Response of the Human Milk Microbiota to a Maternal Prebiotic Intervention Is Individual and Influenced by Maternal Age
Maternal bacteria are shared with infants via breastfeeding. Prebiotics modulate the gut microbiota, promoting health benefits. We investigated whether the maternal diet supplementation with a prebiotic (fructooligosaccharides, FOS) could influence the milk microbiota. Twenty-eight lactating women r...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/12/4/1081 |
| _version_ | 1797570717528621056 |
|---|---|
| author | Marina Padilha Asker Brejnrod Niels Banhos Danneskiold-Samsøe Christian Hoffmann Julia de Melo Iaucci Vanessa Pereira Cabral Douglas Xavier-Santos Carla Romano Taddei Karsten Kristiansen Susana Marta Isay Saad |
| author_facet | Marina Padilha Asker Brejnrod Niels Banhos Danneskiold-Samsøe Christian Hoffmann Julia de Melo Iaucci Vanessa Pereira Cabral Douglas Xavier-Santos Carla Romano Taddei Karsten Kristiansen Susana Marta Isay Saad |
| author_sort | Marina Padilha |
| collection | DOAJ |
| description | Maternal bacteria are shared with infants via breastfeeding. Prebiotics modulate the gut microbiota, promoting health benefits. We investigated whether the maternal diet supplementation with a prebiotic (fructooligosaccharides, FOS) could influence the milk microbiota. Twenty-eight lactating women received 4.5 g of fructooligosaccharides + 2 g of maltodextrin (FOS group) and twenty-five received 2 g of maltodextrin (placebo group) for 20 days. Breast-milk samples were taken before and after the intervention. The DNA from samples was used for 16S rRNA sequencing. No statistical differences between the groups were found for the bacterial genera after the intervention. However, the distances of the trajectories covered by paired samples from the beginning to the end of the supplementation were higher for the FOS group (<i>p</i> = 0.0007) indicating greater changes in milk microbiota compared to the control group. Linear regression models suggested that the maternal age influenced the response for FOS supplementation (<i>p</i> = 0.02). Interestingly, the pattern of changes to genus abundance upon supplementation was not shared between mothers. We demonstrated that manipulating the human milk microbiota through prebiotics is possible, and the maternal age can affect this response. |
| first_indexed | 2024-03-10T20:29:16Z |
| format | Article |
| id | doaj.art-ad7a178bbab445488550f39aa7bd04a5 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T20:29:16Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-ad7a178bbab445488550f39aa7bd04a52023-11-19T21:31:34ZengMDPI AGNutrients2072-66432020-04-01124108110.3390/nu12041081Response of the Human Milk Microbiota to a Maternal Prebiotic Intervention Is Individual and Influenced by Maternal AgeMarina Padilha0Asker Brejnrod1Niels Banhos Danneskiold-Samsøe2Christian Hoffmann3Julia de Melo Iaucci4Vanessa Pereira Cabral5Douglas Xavier-Santos6Carla Romano Taddei7Karsten Kristiansen8Susana Marta Isay Saad9School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, BrazilLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, DK-2100 Copenhagen, DenmarkLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, DK-2100 Copenhagen, DenmarkSchool of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, BrazilSchool of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, BrazilSchool of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, BrazilSchool of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, BrazilSchool of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, BrazilLaboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, DK-2100 Copenhagen, DenmarkSchool of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, BrazilMaternal bacteria are shared with infants via breastfeeding. Prebiotics modulate the gut microbiota, promoting health benefits. We investigated whether the maternal diet supplementation with a prebiotic (fructooligosaccharides, FOS) could influence the milk microbiota. Twenty-eight lactating women received 4.5 g of fructooligosaccharides + 2 g of maltodextrin (FOS group) and twenty-five received 2 g of maltodextrin (placebo group) for 20 days. Breast-milk samples were taken before and after the intervention. The DNA from samples was used for 16S rRNA sequencing. No statistical differences between the groups were found for the bacterial genera after the intervention. However, the distances of the trajectories covered by paired samples from the beginning to the end of the supplementation were higher for the FOS group (<i>p</i> = 0.0007) indicating greater changes in milk microbiota compared to the control group. Linear regression models suggested that the maternal age influenced the response for FOS supplementation (<i>p</i> = 0.02). Interestingly, the pattern of changes to genus abundance upon supplementation was not shared between mothers. We demonstrated that manipulating the human milk microbiota through prebiotics is possible, and the maternal age can affect this response.https://www.mdpi.com/2072-6643/12/4/1081fructooligosaccharidebreast milkbreastfeedinginfantclinical interventionmicroorganisms |
| spellingShingle | Marina Padilha Asker Brejnrod Niels Banhos Danneskiold-Samsøe Christian Hoffmann Julia de Melo Iaucci Vanessa Pereira Cabral Douglas Xavier-Santos Carla Romano Taddei Karsten Kristiansen Susana Marta Isay Saad Response of the Human Milk Microbiota to a Maternal Prebiotic Intervention Is Individual and Influenced by Maternal Age Nutrients fructooligosaccharide breast milk breastfeeding infant clinical intervention microorganisms |
| title | Response of the Human Milk Microbiota to a Maternal Prebiotic Intervention Is Individual and Influenced by Maternal Age |
| title_full | Response of the Human Milk Microbiota to a Maternal Prebiotic Intervention Is Individual and Influenced by Maternal Age |
| title_fullStr | Response of the Human Milk Microbiota to a Maternal Prebiotic Intervention Is Individual and Influenced by Maternal Age |
| title_full_unstemmed | Response of the Human Milk Microbiota to a Maternal Prebiotic Intervention Is Individual and Influenced by Maternal Age |
| title_short | Response of the Human Milk Microbiota to a Maternal Prebiotic Intervention Is Individual and Influenced by Maternal Age |
| title_sort | response of the human milk microbiota to a maternal prebiotic intervention is individual and influenced by maternal age |
| topic | fructooligosaccharide breast milk breastfeeding infant clinical intervention microorganisms |
| url | https://www.mdpi.com/2072-6643/12/4/1081 |
| work_keys_str_mv | AT marinapadilha responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage AT askerbrejnrod responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage AT nielsbanhosdanneskioldsamsøe responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage AT christianhoffmann responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage AT juliademeloiaucci responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage AT vanessapereiracabral responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage AT douglasxaviersantos responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage AT carlaromanotaddei responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage AT karstenkristiansen responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage AT susanamartaisaysaad responseofthehumanmilkmicrobiotatoamaternalprebioticinterventionisindividualandinfluencedbymaternalage |