Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice
IntroductionCognitive impairment includes the abnormality of learning, memory and judgment, resulting in severe learning and memory impairment and social activity impairment, which greatly affects the life quality of individuals. However, the specific mechanisms underlying cognitive impairment in di...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2023.1139737/full |
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| author | Ying-Ke Jiang Fei-Yuan Dong Yi-Bei Dong Xin-Yi Zhu Lu-Hui Pan Lin-Bo Hu Le Xu Xiao-Fan Xu Li-Min Xu Xiao-Qin Zhang |
| author_facet | Ying-Ke Jiang Fei-Yuan Dong Yi-Bei Dong Xin-Yi Zhu Lu-Hui Pan Lin-Bo Hu Le Xu Xiao-Fan Xu Li-Min Xu Xiao-Qin Zhang |
| author_sort | Ying-Ke Jiang |
| collection | DOAJ |
| description | IntroductionCognitive impairment includes the abnormality of learning, memory and judgment, resulting in severe learning and memory impairment and social activity impairment, which greatly affects the life quality of individuals. However, the specific mechanisms underlying cognitive impairment in different behavioral paradigms remain to be elucidated.MethodsThe study utilized two behavioral paradigms, novel location recognition (NLR) and novel object recognition (NOR), to investigate the brain regions involved in cognitive function. These tests comprised two phases: mice were presented with two identical objects for familiarization during the training phase, and a novel (experiment) or familiar (control) object/location was presented during testing. Immunostaining quantification of c-Fos, an immediate early gene used as a neuronal activity marker, was performed in eight different brain regions after the NLR or NOR test.ResultsThe number of c-Fos-positive cells was significantly higher in the dorsal part of the lateral septal nucleus (LSD) in the NLR and dentate gyrus (DG) in the NOR experiment group than in the control group. We further bilaterally lesioned these regions using excitotoxic ibotenic acid and replenished the damaged areas using an antisense oligonucleotide (ASO) strategy.DiscussionThese data reinforced the importance of LSD and DG in regulating spatial and object recognition memory, respectively. Thus, the study provides insight into the roles of these brain regions and suggests potential intervention targets for impaired spatial and object recognition memory. |
| first_indexed | 2024-04-09T20:16:47Z |
| format | Article |
| id | doaj.art-ad84ea275c674eb1a7f3b57a54966b62 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-09T20:16:47Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-ad84ea275c674eb1a7f3b57a54966b622023-03-31T07:11:08ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532023-03-011710.3389/fnbeh.2023.11397371139737Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in miceYing-Ke Jiang0Fei-Yuan Dong1Yi-Bei Dong2Xin-Yi Zhu3Lu-Hui Pan4Lin-Bo Hu5Le Xu6Xiao-Fan Xu7Li-Min Xu8Xiao-Qin Zhang9Department of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang, ChinaNingbo Women and Children’s Hospital, Ningbo, Zhejiang, ChinaDepartment of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang, ChinaIntroductionCognitive impairment includes the abnormality of learning, memory and judgment, resulting in severe learning and memory impairment and social activity impairment, which greatly affects the life quality of individuals. However, the specific mechanisms underlying cognitive impairment in different behavioral paradigms remain to be elucidated.MethodsThe study utilized two behavioral paradigms, novel location recognition (NLR) and novel object recognition (NOR), to investigate the brain regions involved in cognitive function. These tests comprised two phases: mice were presented with two identical objects for familiarization during the training phase, and a novel (experiment) or familiar (control) object/location was presented during testing. Immunostaining quantification of c-Fos, an immediate early gene used as a neuronal activity marker, was performed in eight different brain regions after the NLR or NOR test.ResultsThe number of c-Fos-positive cells was significantly higher in the dorsal part of the lateral septal nucleus (LSD) in the NLR and dentate gyrus (DG) in the NOR experiment group than in the control group. We further bilaterally lesioned these regions using excitotoxic ibotenic acid and replenished the damaged areas using an antisense oligonucleotide (ASO) strategy.DiscussionThese data reinforced the importance of LSD and DG in regulating spatial and object recognition memory, respectively. Thus, the study provides insight into the roles of these brain regions and suggests potential intervention targets for impaired spatial and object recognition memory.https://www.frontiersin.org/articles/10.3389/fnbeh.2023.1139737/fulllateral septal nucleus (LSD)dorsal partdentate gyrusrecognition memoryc-Foslesion |
| spellingShingle | Ying-Ke Jiang Fei-Yuan Dong Yi-Bei Dong Xin-Yi Zhu Lu-Hui Pan Lin-Bo Hu Le Xu Xiao-Fan Xu Li-Min Xu Xiao-Qin Zhang Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice Frontiers in Behavioral Neuroscience lateral septal nucleus (LSD) dorsal part dentate gyrus recognition memory c-Fos lesion |
| title | Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice |
| title_full | Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice |
| title_fullStr | Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice |
| title_full_unstemmed | Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice |
| title_short | Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice |
| title_sort | lateral septal nucleus dorsal part and dentate gyrus are necessary for spatial and object recognition memory respectively in mice |
| topic | lateral septal nucleus (LSD) dorsal part dentate gyrus recognition memory c-Fos lesion |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2023.1139737/full |
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