryYasser Tabana0Dinesh Babu1Richard Fahlman2Arno G. Siraki3Khaled Barakat4Faculty of Pharmacy and Pharmaceutical Sciences, University of AlbertaLi Ka Shing Applied Virology Institute, University of AlbertaDepartment of Biochemistry, University of AlbertaFaculty of Pharmacy and Pharmaceutical Sciences, University of AlbertaFaculty of Pharmacy and Pharmaceutical Sciences, University of AlbertaAbstract Target identification is an essential part of the drug discovery and development process, and its efficacy plays a crucial role in the success of any given therapy. Although protein target identification research can be challenging, two main approaches can help researchers make significant discoveries: affinity-based pull-down and label-free methods. Affinity-based pull-down methods use small molecules conjugated with tags to selectively isolate target proteins, while label-free methods utilize small molecules in their natural state to identify targets. Target identification strategy selection is essential to the success of any drug discovery process and must be carefully considered when determining how to best pursue a specific project. This paper provides an overview of the current target identification approaches in drug discovery related to experimental biological assays, focusing primarily on affinity-based pull-down and label-free approaches, and discusses their main limitations and advantages.https://doi.org/10.1186/s12896-023-00815-4Small moleculeTarget identificationAffinity-based pull-downLabel-free |
| spellingShingle | Yasser Tabana Dinesh Babu Richard Fahlman Arno G. Siraki Khaled Barakat Target identification of small molecules: an overview of the current applications in drug discovery BMC Biotechnology Small molecule Target identification Affinity-based pull-down Label-free |
| title | Target identification of small molecules: an overview of the current applications in drug discovery |
| title_full | Target identification of small molecules: an overview of the current applications in drug discovery |
| title_fullStr | Target identification of small molecules: an overview of the current applications in drug discovery |
| title_full_unstemmed | Target identification of small molecules: an overview of the current applications in drug discovery |
| title_short | Target identification of small molecules: an overview of the current applications in drug discovery |
| title_sort | target identification of small molecules an overview of the current applications in drug discovery |
| topic | Small molecule Target identification Affinity-based pull-down Label-free |
| url | https://doi.org/10.1186/s12896-023-00815-4 |
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