A pan-cancer analysis of potassium channel tetramerization domain containing 12 in human cancer
Abstract Abnormal expression of the potassium channel tetramerization domain containing 12 (KCTD12) is closely related to the occurrence and development of various tumors, but a pan-cancer analysis of KCTD12 has not yet been conducted. We explored the association between KCTD12 and more than 30 huma...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41091-8 |
| _version_ | 1797559850921623552 |
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| author | Pan Liu Zhilan Liu Qiankun Luo Qiang Fu Xu Zhang Pengfei Yu Shuai Zhou Yingying Wang Jiali Zhang Song Chen Hongwei Zhang Qinghai Zhu Tao Qin |
| author_facet | Pan Liu Zhilan Liu Qiankun Luo Qiang Fu Xu Zhang Pengfei Yu Shuai Zhou Yingying Wang Jiali Zhang Song Chen Hongwei Zhang Qinghai Zhu Tao Qin |
| author_sort | Pan Liu |
| collection | DOAJ |
| description | Abstract Abnormal expression of the potassium channel tetramerization domain containing 12 (KCTD12) is closely related to the occurrence and development of various tumors, but a pan-cancer analysis of KCTD12 has not yet been conducted. We explored the association between KCTD12 and more than 30 human malignancies using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. First, the mRNA and protein levels of KCTD12 were examined and their correlations with tumor stage and survival were explored. Second, we analyzed the infiltration of CD8+ and CD4+ T cells and cancer-associated fibroblasts in tumors and explored the correlation between KCTD12 expression and tumor cell stemness, genomic heterogeneity, and diagnostic specificity. Finally, we explored the molecular mechanisms associated with KCTD12 using KEGG/GO analysis. The results showed that KCTD12 mRNA and protein expression levels decreased in most tumors was significantly associated with the prognosis of tumor patients, and the phosphorylation level of KCTD12 decreased in several tumors, such as S200 and T196, pancreatic adenocarcinoma (PAAD), lung adenocarcinoma (LUAD), and breast invasive cancer (BRCA). The expression of KCTD12 was positively correlated with the degree of cancer-associated fibroblasts infiltration in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), head and neck squamous cell carcinoma (HNSC), PAAD, and stomach adenocarcinoma (STAD). The relationship between KCTD12 expression and CD8+ and CD4+ T cell infiltration was also clarified. KCTD12 showed high diagnostic sensitivity for various types of tumors and may be involved in tumor cell biology by affecting tumor cell stemness, tumor burden, and other characteristics. Finally, we analyzed the molecular functions of KCTD12 and possible KEGG/GO signaling pathways. In this study, we developed a biological marker for diagnosis, prognosis, and immune infiltration of the pan-cancers. |
| first_indexed | 2024-03-10T17:52:05Z |
| format | Article |
| id | doaj.art-ad7f707a1e4a4818a6dbe8fe7966cd08 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:52:05Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ad7f707a1e4a4818a6dbe8fe7966cd082023-11-20T09:20:37ZengNature PortfolioScientific Reports2045-23222023-08-0113111510.1038/s41598-023-41091-8A pan-cancer analysis of potassium channel tetramerization domain containing 12 in human cancerPan Liu0Zhilan Liu1Qiankun Luo2Qiang Fu3Xu Zhang4Pengfei Yu5Shuai Zhou6Yingying Wang7Jiali Zhang8Song Chen9Hongwei Zhang10Qinghai Zhu11Tao Qin12Department of Hepato-Biliary-Pancreatic Surgery, Zhengzhou University People’s Hospital (Henan Provincial People’s Hospital)Henan University of Chinese MedicineDepartment of Hepato-Biliary-Pancreatic Surgery, Zhengzhou University People’s Hospital (Henan Provincial People’s Hospital)Department of Hepato-Biliary-Pancreatic Surgery, Zhengzhou University People’s Hospital (Henan Provincial People’s Hospital)Department of Hepato-Biliary-Pancreatic Surgery, Zhengzhou University People’s Hospital (Henan Provincial People’s Hospital)Department of Hepato-Biliary-Pancreatic Surgery, Zhengzhou University People’s Hospital (Henan Provincial People’s Hospital)Translational Research Institute, Zhengzhou University People’s HospitalAcademy of Medical Sciences, Zhengzhou UniversityAcademy of Medical Sciences, Zhengzhou UniversityTranslational Research Institute, Zhengzhou University People’s HospitalDepartment of Hepato-Biliary-Pancreatic Surgery, Zhengzhou University People’s Hospital (Henan Provincial People’s Hospital)Zhumadian Central HospitalDepartment of Hepato-Biliary-Pancreatic Surgery, Zhengzhou University People’s Hospital (Henan Provincial People’s Hospital)Abstract Abnormal expression of the potassium channel tetramerization domain containing 12 (KCTD12) is closely related to the occurrence and development of various tumors, but a pan-cancer analysis of KCTD12 has not yet been conducted. We explored the association between KCTD12 and more than 30 human malignancies using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. First, the mRNA and protein levels of KCTD12 were examined and their correlations with tumor stage and survival were explored. Second, we analyzed the infiltration of CD8+ and CD4+ T cells and cancer-associated fibroblasts in tumors and explored the correlation between KCTD12 expression and tumor cell stemness, genomic heterogeneity, and diagnostic specificity. Finally, we explored the molecular mechanisms associated with KCTD12 using KEGG/GO analysis. The results showed that KCTD12 mRNA and protein expression levels decreased in most tumors was significantly associated with the prognosis of tumor patients, and the phosphorylation level of KCTD12 decreased in several tumors, such as S200 and T196, pancreatic adenocarcinoma (PAAD), lung adenocarcinoma (LUAD), and breast invasive cancer (BRCA). The expression of KCTD12 was positively correlated with the degree of cancer-associated fibroblasts infiltration in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), head and neck squamous cell carcinoma (HNSC), PAAD, and stomach adenocarcinoma (STAD). The relationship between KCTD12 expression and CD8+ and CD4+ T cell infiltration was also clarified. KCTD12 showed high diagnostic sensitivity for various types of tumors and may be involved in tumor cell biology by affecting tumor cell stemness, tumor burden, and other characteristics. Finally, we analyzed the molecular functions of KCTD12 and possible KEGG/GO signaling pathways. In this study, we developed a biological marker for diagnosis, prognosis, and immune infiltration of the pan-cancers.https://doi.org/10.1038/s41598-023-41091-8 |
| spellingShingle | Pan Liu Zhilan Liu Qiankun Luo Qiang Fu Xu Zhang Pengfei Yu Shuai Zhou Yingying Wang Jiali Zhang Song Chen Hongwei Zhang Qinghai Zhu Tao Qin A pan-cancer analysis of potassium channel tetramerization domain containing 12 in human cancer Scientific Reports |
| title | A pan-cancer analysis of potassium channel tetramerization domain containing 12 in human cancer |
| title_full | A pan-cancer analysis of potassium channel tetramerization domain containing 12 in human cancer |
| title_fullStr | A pan-cancer analysis of potassium channel tetramerization domain containing 12 in human cancer |
| title_full_unstemmed | A pan-cancer analysis of potassium channel tetramerization domain containing 12 in human cancer |
| title_short | A pan-cancer analysis of potassium channel tetramerization domain containing 12 in human cancer |
| title_sort | pan cancer analysis of potassium channel tetramerization domain containing 12 in human cancer |
| url | https://doi.org/10.1038/s41598-023-41091-8 |
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