Identification of quorum sensing-regulated Vibrio fortis as potential pathogenic bacteria for coral bleaching and the effects on the microbial shift
Coastal pollution, global warming, ocean acidification, and other reasons lead to the imbalance of the coral reef ecosystem, resulting in the increasingly serious problem of coral degradation. Coral bleaching is often accompanied by structural abnormalities of coral symbiotic microbiota, among which...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1116737/full |
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| author | Xiaohui Sun Yan Li Qian Yang Han Zhang Nuo Xu Zheng Tang Shishi Wu Yusheng Jiang Hala F. Mohamed Hala F. Mohamed Danyun Ou Danyun Ou Xinqing Zheng Xinqing Zheng Xinqing Zheng |
| author_facet | Xiaohui Sun Yan Li Qian Yang Han Zhang Nuo Xu Zheng Tang Shishi Wu Yusheng Jiang Hala F. Mohamed Hala F. Mohamed Danyun Ou Danyun Ou Xinqing Zheng Xinqing Zheng Xinqing Zheng |
| author_sort | Xiaohui Sun |
| collection | DOAJ |
| description | Coastal pollution, global warming, ocean acidification, and other reasons lead to the imbalance of the coral reef ecosystem, resulting in the increasingly serious problem of coral degradation. Coral bleaching is often accompanied by structural abnormalities of coral symbiotic microbiota, among which Vibrio is highly concerned. In this study, Vibrio fortis S10-1 (MCCC 1H00104), isolated from sea cucumber, was used for the bacterial infection on coral Seriatopora guttatus and Pocillopora damicornis. The infection of S10-1 led to coral bleaching and a significant reduction of photosynthetic function in coral holobiont, and the pathogenicity of V. fortis was regulated by quorum sensing. Meanwhile, Vibrio infection also caused a shift of coral symbiotic microbial community, with significantly increased abundant Proteobacteria and Actinobacteria and significantly reduced abundant Firmicutes; on genus level, the abundance of Bacillus decreased significantly and the abundance of Rhodococcus, Ralstonia, and Burkholderia–Caballeronia–Paraburkholderia increased significantly; S10-1 infection also significantly impacted the water quality in the micro-ecosystem. In contrast, S10-1 infection showed less effect on the microbial community of the live stone, which reflected that the microbes in the epiphytic environment of the live stone might have a stronger ability of self-regulation; the algal symbionts mainly consisted of Cladocopium sp. and showed no significant effect by the Vibrio infection. This study verified that V. fortis is the primary pathogenic bacterium causing coral bleaching, revealed changes in the microbial community caused by its infection, provided strong evidence for the “bacterial bleaching” hypothesis, and provided an experimental experience for the exploration of the interaction mechanism among microbial communities, especially coral-associated Vibrio in the coral ecosystem, and potential probiotic strategy or QS regulation on further coral disease control. |
| first_indexed | 2024-04-10T17:44:35Z |
| format | Article |
| id | doaj.art-ad8ac7b236754b05acf46306bdddd4e3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T17:44:35Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ad8ac7b236754b05acf46306bdddd4e32023-02-03T05:32:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.11167371116737Identification of quorum sensing-regulated Vibrio fortis as potential pathogenic bacteria for coral bleaching and the effects on the microbial shiftXiaohui Sun0Yan Li1Qian Yang2Han Zhang3Nuo Xu4Zheng Tang5Shishi Wu6Yusheng Jiang7Hala F. Mohamed8Hala F. Mohamed9Danyun Ou10Danyun Ou11Xinqing Zheng12Xinqing Zheng13Xinqing Zheng14College of Chemical Engineering, Huaqiao University, Xiamen, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaCollege of Chemical Engineering, Huaqiao University, Xiamen, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaCollege of Chemical Engineering, Huaqiao University, Xiamen, ChinaCollege of Chemical Engineering, Huaqiao University, Xiamen, ChinaCollege of Chemical Engineering, Huaqiao University, Xiamen, ChinaCollege of Chemical Engineering, Huaqiao University, Xiamen, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaBotany and Microbiology Department (Girls Branch), Faculty of