Combining DNA Mini-Barcoding and Species-Specific Primers PCR Technology for Identification of Heosemys grandis
Heosemys grandis, a species of Asian water turtle, that has a wide range of applications in the food and pharmaceutical industries. Since some processed products cannot be identified exclusively by morphological and microscopic identification, a reliable and quick approach to guarantee authenticity...
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| Format: | Article |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Ecology and Evolution |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fevo.2022.822871/full |
| _version_ | 1818353920683016192 |
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| author | Ping Li Dong Li Yuling Hong Mingyang Chen Xia Zhang Liu Hu Chunsheng Liu |
| author_facet | Ping Li Dong Li Yuling Hong Mingyang Chen Xia Zhang Liu Hu Chunsheng Liu |
| author_sort | Ping Li |
| collection | DOAJ |
| description | Heosemys grandis, a species of Asian water turtle, that has a wide range of applications in the food and pharmaceutical industries. Since some processed products cannot be identified exclusively by morphological and microscopic identification, a reliable and quick approach to guarantee authenticity is critical. Thus, we fostered an effective and stable molecular identification system to identify Heosemys grandis based on DNA mini-barcoding and species-specific primers PCR technology. A total of 48 turtle samples from 16 different species were collected. To distinguish Heosemys grandis from its counterfeits, DNA mini-barcoding and a pair of species-specific primers were designed and verified by PCR after analyzing the COI sequences of samples. The results showed that only Heosemys grandis samples could generate a single clear band following amplification using species-specific primers. Employing DNA mini-barcoding to amplify samples can verify authenticity by sequence alignment. These findings indicated that species-specific primers PCR technology combined with DNA mini-barcoding could accurately detect the authenticity of Heosemys grandis. This technology broadens the application of molecular biology techniques in the food and pharmaceutical industries. It provides a reliable and convenient method for identifying raw materials to standardize the market and protect customers’ rights and interests. |
| first_indexed | 2024-12-13T19:17:12Z |
| format | Article |
| id | doaj.art-ad83fa1e10434f03ac0a2b75091d0d08 |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-12-13T19:17:12Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-ad83fa1e10434f03ac0a2b75091d0d082022-12-21T23:34:15ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2022-04-011010.3389/fevo.2022.822871822871Combining DNA Mini-Barcoding and Species-Specific Primers PCR Technology for Identification of Heosemys grandisPing Li0Dong Li1Yuling Hong2Mingyang Chen3Xia Zhang4Liu Hu5Chunsheng Liu6School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaZhejiang Yangshengtang Institute of Natural Medication Co., Ltd., Hangzhou, ChinaYangshengtang Pharmaceutical Co., Ltd., Hangzhou, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaZhejiang Yangshengtang Institute of Natural Medication Co., Ltd., Hangzhou, ChinaZhejiang Yangshengtang Institute of Natural Medication Co., Ltd., Hangzhou, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaHeosemys grandis, a species of Asian water turtle, that has a wide range of applications in the food and pharmaceutical industries. Since some processed products cannot be identified exclusively by morphological and microscopic identification, a reliable and quick approach to guarantee authenticity is critical. Thus, we fostered an effective and stable molecular identification system to identify Heosemys grandis based on DNA mini-barcoding and species-specific primers PCR technology. A total of 48 turtle samples from 16 different species were collected. To distinguish Heosemys grandis from its counterfeits, DNA mini-barcoding and a pair of species-specific primers were designed and verified by PCR after analyzing the COI sequences of samples. The results showed that only Heosemys grandis samples could generate a single clear band following amplification using species-specific primers. Employing DNA mini-barcoding to amplify samples can verify authenticity by sequence alignment. These findings indicated that species-specific primers PCR technology combined with DNA mini-barcoding could accurately detect the authenticity of Heosemys grandis. This technology broadens the application of molecular biology techniques in the food and pharmaceutical industries. It provides a reliable and convenient method for identifying raw materials to standardize the market and protect customers’ rights and interests.https://www.frontiersin.org/articles/10.3389/fevo.2022.822871/fullHeosemys grandisCOIDNA mini-barcodingspecies-specific primersidentification |
| spellingShingle | Ping Li Dong Li Yuling Hong Mingyang Chen Xia Zhang Liu Hu Chunsheng Liu Combining DNA Mini-Barcoding and Species-Specific Primers PCR Technology for Identification of Heosemys grandis Frontiers in Ecology and Evolution Heosemys grandis COI DNA mini-barcoding species-specific primers identification |
| title | Combining DNA Mini-Barcoding and Species-Specific Primers PCR Technology for Identification of Heosemys grandis |
| title_full | Combining DNA Mini-Barcoding and Species-Specific Primers PCR Technology for Identification of Heosemys grandis |
| title_fullStr | Combining DNA Mini-Barcoding and Species-Specific Primers PCR Technology for Identification of Heosemys grandis |
| title_full_unstemmed | Combining DNA Mini-Barcoding and Species-Specific Primers PCR Technology for Identification of Heosemys grandis |
| title_short | Combining DNA Mini-Barcoding and Species-Specific Primers PCR Technology for Identification of Heosemys grandis |
| title_sort | combining dna mini barcoding and species specific primers pcr technology for identification of heosemys grandis |
| topic | Heosemys grandis COI DNA mini-barcoding species-specific primers identification |
| url | https://www.frontiersin.org/articles/10.3389/fevo.2022.822871/full |
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