Investigating the Relationship between Transverse Relaxation Rate () and Interecho Time in MagA-Expressing, Iron-Labeled Cells
Reporter gene–based labeling of cells with iron is an emerging method of providing magnetic resonance imaging contrast for long-term cell tracking and monitoring cellular activities. This report investigates 9.4 T nuclear magnetic resonance properties of mammalian cells overexpressing MagA, a putati...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publications
2015-12-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2015.00027 |
| _version_ | 1797288320831586304 |
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| author | Casey Y. Lee R. Terry Thompson Frank S. Prato Donna E. Goldhawk Neil Gelman |
| author_facet | Casey Y. Lee R. Terry Thompson Frank S. Prato Donna E. Goldhawk Neil Gelman |
| author_sort | Casey Y. Lee |
| collection | DOAJ |
| description | Reporter gene–based labeling of cells with iron is an emerging method of providing magnetic resonance imaging contrast for long-term cell tracking and monitoring cellular activities. This report investigates 9.4 T nuclear magnetic resonance properties of mammalian cells overexpressing MagA, a putative iron transport protein from magnetotactic bacteria. MagA-expressing MDA-MB-435 cells were cultured in the presence and absence of iron supplementation and compared to the untransfected control. The relationship between the transverse relaxation rate ( R2 ) and interecho time was investigated using the Carr-Purcell-Meiboom-Gill sequence. This relationship was analyzed using a model based on water diffusion in weak magnetic field inhomogeneities (Jensen-Chandra model) as well as a fast-exchange model (Luz-Meiboom model). Increases in R2 with increasing interecho time were larger in the iron-supplemented, MagA-expressing cells compared to other cells. The dependence of R2 on interecho time in these iron-supplemented, MagA-expressing cells was better represented by the Jensen-Chandra model compared to the Luz-Meiboom model, whereas the Luz-Meiboom model performed better for the remaining cell types. Our findings provide an estimate of the distance scale of microscopic magnetic field variations in MagA-expressing cells, which is thought to be related to the size of iron-containing vesicles. |
| first_indexed | 2024-03-07T18:47:37Z |
| format | Article |
| id | doaj.art-ad91a4854a9e4242ba96650cc5d99462 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T18:47:37Z |
| publishDate | 2015-12-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-ad91a4854a9e4242ba96650cc5d994622024-03-02T02:17:50ZengSAGE PublicationsMolecular Imaging1536-01212015-12-011410.2310/7290.2015.0002710.2310_7290.2015.00027Investigating the Relationship between Transverse Relaxation Rate () and Interecho Time in MagA-Expressing, Iron-Labeled CellsCasey Y. LeeR. Terry ThompsonFrank S. PratoDonna E. GoldhawkNeil GelmanReporter gene–based labeling of cells with iron is an emerging method of providing magnetic resonance imaging contrast for long-term cell tracking and monitoring cellular activities. This report investigates 9.4 T nuclear magnetic resonance properties of mammalian cells overexpressing MagA, a putative iron transport protein from magnetotactic bacteria. MagA-expressing MDA-MB-435 cells were cultured in the presence and absence of iron supplementation and compared to the untransfected control. The relationship between the transverse relaxation rate ( R2 ) and interecho time was investigated using the Carr-Purcell-Meiboom-Gill sequence. This relationship was analyzed using a model based on water diffusion in weak magnetic field inhomogeneities (Jensen-Chandra model) as well as a fast-exchange model (Luz-Meiboom model). Increases in R2 with increasing interecho time were larger in the iron-supplemented, MagA-expressing cells compared to other cells. The dependence of R2 on interecho time in these iron-supplemented, MagA-expressing cells was better represented by the Jensen-Chandra model compared to the Luz-Meiboom model, whereas the Luz-Meiboom model performed better for the remaining cell types. Our findings provide an estimate of the distance scale of microscopic magnetic field variations in MagA-expressing cells, which is thought to be related to the size of iron-containing vesicles.https://doi.org/10.2310/7290.2015.00027 |
| spellingShingle | Casey Y. Lee R. Terry Thompson Frank S. Prato Donna E. Goldhawk Neil Gelman Investigating the Relationship between Transverse Relaxation Rate () and Interecho Time in MagA-Expressing, Iron-Labeled Cells Molecular Imaging |
| title | Investigating the Relationship between Transverse Relaxation Rate () and Interecho Time in MagA-Expressing, Iron-Labeled Cells |
| title_full | Investigating the Relationship between Transverse Relaxation Rate () and Interecho Time in MagA-Expressing, Iron-Labeled Cells |
| title_fullStr | Investigating the Relationship between Transverse Relaxation Rate () and Interecho Time in MagA-Expressing, Iron-Labeled Cells |
| title_full_unstemmed | Investigating the Relationship between Transverse Relaxation Rate () and Interecho Time in MagA-Expressing, Iron-Labeled Cells |
| title_short | Investigating the Relationship between Transverse Relaxation Rate () and Interecho Time in MagA-Expressing, Iron-Labeled Cells |
| title_sort | investigating the relationship between transverse relaxation rate and interecho time in maga expressing iron labeled cells |
| url | https://doi.org/10.2310/7290.2015.00027 |
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