Achillea mellifolium ethanolic extract Protective effects on ventral horn of the spinal cord alpha motoneurons degeneration after sciatic nerve compression in rats
Background and Aim: There are several reports regarding anti-inflammatory and tissue repair properties of the plant Achillea, but neuroprotective role of ethanolic extract of Achillea millefolium has not been studied after peripheral nerve injury. Therefore, the purpose of the present study was to a...
| Main Authors: | , |
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| Format: | Article |
| Language: | fas |
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Birjand University of Medical Sciences and Health Services
2015-12-01
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| Series: | مجله دانشگاه علوم پزشکی بیرجند |
| Subjects: | |
| Online Access: | http://journal.bums.ac.ir/browse.php?a_code=A-10-1185-2&slc_lang=en&sid=1 |
| _version_ | 1818554651733131264 |
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| author | Ali Shahraki Abbass Shahraki |
| author_facet | Ali Shahraki Abbass Shahraki |
| author_sort | Ali Shahraki |
| collection | DOAJ |
| description | Background and Aim: There are several reports regarding anti-inflammatory and tissue repair properties of the plant Achillea, but neuroprotective role of ethanolic extract of Achillea millefolium has not been studied after peripheral nerve injury. Therefore, the purpose of the present study was to assess neuroprotective effects .of Achillea millefolium ethanolic extract on the spinal cord alpha motor neuons after sciatic nerve compression in male rats.
Materials and Methods: In this experimental study 30 male Wistar rats each weighing 200-250g were chosen and were randomly divided into 5 equal groups including control, compressed, and three compressed groups plus intraperitoneal injection of Achillea millefolium ethanolic extract with the concentration of 50, 75, and 100 mg/kg; once a week for three weeks. . Sciatic nerve compression in these four groups . was done using hematostatic forceps for 60 seconds. After 28 days, L4, L5,S1, and S3 of the spinal cord were sampled using perfusion method. Statistical analysis of the obtained data was done by means of one-way Anova and Tukey post- hoc test using SPSS( version 19 ) at the significant level of P<0.05.
Results: It was found that α-motor neurons density in the compression group (666.6±39.17) significantly decreased compared to the control group (1754±34.22) ;P <0.001. Neural density in the groups treated with ethanolic extract, i.e. 50 mg/kg .,75 mg/kg, .and.100 mg/kg was 1236±69.72.,.1444.3±39.17,.and 1546.3±57.39 respectively ;which showed a significant increase compared to the compression group (P<0.01).
Conclusion: Ethanolic extract of Achillea millefolium had a neuroprotective effect after sciatic nerve compression. Presumably, this is due to antioxidant and anti-inflammatory compounds in the plant. |
| first_indexed | 2024-12-12T09:42:48Z |
| format | Article |
| id | doaj.art-ad74257c94114834899eb243a32e39eb |
| institution | Directory Open Access Journal |
| issn | 1607-2197 2423-6152 |
| language | fas |
| last_indexed | 2024-12-12T09:42:48Z |
| publishDate | 2015-12-01 |
| publisher | Birjand University of Medical Sciences and Health Services |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی بیرجند |
| spelling | doaj.art-ad74257c94114834899eb243a32e39eb2022-12-22T00:28:31ZfasBirjand University of Medical Sciences and Health Servicesمجله دانشگاه علوم پزشکی بیرجند1607-21972423-61522015-12-01224340348Achillea mellifolium ethanolic extract Protective effects on ventral horn of the spinal cord alpha motoneurons degeneration after sciatic nerve compression in ratsAli Shahraki0Abbass Shahraki1 Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran. Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran. Background and Aim: There are several reports regarding anti-inflammatory and tissue repair properties of the plant Achillea, but neuroprotective role of ethanolic extract of Achillea millefolium has not been studied after peripheral nerve injury. Therefore, the purpose of the present study was to assess neuroprotective effects .of Achillea millefolium ethanolic extract on the spinal cord alpha motor neuons after sciatic nerve compression in male rats. Materials and Methods: In this experimental study 30 male Wistar rats each weighing 200-250g were chosen and were randomly divided into 5 equal groups including control, compressed, and three compressed groups plus intraperitoneal injection of Achillea millefolium ethanolic extract with the concentration of 50, 75, and 100 mg/kg; once a week for three weeks. . Sciatic nerve compression in these four groups . was done using hematostatic forceps for 60 seconds. After 28 days, L4, L5,S1, and S3 of the spinal cord were sampled using perfusion method. Statistical analysis of the obtained data was done by means of one-way Anova and Tukey post- hoc test using SPSS( version 19 ) at the significant level of P<0.05. Results: It was found that α-motor neurons density in the compression group (666.6±39.17) significantly decreased compared to the control group (1754±34.22) ;P <0.001. Neural density in the groups treated with ethanolic extract, i.e. 50 mg/kg .,75 mg/kg, .and.100 mg/kg was 1236±69.72.,.1444.3±39.17,.and 1546.3±57.39 respectively ;which showed a significant increase compared to the compression group (P<0.01). Conclusion: Ethanolic extract of Achillea millefolium had a neuroprotective effect after sciatic nerve compression. Presumably, this is due to antioxidant and anti-inflammatory compounds in the plant.http://journal.bums.ac.ir/browse.php?a_code=A-10-1185-2&slc_lang=en&sid=1Achillea millefolium Ethanolic extract Neurodegeneration Neuroprotection |
| spellingShingle | Ali Shahraki Abbass Shahraki Achillea mellifolium ethanolic extract Protective effects on ventral horn of the spinal cord alpha motoneurons degeneration after sciatic nerve compression in rats مجله دانشگاه علوم پزشکی بیرجند Achillea millefolium Ethanolic extract Neurodegeneration Neuroprotection |
| title | Achillea mellifolium ethanolic extract Protective effects on ventral horn of the spinal cord alpha motoneurons degeneration after sciatic nerve compression in rats |
| title_full | Achillea mellifolium ethanolic extract Protective effects on ventral horn of the spinal cord alpha motoneurons degeneration after sciatic nerve compression in rats |
| title_fullStr | Achillea mellifolium ethanolic extract Protective effects on ventral horn of the spinal cord alpha motoneurons degeneration after sciatic nerve compression in rats |
| title_full_unstemmed | Achillea mellifolium ethanolic extract Protective effects on ventral horn of the spinal cord alpha motoneurons degeneration after sciatic nerve compression in rats |
| title_short | Achillea mellifolium ethanolic extract Protective effects on ventral horn of the spinal cord alpha motoneurons degeneration after sciatic nerve compression in rats |
| title_sort | achillea mellifolium ethanolic extract protective effects on ventral horn of the spinal cord alpha motoneurons degeneration after sciatic nerve compression in rats |
| topic | Achillea millefolium Ethanolic extract Neurodegeneration Neuroprotection |
| url | http://journal.bums.ac.ir/browse.php?a_code=A-10-1185-2&slc_lang=en&sid=1 |
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