Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction
Introduction: Obesity is a potential risk factor for erectile dysfunction (ED). MicroRNAs (miRNAs) regulate the expression of genes involved in various pathophysiologic processes. Aim: To identify the miRNA profile in the corpus cavernosum (CC) of obese rats with ED and elucidate the potential funct...
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| Format: | Article |
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Oxford University Press
2017-12-01
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| Series: | Sexual Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2050116117300570 |
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| author | Yunlong Bai, PhD Liangshuan Zhang, PhD Yanan Jiang, PhD Jiaming Ju, MS Guiyang Li, MS Juan Xu, PhD Xing Jiang, MS Peng Zhang, MS Linchuan Lang, MS Olga Sadkovaya, MPH Peter V. Glybochko, DMSc Wei Zhang, MS Baofeng Yang, PhD |
| author_facet | Yunlong Bai, PhD Liangshuan Zhang, PhD Yanan Jiang, PhD Jiaming Ju, MS Guiyang Li, MS Juan Xu, PhD Xing Jiang, MS Peng Zhang, MS Linchuan Lang, MS Olga Sadkovaya, MPH Peter V. Glybochko, DMSc Wei Zhang, MS Baofeng Yang, PhD |
| author_sort | Yunlong Bai, PhD |
| collection | DOAJ |
| description | Introduction: Obesity is a potential risk factor for erectile dysfunction (ED). MicroRNAs (miRNAs) regulate the expression of genes involved in various pathophysiologic processes.
Aim: To identify the miRNA profile in the corpus cavernosum (CC) of obese rats with ED and elucidate the potential function of miRNA in the pathogenesis of ED.
Methods: Obesity was induced in rats by a high-fat diet. After the erectile function test, experimental animals were divided into two groups: obese rats with ED and obese rats with normal erectile function. The CCs from these rats were collected for miRNA microarray analysis. The results were verified by real-time polymerase chain reaction analysis. Subsequently, the targets of differentially expressed miRNAs were predicted. Bioinformatics analysis was applied to predict the functions of differentially expressed miRNAs in ED. Apomorphine-induced penile erection and intracavernous pressure measurements were used to evaluate the effects of miRNA on the erectile function of rats.
Main Outcome Measures: MiRNA expression in the CC of obese rats with ED and those with normal erectile function was detected by miRNA microarray analysis. Candidate miRNAs were validated by real-time polymerase chain reaction. Bioinformatics analysis was used to predict the functions of miRNAs. Apomorphine-induced penile erection and intracavernous pressure measurements were used to reflect the erectile function of rats.
Results: Sixty-eight miRNAs were differentially expressed in the CC of obese rats with ED (≥1.5-fold change). The real-time polymerase chain reaction results were consistent with the miRNA microarray analysis results. Specifically, miR-328a was significantly upregulated in rats with ED compared with control rats and was chosen for functional evaluation in the pathogenesis of ED. Overexpression of miR-328a noticeably decreased the erectile response to apomorphine and the expression of heme oxygenase-1.
Conclusion: MiRNAs are involved in the pathogenesis of obesity-related ED. MiR-328a might facilitate the induction of ED.
