Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing
Abstract This study investigated the therapeutic effects of dry-preserved multi-layered fibroblast cell sheets (dry sheets) on cutaneous ulcers. Dry sheets were prepared by air-drying multi-layered fibroblast cell sheets (living sheets) to cease their life activities. Before in vivo application, we...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-16345-6 |
| _version_ | 1818509905519181824 |
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| author | Yutaro Matsuno Masashi Yanagihara Koji Ueno Toshiro Saito Hiroshi Kurazumi Ryo Suzuki Shunsaku Katsura Atsunori Oga Kimikazu Hamano |
| author_facet | Yutaro Matsuno Masashi Yanagihara Koji Ueno Toshiro Saito Hiroshi Kurazumi Ryo Suzuki Shunsaku Katsura Atsunori Oga Kimikazu Hamano |
| author_sort | Yutaro Matsuno |
| collection | DOAJ |
| description | Abstract This study investigated the therapeutic effects of dry-preserved multi-layered fibroblast cell sheets (dry sheets) on cutaneous ulcers. Dry sheets were prepared by air-drying multi-layered fibroblast cell sheets (living sheets) to cease their life activities. Before in vivo application, we tested the release of growth factors into the medium to examine the mechanisms of dry sheets in wound healing. Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) were released from both dry and living sheets, while high levels of fibroblast growth factor-2 (FGF-2) and high mobility group box 1 (HMGB1) protein were only from dry sheets. An in vitro fibroblast proliferation assay revealed that the dry sheet eluate significantly enhanced cell proliferation and VEGF and HGF production compared with living sheet eluate. FGF-2-neutralizing antibodies significantly blocked this proliferative response. In wounds created on diabetic mice, the dry sheet-treatment groups using autologous or allogeneic cells showed significantly accelerated wound closure compared with that in the no-treatment group. The storage stability of the dry sheet was better at refrigeration temperature than at room temperature and remained stable for at least 4 weeks. Our data indicated that allogeneic dry sheets represent a promising new tool for regenerative medicine that promotes wound healing. |
| first_indexed | 2024-12-10T22:51:53Z |
| format | Article |
| id | doaj.art-ad7fc8293cb448bc878cffcef8e76a2e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T22:51:53Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ad7fc8293cb448bc878cffcef8e76a2e2022-12-22T01:30:25ZengNature PortfolioScientific Reports2045-23222022-07-0112111510.1038/s41598-022-16345-6Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healingYutaro Matsuno0Masashi Yanagihara1Koji Ueno2Toshiro Saito3Hiroshi Kurazumi4Ryo Suzuki5Shunsaku Katsura6Atsunori Oga7Kimikazu Hamano8Department of Surgery and Clinical Science, Yamaguchi University Graduate School of MedicineDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of MedicineDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of MedicineDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of MedicineDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of MedicineDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of MedicineDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of MedicineDepartment of Molecular Pathology, Yamaguchi University Graduate School of MedicineDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of MedicineAbstract This study investigated the therapeutic effects of dry-preserved multi-layered fibroblast cell sheets (dry sheets) on cutaneous ulcers. Dry sheets were prepared by air-drying multi-layered fibroblast cell sheets (living sheets) to cease their life activities. Before in vivo application, we tested the release of growth factors into the medium to examine the mechanisms of dry sheets in wound healing. Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) were released from both dry and living sheets, while high levels of fibroblast growth factor-2 (FGF-2) and high mobility group box 1 (HMGB1) protein were only from dry sheets. An in vitro fibroblast proliferation assay revealed that the dry sheet eluate significantly enhanced cell proliferation and VEGF and HGF production compared with living sheet eluate. FGF-2-neutralizing antibodies significantly blocked this proliferative response. In wounds created on diabetic mice, the dry sheet-treatment groups using autologous or allogeneic cells showed significantly accelerated wound closure compared with that in the no-treatment group. The storage stability of the dry sheet was better at refrigeration temperature than at room temperature and remained stable for at least 4 weeks. Our data indicated that allogeneic dry sheets represent a promising new tool for regenerative medicine that promotes wound healing.https://doi.org/10.1038/s41598-022-16345-6 |
| spellingShingle | Yutaro Matsuno Masashi Yanagihara Koji Ueno Toshiro Saito Hiroshi Kurazumi Ryo Suzuki Shunsaku Katsura Atsunori Oga Kimikazu Hamano Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing Scientific Reports |
| title | Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing |
| title_full | Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing |
| title_fullStr | Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing |
| title_full_unstemmed | Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing |
| title_short | Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing |
| title_sort | dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing |
| url | https://doi.org/10.1038/s41598-022-16345-6 |
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