RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonist
Bacterial small RNAs (sRNAs) are important post-transcriptional regulators in stress responses and virulence. They can be derived from an expanding list of genomic contexts, such as processing from parental transcripts by RNase E. The role of RNase III in sRNA biogenesis is less well understood desp...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/69064 |
| _version_ | 1811227366617251840 |
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| author | Sarah Lauren Svensson Cynthia Mira Sharma |
| author_facet | Sarah Lauren Svensson Cynthia Mira Sharma |
| author_sort | Sarah Lauren Svensson |
| collection | DOAJ |
| description | Bacterial small RNAs (sRNAs) are important post-transcriptional regulators in stress responses and virulence. They can be derived from an expanding list of genomic contexts, such as processing from parental transcripts by RNase E. The role of RNase III in sRNA biogenesis is less well understood despite its well-known roles in rRNA processing, RNA decay, and cleavage of sRNA-mRNA duplexes. Here, we show that RNase III processes a pair of cis-encoded sRNAs (CJnc190 and CJnc180) of the food-borne pathogen Campylobacter jejuni. While CJnc180 processing by RNase III requires CJnc190, RNase III processes CJnc190 independent of CJnc180 via cleavage of an intramolecular duplex. We also show that CJnc190 directly represses translation of the colonization factor PtmG by targeting a G-rich ribosome-binding site, and uncover that CJnc180 is a cis-acting antagonist of CJnc190, indirectly affecting ptmG regulation. Our study highlights a role for RNase III in sRNA biogenesis and adds cis-encoded RNAs to the expanding diversity of transcripts that can antagonize bacterial sRNAs. |
| first_indexed | 2024-04-12T09:41:18Z |
| format | Article |
| id | doaj.art-ad706a655dc64487be5e028a311e089e |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:41:18Z |
| publishDate | 2021-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ad706a655dc64487be5e028a311e089e2022-12-22T03:38:04ZengeLife Sciences Publications LtdeLife2050-084X2021-11-011010.7554/eLife.69064RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonistSarah Lauren Svensson0https://orcid.org/0000-0002-3183-6084Cynthia Mira Sharma1https://orcid.org/0000-0002-2321-9705Department of Molecular Infection Biology II, Institute of Molecular Infection Biology, University of Würzburg, Würzburg, GermanyDepartment of Molecular Infection Biology II, Institute of Molecular Infection Biology, University of Würzburg, Würzburg, GermanyBacterial small RNAs (sRNAs) are important post-transcriptional regulators in stress responses and virulence. They can be derived from an expanding list of genomic contexts, such as processing from parental transcripts by RNase E. The role of RNase III in sRNA biogenesis is less well understood despite its well-known roles in rRNA processing, RNA decay, and cleavage of sRNA-mRNA duplexes. Here, we show that RNase III processes a pair of cis-encoded sRNAs (CJnc190 and CJnc180) of the food-borne pathogen Campylobacter jejuni. While CJnc180 processing by RNase III requires CJnc190, RNase III processes CJnc190 independent of CJnc180 via cleavage of an intramolecular duplex. We also show that CJnc190 directly represses translation of the colonization factor PtmG by targeting a G-rich ribosome-binding site, and uncover that CJnc180 is a cis-acting antagonist of CJnc190, indirectly affecting ptmG regulation. Our study highlights a role for RNase III in sRNA biogenesis and adds cis-encoded RNAs to the expanding diversity of transcripts that can antagonize bacterial sRNAs.https://elifesciences.org/articles/69064Campylobacter jejunismall regulatory RNAsRNARNase IIIpost-transcriptional regulationpathogenesis |
| spellingShingle | Sarah Lauren Svensson Cynthia Mira Sharma RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonist eLife Campylobacter jejuni small regulatory RNA sRNA RNase III post-transcriptional regulation pathogenesis |
| title | RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonist |
| title_full | RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonist |
| title_fullStr | RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonist |
| title_full_unstemmed | RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonist |
| title_short | RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonist |
| title_sort | rnase iii mediated processing of a trans acting bacterial srna and its cis encoded antagonist |
| topic | Campylobacter jejuni small regulatory RNA sRNA RNase III post-transcriptional regulation pathogenesis |
| url | https://elifesciences.org/articles/69064 |
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