The influence of size and surface chemistry on the bioavailability, tissue distribution and toxicity of gold nanoparticles in zebrafish (Danio rerio)
Gold nanoparticles (AuNPs) are widely used in biomedicine and their specific properties including, size, geometrics, and surface coating, will affect their fate and behaviour in biological systems. These properties are well studied for their intended biological targets, but there is a lack of unders...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323005237 |
| _version_ | 1797806391939825664 |
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| author | Dylan L. Windell Sulayman Mourabit Julian Moger Stewart F. Owen Matthew J. Winter Charles R. Tyler |
| author_facet | Dylan L. Windell Sulayman Mourabit Julian Moger Stewart F. Owen Matthew J. Winter Charles R. Tyler |
| author_sort | Dylan L. Windell |
| collection | DOAJ |
| description | Gold nanoparticles (AuNPs) are widely used in biomedicine and their specific properties including, size, geometrics, and surface coating, will affect their fate and behaviour in biological systems. These properties are well studied for their intended biological targets, but there is a lack of understanding on the mechanisms by which AuNPs interact in non-target organisms when they enter the environment. We investigated the effects of size and surface chemistry of AuNPs on their bioavailability, tissue distribution and potential toxicity using zebrafish (Danio rerio) as an experimental model. Larval zebrafish were exposed to fluorescently tagged AuNPs of different sizes (10–100 nm) and surface modifications (TNFα, NHS/PAMAM and PEG), and uptake, tissue distribution and depuration rates were measured using selective-plane illumination microscopy (SPIM). The gut and pronephric tubules were found to contain detectable levels of AuNPs, and the concentration-dependent accumulation was related to the particle size. Surface addition of PEG and TNFα appeared to enhance particle accumulation in the pronephric tubules compared to uncoated particles. Depuration studies showed a gradual removal of particles from the gut and pronephric tubules, although fluorescence indicating the presence of the AuNPs remained in the pronephros 96 h after exposure. Toxicity assessment using two transgenic zebrafish reporter lines, however, revealed no AuNP-related renal injury or cellular oxidative stress. Collectively, our data show that AuNPs used in medical applications across the size range 40–80 nm, are bioavailable to larval zebrafish and some may persist in renal tissue, although their presence did not result in measurable toxicity with respect to pronephric organ function or cellular oxidative stress for short term exposures. |
| first_indexed | 2024-03-13T06:06:36Z |
| format | Article |
| id | doaj.art-ad7bde81009e42c78aaa07105d0de375 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-13T06:06:36Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-ad7bde81009e42c78aaa07105d0de3752023-06-12T04:08:55ZengElsevierEcotoxicology and Environmental Safety0147-65132023-07-01260115019The influence of size and surface chemistry on the bioavailability, tissue distribution and toxicity of gold nanoparticles in zebrafish (Danio rerio)Dylan L. Windell0Sulayman Mourabit1Julian Moger2Stewart F. Owen3Matthew J. Winter4Charles R. Tyler5Biosciences, Faculty of Health and Life Sciences, Exeter, Devon EX4 4QD, United KingdomBiosciences, Faculty of Health and Life Sciences, Exeter, Devon EX4 4QD, United KingdomPhysics and Medical Imaging, College of Engineering, Mathematics and Physical Sciences, University of Exeter, Exeter, Devon EX4 4QL, United KingdomAstraZeneca, Global Compliance, Alderley Park, Macclesfield, Cheshire SK10 4TF, United KingdomBiosciences, Faculty of Health and Life Sciences, Exeter, Devon EX4 4QD, United KingdomBiosciences, Faculty of Health and Life Sciences, Exeter, Devon EX4 4QD, United Kingdom; Correspondence to: Biosciences, Faculty of Health and Life Sciences, University of Exeter, Geoffrey Pope Building, Exeter, Devon EX4 4QD, United Kingdom.Gold nanoparticles (AuNPs) are widely used in biomedicine and their specific properties including, size, geometrics, and surface coating, will affect their fate and behaviour in biological systems. These properties are well studied for their intended biological targets, but there is a lack of understanding on the mechanisms by which AuNPs interact in non-target organisms when they enter the environment. We investigated the effects of size and surface chemistry of AuNPs on their bioavailability, tissue distribution and potential toxicity using zebrafish (Danio rerio) as an experimental model. Larval zebrafish were exposed to fluorescently tagged AuNPs of different sizes (10–100 nm) and surface modifications (TNFα, NHS/PAMAM and PEG), and uptake, tissue distribution and depuration rates were measured using selective-plane illumination microscopy (SPIM). The gut and pronephric tubules were found to contain detectable levels of AuNPs, and the concentration-dependent accumulation was related to the particle size. Surface addition of PEG and TNFα appeared to enhance particle accumulation in the pronephric tubules compared to uncoated particles. Depuration studies showed a gradual removal of particles from the gut and pronephric tubules, although fluorescence indicating the presence of the AuNPs remained in the pronephros 96 h after exposure. Toxicity assessment using two transgenic zebrafish reporter lines, however, revealed no AuNP-related renal injury or cellular oxidative stress. Collectively, our data show that AuNPs used in medical applications across the size range 40–80 nm, are bioavailable to larval zebrafish and some may persist in renal tissue, although their presence did not result in measurable toxicity with respect to pronephric organ function or cellular oxidative stress for short term exposures.http://www.sciencedirect.com/science/article/pii/S0147651323005237SPIMLight sheet microscopyGold nanoparticlesNanotoxicologyZebrafish |
| spellingShingle | Dylan L. Windell Sulayman Mourabit Julian Moger Stewart F. Owen Matthew J. Winter Charles R. Tyler The influence of size and surface chemistry on the bioavailability, tissue distribution and toxicity of gold nanoparticles in zebrafish (Danio rerio) Ecotoxicology and Environmental Safety SPIM Light sheet microscopy Gold nanoparticles Nanotoxicology Zebrafish |
| title | The influence of size and surface chemistry on the bioavailability, tissue distribution and toxicity of gold nanoparticles in zebrafish (Danio rerio) |
| title_full | The influence of size and surface chemistry on the bioavailability, tissue distribution and toxicity of gold nanoparticles in zebrafish (Danio rerio) |
| title_fullStr | The influence of size and surface chemistry on the bioavailability, tissue distribution and toxicity of gold nanoparticles in zebrafish (Danio rerio) |
| title_full_unstemmed | The influence of size and surface chemistry on the bioavailability, tissue distribution and toxicity of gold nanoparticles in zebrafish (Danio rerio) |
| title_short | The influence of size and surface chemistry on the bioavailability, tissue distribution and toxicity of gold nanoparticles in zebrafish (Danio rerio) |
| title_sort | influence of size and surface chemistry on the bioavailability tissue distribution and toxicity of gold nanoparticles in zebrafish danio rerio |
| topic | SPIM Light sheet microscopy Gold nanoparticles Nanotoxicology Zebrafish |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323005237 |
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