Fe<sub>3</sub>O<sub>4</sub> Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in <span style="font-variant: small-caps">D</span>-Galactose-Induced Aged Mice
Aging is a process associated with blood–brain barrier (BBB) damage and the reduction in neurogenesis, and is the greatest known risk factor for neurodegenerative disorders. However, the effects of Fe<sub>3</sub>O<sub>4</sub> nanozymes on neurogenesis have rarely been studied...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6463 |
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| author | Zihao Xia Manman Gao Peng Sheng Mengmeng Shen Lin Zhao Lizeng Gao Bingchun Yan |
| author_facet | Zihao Xia Manman Gao Peng Sheng Mengmeng Shen Lin Zhao Lizeng Gao Bingchun Yan |
| author_sort | Zihao Xia |
| collection | DOAJ |
| description | Aging is a process associated with blood–brain barrier (BBB) damage and the reduction in neurogenesis, and is the greatest known risk factor for neurodegenerative disorders. However, the effects of Fe<sub>3</sub>O<sub>4</sub> nanozymes on neurogenesis have rarely been studied. This study examined the effects of Fe<sub>3</sub>O<sub>4</sub> nanozymes on neuronal differentiation in the dentate gyrus (DG) and BBB integrity of D-galactose-induced aged mice. Long-term treatment with Fe<sub>3</sub>O<sub>4</sub> nanozymes (10 μg/mL diluted in ddH<sub>2</sub>O daily) markedly increased the doublecortin (DCX) immunoreactivity and decreased BBB injury induced by <span style="font-variant: small-caps;">D</span>-galactose treatment. In addition, the decreases in the levels of antioxidant proteins including superoxide dismutase (SOD) and catalase as well as autophagy-related proteins such as Becin-1, LC3II/I, and Atg7 induced by <span style="font-variant: small-caps;">D</span>-galactose treatment were significantly ameliorated by Fe<sub>3</sub>O<sub>4</sub> nanozymes in the DG of the mouse hippocampus. Furthermore, Fe<sub>3</sub>O<sub>4</sub> nanozyme treatment showed an inhibitory effect against apoptosis in the hippocampus. In conclusion, Fe<sub>3</sub>O<sub>4</sub> nanozymes can relieve neuroblast damage and promote neuroblast differentiation in the hippocampal DG by regulating oxidative stress, apoptosis, and autophagy. |
| first_indexed | 2024-03-09T23:36:20Z |
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| id | doaj.art-ad806ce50d2e4f078c50b7b2c4ff1676 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:36:20Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ad806ce50d2e4f078c50b7b2c4ff16762023-11-23T17:01:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312646310.3390/ijms23126463Fe<sub>3</sub>O<sub>4</sub> Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in <span style="font-variant: small-caps">D</span>-Galactose-Induced Aged MiceZihao Xia0Manman Gao1Peng Sheng2Mengmeng Shen3Lin Zhao4Lizeng Gao5Bingchun Yan6Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou 225001, ChinaJiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou 225001, ChinaJiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou 225001, ChinaJiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou 225001, ChinaJiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou 225001, ChinaCAS Engineering Laboratory for Nanozyme, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaJiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou 225001, ChinaAging is a process associated with blood–brain barrier (BBB) damage and the reduction in neurogenesis, and is the greatest known risk factor for neurodegenerative disorders. However, the effects of Fe<sub>3</sub>O<sub>4</sub> nanozymes on neurogenesis have rarely been studied. This study examined the effects of Fe<sub>3</sub>O<sub>4</sub> nanozymes on neuronal differentiation in the dentate gyrus (DG) and BBB integrity of D-galactose-induced aged mice. Long-term treatment with Fe<sub>3</sub>O<sub>4</sub> nanozymes (10 μg/mL diluted in ddH<sub>2</sub>O daily) markedly increased the doublecortin (DCX) immunoreactivity and decreased BBB injury induced by <span style="font-variant: small-caps;">D</span>-galactose treatment. In addition, the decreases in the levels of antioxidant proteins including superoxide dismutase (SOD) and catalase as well as autophagy-related proteins such as Becin-1, LC3II/I, and Atg7 induced by <span style="font-variant: small-caps;">D</span>-galactose treatment were significantly ameliorated by Fe<sub>3</sub>O<sub>4</sub> nanozymes in the DG of the mouse hippocampus. Furthermore, Fe<sub>3</sub>O<sub>4</sub> nanozyme treatment showed an inhibitory effect against apoptosis in the hippocampus. In conclusion, Fe<sub>3</sub>O<sub>4</sub> nanozymes can relieve neuroblast damage and promote neuroblast differentiation in the hippocampal DG by regulating oxidative stress, apoptosis, and autophagy.https://www.mdpi.com/1422-0067/23/12/6463Fe<sub>3</sub>O<sub>4</sub> nanozymeneuroblast differentiationblood–brain barrierantioxidantaging |
| spellingShingle | Zihao Xia Manman Gao Peng Sheng Mengmeng Shen Lin Zhao Lizeng Gao Bingchun Yan Fe<sub>3</sub>O<sub>4</sub> Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in <span style="font-variant: small-caps">D</span>-Galactose-Induced Aged Mice International Journal of Molecular Sciences Fe<sub>3</sub>O<sub>4</sub> nanozyme neuroblast differentiation blood–brain barrier antioxidant aging |
| title | Fe<sub>3</sub>O<sub>4</sub> Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in <span style="font-variant: small-caps">D</span>-Galactose-Induced Aged Mice |
| title_full | Fe<sub>3</sub>O<sub>4</sub> Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in <span style="font-variant: small-caps">D</span>-Galactose-Induced Aged Mice |
| title_fullStr | Fe<sub>3</sub>O<sub>4</sub> Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in <span style="font-variant: small-caps">D</span>-Galactose-Induced Aged Mice |
| title_full_unstemmed | Fe<sub>3</sub>O<sub>4</sub> Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in <span style="font-variant: small-caps">D</span>-Galactose-Induced Aged Mice |
| title_short | Fe<sub>3</sub>O<sub>4</sub> Nanozymes Improve Neuroblast Differentiation and Blood-Brain Barrier Integrity of the Hippocampal Dentate Gyrus in <span style="font-variant: small-caps">D</span>-Galactose-Induced Aged Mice |
| title_sort | fe sub 3 sub o sub 4 sub nanozymes improve neuroblast differentiation and blood brain barrier integrity of the hippocampal dentate gyrus in span style font variant small caps d span galactose induced aged mice |
| topic | Fe<sub>3</sub>O<sub>4</sub> nanozyme neuroblast differentiation blood–brain barrier antioxidant aging |
| url | https://www.mdpi.com/1422-0067/23/12/6463 |
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