Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients.
Diagnosis of visceral leishmaniasis (VL) relies on invasive and risky aspirate procedures, and confirmation of cure after treatment is unreliable. Detection of Leishmania donovani antigens in urine has the potential to provide both a non-invasive diagnostic and a test of cure. We searched for L. don...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0238840 |
| _version_ | 1830188614506512384 |
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| author | Tegwen Marlais Tapan Bhattacharyya Callum Pearson Bathsheba L Gardner Safiyyah Marhoon Stephanie Airs Kiera Hayes Andrew K Falconar Om Prakash Singh Steven G Reed Sayda El-Safi Shyam Sundar Michael A Miles |
| author_facet | Tegwen Marlais Tapan Bhattacharyya Callum Pearson Bathsheba L Gardner Safiyyah Marhoon Stephanie Airs Kiera Hayes Andrew K Falconar Om Prakash Singh Steven G Reed Sayda El-Safi Shyam Sundar Michael A Miles |
| author_sort | Tegwen Marlais |
| collection | DOAJ |
| description | Diagnosis of visceral leishmaniasis (VL) relies on invasive and risky aspirate procedures, and confirmation of cure after treatment is unreliable. Detection of Leishmania donovani antigens in urine has the potential to provide both a non-invasive diagnostic and a test of cure. We searched for L. donovani antigens in urine of VL patients from India and Sudan to contribute to the development of urine antigen capture immunoassays. VL urine samples were incubated with immobilised anti-L. donovani polyclonal antibodies and captured material was eluted. Sudanese eluted material and concentrated VL urine were analysed by western blot. Immunocaptured and immunoreactive material from Indian and Sudanese urine was submitted to mass spectrometry for protein identification. We identified six L. donovani proteins from VL urine. Named proteins were 40S ribosomal protein S9, kinases, and others were hypothetical. Thirty-three epitope regions were predicted with high specificity in the 6 proteins. Of these, 20 were highly specific to Leishmania spp. and are highly suitable for raising antibodies for the subsequent development of an antigen capture assay. We present all the identified proteins and analysed epitope regions in full so that they may contribute to the development of non-invasive immunoassays for this deadly disease. |
| first_indexed | 2024-12-17T22:48:53Z |
| format | Article |
| id | doaj.art-ad6c1be4829b439387e5d0b9f3086143 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T22:48:53Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ad6c1be4829b439387e5d0b9f30861432022-12-21T21:29:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01159e023884010.1371/journal.pone.0238840Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients.Tegwen MarlaisTapan BhattacharyyaCallum PearsonBathsheba L GardnerSafiyyah MarhoonStephanie AirsKiera HayesAndrew K FalconarOm Prakash SinghSteven G ReedSayda El-SafiShyam SundarMichael A MilesDiagnosis of visceral leishmaniasis (VL) relies on invasive and risky aspirate procedures, and confirmation of cure after treatment is unreliable. Detection of Leishmania donovani antigens in urine has the potential to provide both a non-invasive diagnostic and a test of cure. We searched for L. donovani antigens in urine of VL patients from India and Sudan to contribute to the development of urine antigen capture immunoassays. VL urine samples were incubated with immobilised anti-L. donovani polyclonal antibodies and captured material was eluted. Sudanese eluted material and concentrated VL urine were analysed by western blot. Immunocaptured and immunoreactive material from Indian and Sudanese urine was submitted to mass spectrometry for protein identification. We identified six L. donovani proteins from VL urine. Named proteins were 40S ribosomal protein S9, kinases, and others were hypothetical. Thirty-three epitope regions were predicted with high specificity in the 6 proteins. Of these, 20 were highly specific to Leishmania spp. and are highly suitable for raising antibodies for the subsequent development of an antigen capture assay. We present all the identified proteins and analysed epitope regions in full so that they may contribute to the development of non-invasive immunoassays for this deadly disease.https://doi.org/10.1371/journal.pone.0238840 |
| spellingShingle | Tegwen Marlais Tapan Bhattacharyya Callum Pearson Bathsheba L Gardner Safiyyah Marhoon Stephanie Airs Kiera Hayes Andrew K Falconar Om Prakash Singh Steven G Reed Sayda El-Safi Shyam Sundar Michael A Miles Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients. PLoS ONE |
| title | Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients. |
| title_full | Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients. |
| title_fullStr | Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients. |
| title_full_unstemmed | Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients. |
| title_short | Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients. |
| title_sort | isolation and characterisation of leishmania donovani protein antigens from urine of visceral leishmaniasis patients |
| url | https://doi.org/10.1371/journal.pone.0238840 |
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