The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini)
Three cryptic species of the rodent Akodon (A. cursor, A. montensis and Akodon sp.) were analyzed. The two former species are sympatric in the Brazilian states of São Paulo, Rio de Janeiro and Minas Gerais, where hybrids have already been found. The third species, Akodon sp., occurs in an isolated a...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2007-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400031 |
| _version_ | 1819266687409586176 |
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| author | Valéria Fagundes Cristina Dornelas de Andrade Nogueira |
| author_facet | Valéria Fagundes Cristina Dornelas de Andrade Nogueira |
| author_sort | Valéria Fagundes |
| collection | DOAJ |
| description | Three cryptic species of the rodent Akodon (A. cursor, A. montensis and Akodon sp.) were analyzed. The two former species are sympatric in the Brazilian states of São Paulo, Rio de Janeiro and Minas Gerais, where hybrids have already been found. The third species, Akodon sp., occurs in an isolated area in Central Brazil. The identification of these species is difficult by the need of living animals. At present, karyotyping is the only method used in the identification of specimens. We used PCR-RFLP of the mitochondrial cytochrome gene to test the distinctiveness of the three species, which was confirmed by the absence of shared species-specific haplotypes. We also detected a geographical pattern of haplotypes distribution with highly polymorphic populations of A. cursor from Espírito Santo and of A. montensis from Rio Grande do Sul. |
| first_indexed | 2024-12-23T21:05:14Z |
| format | Article |
| id | doaj.art-ad772264cd944157bf190a44f2b7c94d |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-23T21:05:14Z |
| publishDate | 2007-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-ad772264cd944157bf190a44f2b7c94d2022-12-21T17:31:15ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852007-01-0130369870110.1590/S1415-47572007000400031The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini)Valéria FagundesCristina Dornelas de Andrade NogueiraThree cryptic species of the rodent Akodon (A. cursor, A. montensis and Akodon sp.) were analyzed. The two former species are sympatric in the Brazilian states of São Paulo, Rio de Janeiro and Minas Gerais, where hybrids have already been found. The third species, Akodon sp., occurs in an isolated area in Central Brazil. The identification of these species is difficult by the need of living animals. At present, karyotyping is the only method used in the identification of specimens. We used PCR-RFLP of the mitochondrial cytochrome gene to test the distinctiveness of the three species, which was confirmed by the absence of shared species-specific haplotypes. We also detected a geographical pattern of haplotypes distribution with highly polymorphic populations of A. cursor from Espírito Santo and of A. montensis from Rio Grande do Sul.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400031Akodon cursorAkodon montensisPCR-RFLPSigmodontinae |
| spellingShingle | Valéria Fagundes Cristina Dornelas de Andrade Nogueira The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini) Genetics and Molecular Biology Akodon cursor Akodon montensis PCR-RFLP Sigmodontinae |
| title | The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini) |
| title_full | The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini) |
| title_fullStr | The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini) |
| title_full_unstemmed | The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini) |
| title_short | The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini) |
| title_sort | use of pcr rflp as an identification tool for three closely related species of rodents of the genus akodon sigmodontinae akodontini |
| topic | Akodon cursor Akodon montensis PCR-RFLP Sigmodontinae |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400031 |
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