Identification and Characterization of <i>Heme Oxygenase-1</i> from <i>Litopenaeus vannamei</i> Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress
Heme oxygenase-1 (HO-1) is a stress-inducible enzyme with antioxidant, anti-inflammatory, and anti-apoptotic effects. In this study, the <i>HO-1</i> gene from <i>Litopenaeus vannamei</i> (<i>Lv-HO-1</i>) was identified. The open reading frame of <i>Lv-HO-1&l...
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MDPI AG
2022-11-01
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| author | Yongxiong Huang Qi Li Shiping Yang Yunhao Yuan Zhiqiang Zhang Baijian Jiang Jing Lv Jian Zhong Jichang Jian |
| author_facet | Yongxiong Huang Qi Li Shiping Yang Yunhao Yuan Zhiqiang Zhang Baijian Jiang Jing Lv Jian Zhong Jichang Jian |
| author_sort | Yongxiong Huang |
| collection | DOAJ |
| description | Heme oxygenase-1 (HO-1) is a stress-inducible enzyme with antioxidant, anti-inflammatory, and anti-apoptotic effects. In this study, the <i>HO-1</i> gene from <i>Litopenaeus vannamei</i> (<i>Lv-HO-1</i>) was identified. The open reading frame of <i>Lv-HO-1</i> is 747 bp, encoding a peptide of 248 amino acids as well as a conserved HemO structural domain. Lv-HO-1 is 70–90% homological to crustaceans and about 50% homological to arthropods. The transcript levels of <i>Lv-HO-1</i> were highest in the hepatopancreas and lower in other tissues. Knockdown of <i>Lv-HO-1</i> led to structural destruction of the hepatopancreas. After ammonia exposure, <i>Lv-HO-1</i> was significantly induced. Knockdown of <i>Lv-HO-1</i> during ammonia exposure resulted in a significant decrease in antioxidant capacity and cellular autophagy levels compared to the control and increased apoptosis. The transcriptional levels of <i>SOD</i> and <i>GSH-Px</i> were considerably reduced (<i>p</i> < 0.05), as were the transcriptional levels of <i>Atg3</i>, <i>Atg4</i>, <i>Atg5</i>, and <i>Atg10</i>. The results indicated that <i>Lv-HO-1</i> from <i>L. vannamei</i> can be induced by oxidative stress and may have important roles in regulating the host antioxidant system, reducing cell apoptosis. |
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| last_indexed | 2024-03-09T16:44:16Z |
| publishDate | 2022-11-01 |
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| series | Fishes |
| spelling | doaj.art-ad74d6231b614b89ae3602bc5e6fc83b2023-11-24T14:48:20ZengMDPI AGFishes2410-38882022-11-017635610.3390/fishes7060356Identification and Characterization of <i>Heme Oxygenase-1</i> from <i>Litopenaeus vannamei</i> Involved in Antioxidant and Anti-Apoptosis under Ammonia StressYongxiong Huang0Qi Li1Shiping Yang2Yunhao Yuan3Zhiqiang Zhang4Baijian Jiang5Jing Lv6Jian Zhong7Jichang Jian8Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524094, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524094, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524094, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524094, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524094, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524094, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524094, ChinaZhanjiang Customs District People’s Republic of China, Zhanjiang 524000, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, College of Fishery, Guangdong Ocean University, Zhanjiang 524094, ChinaHeme oxygenase-1 (HO-1) is a stress-inducible enzyme with antioxidant, anti-inflammatory, and anti-apoptotic effects. In this study, the <i>HO-1</i> gene from <i>Litopenaeus vannamei</i> (<i>Lv-HO-1</i>) was identified. The open reading frame of <i>Lv-HO-1</i> is 747 bp, encoding a peptide of 248 amino acids as well as a conserved HemO structural domain. Lv-HO-1 is 70–90% homological to crustaceans and about 50% homological to arthropods. The transcript levels of <i>Lv-HO-1</i> were highest in the hepatopancreas and lower in other tissues. Knockdown of <i>Lv-HO-1</i> led to structural destruction of the hepatopancreas. After ammonia exposure, <i>Lv-HO-1</i> was significantly induced. Knockdown of <i>Lv-HO-1</i> during ammonia exposure resulted in a significant decrease in antioxidant capacity and cellular autophagy levels compared to the control and increased apoptosis. The transcriptional levels of <i>SOD</i> and <i>GSH-Px</i> were considerably reduced (<i>p</i> < 0.05), as were the transcriptional levels of <i>Atg3</i>, <i>Atg4</i>, <i>Atg5</i>, and <i>Atg10</i>. The results indicated that <i>Lv-HO-1</i> from <i>L. vannamei</i> can be induced by oxidative stress and may have important roles in regulating the host antioxidant system, reducing cell apoptosis.https://www.mdpi.com/2410-3888/7/6/356<i>HO-1</i>RNA interference<i>Litopenaeus vannamei</i>oxidative stressapoptosisautophagy |
| spellingShingle | Yongxiong Huang Qi Li Shiping Yang Yunhao Yuan Zhiqiang Zhang Baijian Jiang Jing Lv Jian Zhong Jichang Jian Identification and Characterization of <i>Heme Oxygenase-1</i> from <i>Litopenaeus vannamei</i> Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress Fishes <i>HO-1</i> RNA interference <i>Litopenaeus vannamei</i> oxidative stress apoptosis autophagy |
| title | Identification and Characterization of <i>Heme Oxygenase-1</i> from <i>Litopenaeus vannamei</i> Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress |
| title_full | Identification and Characterization of <i>Heme Oxygenase-1</i> from <i>Litopenaeus vannamei</i> Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress |
| title_fullStr | Identification and Characterization of <i>Heme Oxygenase-1</i> from <i>Litopenaeus vannamei</i> Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress |
| title_full_unstemmed | Identification and Characterization of <i>Heme Oxygenase-1</i> from <i>Litopenaeus vannamei</i> Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress |
| title_short | Identification and Characterization of <i>Heme Oxygenase-1</i> from <i>Litopenaeus vannamei</i> Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress |
| title_sort | identification and characterization of i heme oxygenase 1 i from i litopenaeus vannamei i involved in antioxidant and anti apoptosis under ammonia stress |
| topic | <i>HO-1</i> RNA interference <i>Litopenaeus vannamei</i> oxidative stress apoptosis autophagy |
| url | https://www.mdpi.com/2410-3888/7/6/356 |
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