Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index
Abstract Background Adipokines such as leptin, visfatin and chemerin play a pivotal role not only in the pathogenesis of excessive weight gain but also impact on hepatic metabolism. However, alterations in the production of these peptides in the liver of overweight individuals have not been fully el...
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BMC
2022-05-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-022-02299-6 |
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| author | I. Kasacka Ż. Piotrowska N. Domian A. Lewandowska M. Acewicz |
| author_facet | I. Kasacka Ż. Piotrowska N. Domian A. Lewandowska M. Acewicz |
| author_sort | I. Kasacka |
| collection | DOAJ |
| description | Abstract Background Adipokines such as leptin, visfatin and chemerin play a pivotal role not only in the pathogenesis of excessive weight gain but also impact on hepatic metabolism. However, alterations in the production of these peptides in the liver of overweight individuals have not been fully elucidated yet. The aim of the study was to evaluate changes in leptin, visfatin and chemerin biosynthesis in the liver of men with different BMI. Methods Fourteen adult men without symptoms from the digestive system were recruited. Research material consisted of liver samples. Study participants were divided into two groups: lean (BMI ≤ 25 kg/m2) and overweight subjects (BMI > 25 kg/m2). Paraffin liver sections were processed by immunohistochemistry for detection of leptin, visfatin and chemerin. Hepatic expression of leptin, visfatin and chemerin genes was determined by qRT-PCR method. Results Increased immunoreactivity for leptin and chemerin, and decreased immunoreaction for visfatin were observed in the liver of overweight men in comparison to lean subjects. Overweight subjects with hepatic steatosis displayed increased immunoreactivity for leptin and weaker immunoreaction against visfatin and chemerin in the liver, compared to individuals with normal organ structure. Expression of leptin and chemerin was enhanced in the liver of overweight individuals, with the highest expression observed in subjects with hepatic steatosis. Conversely, expression of visfatin in the male liver was decreased in overweight subjects and those with and liver steatosis. Conclusions The present study proves that the expression of leptin, visfatin and chemerin in the male liver is altered in overweight individuals. Our report also indicates the potential importance of these peptides in hepatic steatosis associated with overweight. |
| first_indexed | 2024-12-12T03:18:24Z |
| format | Article |
| id | doaj.art-ad885cc3fa824649ac577b4149139a5d |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-12-12T03:18:24Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-ad885cc3fa824649ac577b4149139a5d2022-12-22T00:40:13ZengBMCBMC Gastroenterology1471-230X2022-05-0122111010.1186/s12876-022-02299-6Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass indexI. Kasacka0Ż. Piotrowska1N. Domian2A. Lewandowska3M. Acewicz4Department of Histology and Cytophysiology, Medical University of BiałystokDepartment of Histology and Cytophysiology, Medical University of BiałystokDepartment of Histology and Cytophysiology, Medical University of BiałystokDepartment of Histology and Cytophysiology, Medical University of BiałystokDepartment of Histology and Cytophysiology, Medical University of BiałystokAbstract Background Adipokines such as leptin, visfatin and chemerin play a pivotal role not only in the pathogenesis of excessive weight gain but also impact on hepatic metabolism. However, alterations in the production of these peptides in the liver of overweight individuals have not been fully elucidated yet. The aim of the study was to evaluate changes in leptin, visfatin and chemerin biosynthesis in the liver of men with different BMI. Methods Fourteen adult men without symptoms from the digestive system were recruited. Research material consisted of liver samples. Study participants were divided into two groups: lean (BMI ≤ 25 kg/m2) and overweight subjects (BMI > 25 kg/m2). Paraffin liver sections were processed by immunohistochemistry for detection of leptin, visfatin and chemerin. Hepatic expression of leptin, visfatin and chemerin genes was determined by qRT-PCR method. Results Increased immunoreactivity for leptin and chemerin, and decreased immunoreaction for visfatin were observed in the liver of overweight men in comparison to lean subjects. Overweight subjects with hepatic steatosis displayed increased immunoreactivity for leptin and weaker immunoreaction against visfatin and chemerin in the liver, compared to individuals with normal organ structure. Expression of leptin and chemerin was enhanced in the liver of overweight individuals, with the highest expression observed in subjects with hepatic steatosis. Conversely, expression of visfatin in the male liver was decreased in overweight subjects and those with and liver steatosis. Conclusions The present study proves that the expression of leptin, visfatin and chemerin in the male liver is altered in overweight individuals. Our report also indicates the potential importance of these peptides in hepatic steatosis associated with overweight.https://doi.org/10.1186/s12876-022-02299-6LeptinVisfatinChemerinLiverHumanOverweight |
| spellingShingle | I. Kasacka Ż. Piotrowska N. Domian A. Lewandowska M. Acewicz Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index BMC Gastroenterology Leptin Visfatin Chemerin Liver Human Overweight |
| title | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
| title_full | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
| title_fullStr | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
| title_full_unstemmed | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
| title_short | Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index |
| title_sort | immunohistochemical identification and assessment of the location of leptin visfatin and chemerin in the liver of men with different body mass index |
| topic | Leptin Visfatin Chemerin Liver Human Overweight |
| url | https://doi.org/10.1186/s12876-022-02299-6 |
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