Bai Y, Zhang L, Jiang Y, et al. Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction. Sex Med 2017;5:e261–e271. |
| first_indexed | 2024-03-12T19:50:18Z |
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| id | doaj.art-ad75b69bc36a4d5fa9c7e820663beaff |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-12T19:50:18Z |
| publishDate | 2017-12-01 |
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| spelling | doaj.art-ad75b69bc36a4d5fa9c7e820663beaff2023-08-02T03:16:12ZengOxford University PressSexual Medicine2050-11612017-12-0154e261e27110.1016/j.esxm.2017.06.006Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile DysfunctionYunlong Bai, PhD0Liangshuan Zhang, PhD1Yanan Jiang, PhD2Jiaming Ju, MS3Guiyang Li, MS4Juan Xu, PhD5Xing Jiang, MS6Peng Zhang, MS7Linchuan Lang, MS8Olga Sadkovaya, MPH9Peter V. Glybochko, DMSc10Wei Zhang, MS11Baofeng Yang, PhD12Department of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People’s Republic of ChinaDepartment of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People’s Republic of ChinaDepartment of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People’s Republic of ChinaNorth China Translational Medicine Research and Cooperation Center, Harbin Medical University, Harbin, People’s Republic of ChinaDepartment of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People’s Republic of ChinaCollege of Bioinformatics Science and Technology, Harbin Medical University, Harbin, People’s Republic of ChinaDepartment of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People’s Republic of ChinaDepartment of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People’s Republic of ChinaDepartment of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People’s Republic of ChinaSechenov First Moscow State Medical University, Moscow, RussiaSechenov First Moscow State Medical University, Moscow, RussiaNorth China Translational Medicine Research and Cooperation Center, Harbin Medical University, Harbin, People’s Republic of ChinaDepartment of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People’s Republic of ChinaIntroduction: Obesity is a potential risk factor for erectile dysfunction (ED). MicroRNAs (miRNAs) regulate the expression of genes involved in various pathophysiologic processes. Aim: To identify the miRNA profile in the corpus cavernosum (CC) of obese rats with ED and elucidate the potential function of miRNA in the pathogenesis of ED. Methods: Obesity was induced in rats by a high-fat diet. After the erectile function test, experimental animals were divided into two groups: obese rats with ED and obese rats with normal erectile function. The CCs from these rats were collected for miRNA microarray analysis. The results were verified by real-time polymerase chain reaction analysis. Subsequently, the targets of differentially expressed miRNAs were predicted. Bioinformatics analysis was applied to predict the functions of differentially expressed miRNAs in ED. Apomorphine-induced penile erection and intracavernous pressure measurements were used to evaluate the effects of miRNA on the erectile function of rats. Main Outcome Measures: MiRNA expression in the CC of obese rats with ED and those with normal erectile function was detected by miRNA microarray analysis. Candidate miRNAs were validated by real-time polymerase chain reaction. Bioinformatics analysis was used to predict the functions of miRNAs. Apomorphine-induced penile erection and intracavernous pressure measurements were used to reflect the erectile function of rats. Results: Sixty-eight miRNAs were differentially expressed in the CC of obese rats with ED (≥1.5-fold change). The real-time polymerase chain reaction results were consistent with the miRNA microarray analysis results. Specifically, miR-328a was significantly upregulated in rats with ED compared with control rats and was chosen for functional evaluation in the pathogenesis of ED. Overexpression of miR-328a noticeably decreased the erectile response to apomorphine and the expression of heme oxygenase-1. Conclusion: MiRNAs are involved in the pathogenesis of obesity-related ED. MiR-328a might facilitate the induction of ED. Bai Y, Zhang L, Jiang Y, et al. Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction. Sex Med 2017;5:e261–e271.http://www.sciencedirect.com/science/article/pii/S2050116117300570ObesityErectile DysfunctionMicroRNA Microarray AnalysisMiR-328a |
| spellingShingle | Yunlong Bai, PhD Liangshuan Zhang, PhD Yanan Jiang, PhD Jiaming Ju, MS Guiyang Li, MS Juan Xu, PhD Xing Jiang, MS Peng Zhang, MS Linchuan Lang, MS Olga Sadkovaya, MPH Peter V. Glybochko, DMSc Wei Zhang, MS Baofeng Yang, PhD Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction Sexual Medicine Obesity Erectile Dysfunction MicroRNA Microarray Analysis MiR-328a |
| title | Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction |
| title_full | Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction |
| title_fullStr | Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction |
| title_full_unstemmed | Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction |
| title_short | Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction |
| title_sort | identification and functional verification of micrornas in the obese rat with erectile dysfunction |
| topic | Obesity Erectile Dysfunction MicroRNA Microarray Analysis MiR-328a |
| url | http://www.sciencedirect.com/science/article/pii/S2050116117300570 |
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