Science, Al-Azhar University, Cairo, EgyptThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaKey Laboratory of Marine Ecological Conservation and Restoration, Ministry of Natural Resources, Xiamen, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaKey Laboratory of Marine Ecological Conservation and Restoration, Ministry of Natural Resources, Xiamen, ChinaObservation and Research Station of Coastal Wetland Ecosystem in Beibu Gulf, Ministry of Natural Resources, Xiamen, ChinaCoastal pollution, global warming, ocean acidification, and other reasons lead to the imbalance of the coral reef ecosystem, resulting in the increasingly serious problem of coral degradation. Coral bleaching is often accompanied by structural abnormalities of coral symbiotic microbiota, among which Vibrio is highly concerned. In this study, Vibrio fortis S10-1 (MCCC 1H00104), isolated from sea cucumber, was used for the bacterial infection on coral Seriatopora guttatus and Pocillopora damicornis. The infection of S10-1 led to coral bleaching and a significant reduction of photosynthetic function in coral holobiont, and the pathogenicity of V. fortis was regulated by quorum sensing. Meanwhile, Vibrio infection also caused a shift of coral symbiotic microbial community, with significantly increased abundant Proteobacteria and Actinobacteria and significantly reduced abundant Firmicutes; on genus level, the abundance of Bacillus decreased significantly and the abundance of Rhodococcus, Ralstonia, and Burkholderia–Caballeronia–Paraburkholderia increased significantly; S10-1 infection also significantly impacted the water quality in the micro-ecosystem. In contrast, S10-1 infection showed less effect on the microbial community of the live stone, which reflected that the microbes in the epiphytic environment of the live stone might have a stronger ability of self-regulation; the algal symbionts mainly consisted of Cladocopium sp. and showed no significant effect by the Vibrio infection. This study verified that V. fortis is the primary pathogenic bacterium causing coral bleaching, revealed changes in the microbial community caused by its infection, provided strong evidence for the “bacterial bleaching” hypothesis, and provided an experimental experience for the exploration of the interaction mechanism among microbial communities, especially coral-associated Vibrio in the coral ecosystem, and potential probiotic strategy or QS regulation on further coral disease control.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1116737/fullcoral bleachingVibrio fortissymbiotic microbesbacterial infectionmicrobial shift |
| spellingShingle | Xiaohui Sun Yan Li Qian Yang Han Zhang Nuo Xu Zheng Tang Shishi Wu Yusheng Jiang Hala F. Mohamed Hala F. Mohamed Danyun Ou Danyun Ou Xinqing Zheng Xinqing Zheng Xinqing Zheng Identification of quorum sensing-regulated Vibrio fortis as potential pathogenic bacteria for coral bleaching and the effects on the microbial shift Frontiers in Microbiology coral bleaching Vibrio fortis symbiotic microbes bacterial infection microbial shift |
| title | Identification of quorum sensing-regulated Vibrio fortis as potential pathogenic bacteria for coral bleaching and the effects on the microbial shift |
| title_full | Identification of quorum sensing-regulated Vibrio fortis as potential pathogenic bacteria for coral bleaching and the effects on the microbial shift |
| title_fullStr | Identification of quorum sensing-regulated Vibrio fortis as potential pathogenic bacteria for coral bleaching and the effects on the microbial shift |
| title_full_unstemmed | Identification of quorum sensing-regulated Vibrio fortis as potential pathogenic bacteria for coral bleaching and the effects on the microbial shift |
| title_short | Identification of quorum sensing-regulated Vibrio fortis as potential pathogenic bacteria for coral bleaching and the effects on the microbial shift |
| title_sort | identification of quorum sensing regulated vibrio fortis as potential pathogenic bacteria for coral bleaching and the effects on the microbial shift |
| topic | coral bleaching Vibrio fortis symbiotic microbes bacterial infection microbial shift |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1116737/full |
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