Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site
During the 1960s, small quantities of radioactive materials were co-disposed with chemical waste at the Little Forest Legacy Site (LFLS, Sydney, Australia). The microbial function and population dynamics in a waste trench during a rainfall event have been previously investigated revealing a broad ab...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.732575/full |
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| author | Xabier Vázquez-Campos Andrew S. Kinsela Mark W. Bligh Timothy E. Payne Marc R. Wilkins T. David Waite |
| author_facet | Xabier Vázquez-Campos Andrew S. Kinsela Mark W. Bligh Timothy E. Payne Marc R. Wilkins T. David Waite |
| author_sort | Xabier Vázquez-Campos |
| collection | DOAJ |
| description | During the 1960s, small quantities of radioactive materials were co-disposed with chemical waste at the Little Forest Legacy Site (LFLS, Sydney, Australia). The microbial function and population dynamics in a waste trench during a rainfall event have been previously investigated revealing a broad abundance of candidate and potentially undescribed taxa in this iron-rich, radionuclide-contaminated environment. Applying genome-based metagenomic methods, we recovered 37 refined archaeal MAGs, mainly from undescribed DPANN Archaea lineages without standing in nomenclature and ‘Candidatus Methanoperedenaceae’ (ANME-2D). Within the undescribed DPANN, the newly proposed orders ‘Ca. Gugararchaeales’, ‘Ca. Burarchaeales’ and ‘Ca. Anstonellales’, constitute distinct lineages with a more comprehensive central metabolism and anabolic capabilities within the ‘Ca. Micrarchaeota’ phylum compared to most other DPANN. The analysis of new and extant ‘Ca. Methanoperedens spp.’ MAGs suggests metal ions as the ancestral electron acceptors during the anaerobic oxidation of methane while the respiration of nitrate/nitrite via molybdopterin oxidoreductases would have been a secondary acquisition. The presence of genes for the biosynthesis of polyhydroxyalkanoates in most ‘Ca. Methanoperedens’ also appears to be a widespread characteristic of the genus for carbon accumulation. This work expands our knowledge about the roles of the Archaea at the LFLS, especially, DPANN Archaea and ‘Ca. Methanoperedens’, while exploring their diversity, uniqueness, potential role in elemental cycling, and evolutionary history. |
| first_indexed | 2024-12-16T10:19:45Z |
| format | Article |
| id | doaj.art-ad81c1879b074313ae13d0af7b2f4c10 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T10:19:45Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ad81c1879b074313ae13d0af7b2f4c102022-12-21T22:35:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-10-011210.3389/fmicb.2021.732575732575Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy SiteXabier Vázquez-Campos0Andrew S. Kinsela1Mark W. Bligh2Timothy E. Payne3Marc R. Wilkins4T. David Waite5NSW Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, AustraliaUNSW Water Research Centre, School of Civil and Environmental Engineering, The University of New South Wales, Sydney, NSW, AustraliaUNSW Water Research Centre, School of Civil and Environmental Engineering, The University of New South Wales, Sydney, NSW, AustraliaEnvironmental Research Theme, Australian Nuclear Science and Technology Organisation, Kirrawee DC, NSW, AustraliaNSW Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, AustraliaUNSW Water Research Centre, School of Civil and Environmental Engineering, The University of New South Wales, Sydney, NSW, AustraliaDuring the 1960s, small quantities of radioactive materials were co-disposed with chemical waste at the Little Forest Legacy Site (LFLS, Sydney, Australia). The microbial function and population dynamics in a waste trench during a rainfall event have been previously investigated revealing a broad abundance of candidate and potentially undescribed taxa in this iron-rich, radionuclide-contaminated environment. Applying genome-based metagenomic methods, we recovered 37 refined archaeal MAGs, mainly from undescribed DPANN Archaea lineages without standing in nomenclature and ‘Candidatus Methanoperedenaceae’ (ANME-2D). Within the undescribed DPANN, the newly proposed orders ‘Ca. Gugararchaeales’, ‘Ca. Burarchaeales’ and ‘Ca. Anstonellales’, constitute distinct lineages with a more comprehensive central metabolism and anabolic capabilities within the ‘Ca. Micrarchaeota’ phylum compared to most other DPANN. The analysis of new and extant ‘Ca. Methanoperedens spp.’ MAGs suggests metal ions as the ancestral electron acceptors during the anaerobic oxidation of methane while the respiration of nitrate/nitrite via molybdopterin oxidoreductases would have been a secondary acquisition. The presence of genes for the biosynthesis of polyhydroxyalkanoates in most ‘Ca. Methanoperedens’ also appears to be a widespread characteristic of the genus for carbon accumulation. This work expands our knowledge about the roles of the Archaea at the LFLS, especially, DPANN Archaea and ‘Ca. Methanoperedens’, while exploring their diversity, uniqueness, potential role in elemental cycling, and evolutionary history.https://www.frontiersin.org/articles/10.3389/fmicb.2021.732575/fullArchaeaDPANNmetagenomicsphylogenomics‘Candidatus Methanoperedens’ANME-2d |
| spellingShingle | Xabier Vázquez-Campos Andrew S. Kinsela Mark W. Bligh Timothy E. Payne Marc R. Wilkins T. David Waite Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site Frontiers in Microbiology Archaea DPANN metagenomics phylogenomics ‘Candidatus Methanoperedens’ ANME-2d |
| title | Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site |
| title_full | Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site |
| title_fullStr | Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site |
| title_full_unstemmed | Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site |
| title_short | Genomic Insights Into the Archaea Inhabiting an Australian Radioactive Legacy Site |
| title_sort | genomic insights into the archaea inhabiting an australian radioactive legacy site |
| topic | Archaea DPANN metagenomics phylogenomics ‘Candidatus Methanoperedens’ ANME-2d |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.732575/full |
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