Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene, <i>Me-col-1</i>, in Root-Knot Nematode <i>Meloidogyne enterolobii</i>
<i>Meloidogyne enterolobii</i>, a highly pathogenic root-knot nematode species, causes serious damage to agricultural production worldwide. Collagen is an important part of the nematode epidermis, which is crucial for nematode shape maintenance, motility, and reproduction. In this study,...
| المؤلفون الرئيسيون: | , , , , , |
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| التنسيق: | مقال |
| اللغة: | English |
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MDPI AG
2022-12-01
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| سلاسل: | Life |
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| الوصول للمادة أونلاين: | https://www.mdpi.com/2075-1729/12/12/2103 |
| _version_ | 1827638055469580288 |
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| author | Ji Pei Tuizi Feng Haibo Long Yuan Chen Yueling Pei Yanfang Sun |
| author_facet | Ji Pei Tuizi Feng Haibo Long Yuan Chen Yueling Pei Yanfang Sun |
| author_sort | Ji Pei |
| collection | DOAJ |
| description | <i>Meloidogyne enterolobii</i>, a highly pathogenic root-knot nematode species, causes serious damage to agricultural production worldwide. Collagen is an important part of the nematode epidermis, which is crucial for nematode shape maintenance, motility, and reproduction. In this study, we report that a novel collagen gene, <i>Me-col-1,</i> from the highly pathogenic root-knot nematode species <i>Meloidogyne enterolobi</i> was required for the egg formation of this pathogen. <i>Me-col-1</i> encodes a protein with the size of 35 kDa, which is closely related to collagen found in other nematodes. Real-time PCR assays showed that the expression of <i>Me-col-1</i> was highest in eggs and lowest in pre-parasitic second-stage juveniles (preJ2). Interestingly, knockdown of <i>Me-col-1</i> did not compromise the survival rate of preJ2 but significantly reduced the egg production and consequentially caused 35.79% lower multiplication rate (Pf/Pi) compared with control. Our study provides valuable information for better understanding the function of collagen genes in the nematode life cycle, which can be used in the development of effective approaches for nematode control. |
| first_indexed | 2024-03-09T16:11:15Z |
| format | Article |
| id | doaj.art-ad799b25592d40428c24ca85638e15ab |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T16:11:15Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-ad799b25592d40428c24ca85638e15ab2023-11-24T16:13:35ZengMDPI AGLife2075-17292022-12-011212210310.3390/life12122103Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene, <i>Me-col-1</i>, in Root-Knot Nematode <i>Meloidogyne enterolobii</i>Ji Pei0Tuizi Feng1Haibo Long2Yuan Chen3Yueling Pei4Yanfang Sun5College of Plant Protection/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, Hainan University, Haikou 570228, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs, Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China<i>Meloidogyne enterolobii</i>, a highly pathogenic root-knot nematode species, causes serious damage to agricultural production worldwide. Collagen is an important part of the nematode epidermis, which is crucial for nematode shape maintenance, motility, and reproduction. In this study, we report that a novel collagen gene, <i>Me-col-1,</i> from the highly pathogenic root-knot nematode species <i>Meloidogyne enterolobi</i> was required for the egg formation of this pathogen. <i>Me-col-1</i> encodes a protein with the size of 35 kDa, which is closely related to collagen found in other nematodes. Real-time PCR assays showed that the expression of <i>Me-col-1</i> was highest in eggs and lowest in pre-parasitic second-stage juveniles (preJ2). Interestingly, knockdown of <i>Me-col-1</i> did not compromise the survival rate of preJ2 but significantly reduced the egg production and consequentially caused 35.79% lower multiplication rate (Pf/Pi) compared with control. Our study provides valuable information for better understanding the function of collagen genes in the nematode life cycle, which can be used in the development of effective approaches for nematode control.https://www.mdpi.com/2075-1729/12/12/2103<i>Meloidogyne enterolobii</i>root-knot nematodecollagensVIGSRNAi |
| spellingShingle | Ji Pei Tuizi Feng Haibo Long Yuan Chen Yueling Pei Yanfang Sun Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene, <i>Me-col-1</i>, in Root-Knot Nematode <i>Meloidogyne enterolobii</i> Life <i>Meloidogyne enterolobii</i> root-knot nematode collagens VIGS RNAi |
| title | Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene, <i>Me-col-1</i>, in Root-Knot Nematode <i>Meloidogyne enterolobii</i> |
| title_full | Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene, <i>Me-col-1</i>, in Root-Knot Nematode <i>Meloidogyne enterolobii</i> |
| title_fullStr | Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene, <i>Me-col-1</i>, in Root-Knot Nematode <i>Meloidogyne enterolobii</i> |
| title_full_unstemmed | Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene, <i>Me-col-1</i>, in Root-Knot Nematode <i>Meloidogyne enterolobii</i> |
| title_short | Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene, <i>Me-col-1</i>, in Root-Knot Nematode <i>Meloidogyne enterolobii</i> |
| title_sort | molecular characterization and virus induced gene silencing of a collagen gene i me col 1 i in root knot nematode i meloidogyne enterolobii i |
| topic | <i>Meloidogyne enterolobii</i> root-knot nematode collagens VIGS RNAi |
| url | https://www.mdpi.com/2075-1729/12/12/2103 |
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