Assessment of The Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) with Different Methods
The aim of this prospective study was to determine the prevalence of methicillin resistant Staphylococcus aureus that were isolated from various clinical specimens in our hospital and to search for methicillin resistance by using different methods. Hundred and seventeen isolates were studied. Of the...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2001-09-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2001-6-3-178-183.pdf |
| _version_ | 1797910722390261760 |
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| author | Vildan AVKAN OĞUZ Senay DODANLI İpek YILDIRIM Oğuz ÖZTÜRK Nevin SARIGÜZEL Engin SEBER |
| author_facet | Vildan AVKAN OĞUZ Senay DODANLI İpek YILDIRIM Oğuz ÖZTÜRK Nevin SARIGÜZEL Engin SEBER |
| author_sort | Vildan AVKAN OĞUZ |
| collection | DOAJ |
| description | The aim of this prospective study was to determine the prevalence of methicillin resistant Staphylococcus aureus that were isolated from various clinical specimens in our hospital and to search for methicillin resistance by using different methods. Hundred and seventeen isolates were studied. Of these, 41(35%) were found to be resistant to methicillin with agar dilution; 39 (33.3%) with agar screening and 38 (32.5%) with disk diffusion. Of the resistant strains, 27 (65.8%) were found to be positive both for beta-lactamase and mec A; while 3 (7.4%) only for mec A; 6 (14.6%) only for beta-lactamase. Neither beta-lactamase nor mec A were determined in 5 (12.2%) strains. As a conclusion we have found no statistically significant difference in sensitivity and specificity between agar screening and disk diffusion methods used in the detection of methicillin resistance (Mc Nemar and Cohen Kappa statistical tests were used in the study). Yet we recommend to retest the isolates which are found to be methicillin resistant by disk diffusion method used routinely, with agar screening also which is cheaper and more simple. Than dilution tests this test can give additional information about MIC levels. Furthermore exploring for mec A gene in these isolates contributes more in therapeutic approach. |
| first_indexed | 2024-04-10T11:30:13Z |
| format | Article |
| id | doaj.art-ad6cbce47be2449aa456d98e6c103f6a |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T11:30:13Z |
| publishDate | 2001-09-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-ad6cbce47be2449aa456d98e6c103f6a2023-02-15T16:18:10ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2001-09-0163178183Assessment of The Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) with Different MethodsVildan AVKAN OĞUZ0Senay DODANLI1İpek YILDIRIM2Oğuz ÖZTÜRK3Nevin SARIGÜZEL4Engin SEBER5Şişli Etfal Hastanesi, İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği,Şişli Etfal Hastanesi, İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği,Şişli Etfal Hastanesi, İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği,İstanbul Üniversitesi, Deneysel Tıp Araştırma Enstitüsü (DETAE) Moleküler Tıp Anabilim Dalı, İSTANBULŞişli Etfal Hastanesi, İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği,Şişli Etfal Hastanesi, İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği,The aim of this prospective study was to determine the prevalence of methicillin resistant Staphylococcus aureus that were isolated from various clinical specimens in our hospital and to search for methicillin resistance by using different methods. Hundred and seventeen isolates were studied. Of these, 41(35%) were found to be resistant to methicillin with agar dilution; 39 (33.3%) with agar screening and 38 (32.5%) with disk diffusion. Of the resistant strains, 27 (65.8%) were found to be positive both for beta-lactamase and mec A; while 3 (7.4%) only for mec A; 6 (14.6%) only for beta-lactamase. Neither beta-lactamase nor mec A were determined in 5 (12.2%) strains. As a conclusion we have found no statistically significant difference in sensitivity and specificity between agar screening and disk diffusion methods used in the detection of methicillin resistance (Mc Nemar and Cohen Kappa statistical tests were used in the study). Yet we recommend to retest the isolates which are found to be methicillin resistant by disk diffusion method used routinely, with agar screening also which is cheaper and more simple. Than dilution tests this test can give additional information about MIC levels. Furthermore exploring for mec A gene in these isolates contributes more in therapeutic approach.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2001-6-3-178-183.pdfStaphylococcus aureusAgar dilutionAgar plate screening (agar spread method)Disk diffusionBeta-lactamasePolymerase chain reactionMec A |
| spellingShingle | Vildan AVKAN OĞUZ Senay DODANLI İpek YILDIRIM Oğuz ÖZTÜRK Nevin SARIGÜZEL Engin SEBER Assessment of The Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) with Different Methods Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Staphylococcus aureus Agar dilution Agar plate screening (agar spread method) Disk diffusion Beta-lactamase Polymerase chain reaction Mec A |
| title | Assessment of The Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) with Different Methods |
| title_full | Assessment of The Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) with Different Methods |
| title_fullStr | Assessment of The Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) with Different Methods |
| title_full_unstemmed | Assessment of The Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) with Different Methods |
| title_short | Assessment of The Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) with Different Methods |
| title_sort | assessment of the prevalence of methicillin resistant staphylococcus aureus mrsa with different methods |
| topic | Staphylococcus aureus Agar dilution Agar plate screening (agar spread method) Disk diffusion Beta-lactamase Polymerase chain reaction Mec A |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2001-6-3-178-183.pdf |
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