Hepatic autotaxin overexpression in infants with biliary atresia
Background Autotaxin (ATX) is a secreted glycoprotein that is involved in the development of hepatic fibrogenesis via the enzymatic production of lysophosphatidic acid. The aim of this study was to investigate hepatic expression of ATX in biliary atresia (BA) compared with non-BA liver controls and...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2018-07-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/5224.pdf |
| _version_ | 1797418820914118656 |
|---|---|
| author | Wanvisa Udomsinprasert Paisarn Vejchapipat Naruemon Klaikeaw Voranush Chongsrisawat Yong Poovorawan Sittisak Honsawek |
| author_facet | Wanvisa Udomsinprasert Paisarn Vejchapipat Naruemon Klaikeaw Voranush Chongsrisawat Yong Poovorawan Sittisak Honsawek |
| author_sort | Wanvisa Udomsinprasert |
| collection | DOAJ |
| description | Background Autotaxin (ATX) is a secreted glycoprotein that is involved in the development of hepatic fibrogenesis via the enzymatic production of lysophosphatidic acid. The aim of this study was to investigate hepatic expression of ATX in biliary atresia (BA) compared with non-BA liver controls and to examine the association between ATX expression and clinical outcome in BA. Methods Liver specimens from BA infants (n = 20) were compared with samples from infants who underwent liver biopsy for reasons other than BA (n = 14) and served as controls. Relative mRNA and protein expression of ATX were quantified using real-time polymerase chain reaction (PCR) and immunohistochemistry. Masson’s Trichrome staining was performed to determine the degree of liver fibrosis. Results Quantitative real-time PCR demonstrated overexpression of ATX mRNA in BA livers. In immunohistochemical evaluation, ATX was positively stained on the hepatic parenchyma and the biliary epithelium in BA patients, as compared to non-BA controls. The immunostaining score of ATX in BA livers was also significantly higher than that observed in non-BA livers (P < 0.001). Subgroup analysis revealed that ATX expression in the patients with poor outcomes was significantly greater than in those with good outcomes (P = 0.03). Additionally, there was a positive correlation between hepatic ATX expression and Metavir fibrosis stage in BA livers (r = 0.79, P < 0.001). Discussion This study found that mRNA and protein expression of ATX were increased in BA livers. High hepatic ATX expression at the time of Kasai operation was associated with liver fibrosis and outcome in BA, suggesting that ATX may serve a role as a promising biomarker of the prognosis in biliary atresia. |
| first_indexed | 2024-03-09T06:39:31Z |
| format | Article |
| id | doaj.art-ad9323bf0cf84461a66df1871130791f |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:39:31Z |
| publishDate | 2018-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-ad9323bf0cf84461a66df1871130791f2023-12-03T10:54:04ZengPeerJ Inc.PeerJ2167-83592018-07-016e522410.7717/peerj.5224Hepatic autotaxin overexpression in infants with biliary atresiaWanvisa Udomsinprasert0Paisarn Vejchapipat1Naruemon Klaikeaw2Voranush Chongsrisawat3Yong Poovorawan4Sittisak Honsawek5Osteoarthritis and Musculoskeleton Research Unit, Department of Biochemistry, Faculty of Medicine, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Chulalongkorn University, Bangkok, ThailandDepartment of Surgery, Faculty of Medicine, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Chulalongkorn University, Bangkok, ThailandDepartment of Pathology, Faculty of Medicine, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, King Chulalongkorn Memorial Hospital, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, King Chulalongkorn Memorial Hospital, Chulalongkorn University, Bangkok, ThailandOsteoarthritis and Musculoskeleton Research Unit, Department of Biochemistry, Faculty of Medicine, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Chulalongkorn University, Bangkok, ThailandBackground Autotaxin (ATX) is a secreted glycoprotein that is involved in the development of hepatic fibrogenesis via the enzymatic production of lysophosphatidic acid. The aim of this study was to investigate hepatic expression of ATX in biliary atresia (BA) compared with non-BA liver controls and to examine the association between ATX expression and clinical outcome in BA. Methods Liver specimens from BA infants (n = 20) were compared with samples from infants who underwent liver biopsy for reasons other than BA (n = 14) and served as controls. Relative mRNA and protein expression of ATX were quantified using real-time polymerase chain reaction (PCR) and immunohistochemistry. Masson’s Trichrome staining was performed to determine the degree of liver fibrosis. Results Quantitative real-time PCR demonstrated overexpression of ATX mRNA in BA livers. In immunohistochemical evaluation, ATX was positively stained on the hepatic parenchyma and the biliary epithelium in BA patients, as compared to non-BA controls. The immunostaining score of ATX in BA livers was also significantly higher than that observed in non-BA livers (P < 0.001). Subgroup analysis revealed that ATX expression in the patients with poor outcomes was significantly greater than in those with good outcomes (P = 0.03). Additionally, there was a positive correlation between hepatic ATX expression and Metavir fibrosis stage in BA livers (r = 0.79, P < 0.001). Discussion This study found that mRNA and protein expression of ATX were increased in BA livers. High hepatic ATX expression at the time of Kasai operation was associated with liver fibrosis and outcome in BA, suggesting that ATX may serve a role as a promising biomarker of the prognosis in biliary atresia.https://peerj.com/articles/5224.pdfBiliary atresiaAutotaxinImmunohistochemistryQuantitative real-time polymerase chain reactionLiver |
| spellingShingle | Wanvisa Udomsinprasert Paisarn Vejchapipat Naruemon Klaikeaw Voranush Chongsrisawat Yong Poovorawan Sittisak Honsawek Hepatic autotaxin overexpression in infants with biliary atresia PeerJ Biliary atresia Autotaxin Immunohistochemistry Quantitative real-time polymerase chain reaction Liver |
| title | Hepatic autotaxin overexpression in infants with biliary atresia |
| title_full | Hepatic autotaxin overexpression in infants with biliary atresia |
| title_fullStr | Hepatic autotaxin overexpression in infants with biliary atresia |
| title_full_unstemmed | Hepatic autotaxin overexpression in infants with biliary atresia |
| title_short | Hepatic autotaxin overexpression in infants with biliary atresia |
| title_sort | hepatic autotaxin overexpression in infants with biliary atresia |
| topic | Biliary atresia Autotaxin Immunohistochemistry Quantitative real-time polymerase chain reaction Liver |
| url | https://peerj.com/articles/5224.pdf |
| work_keys_str_mv | AT wanvisaudomsinprasert hepaticautotaxinoverexpressionininfantswithbiliaryatresia AT paisarnvejchapipat hepaticautotaxinoverexpressionininfantswithbiliaryatresia AT naruemonklaikeaw hepaticautotaxinoverexpressionininfantswithbiliaryatresia AT voranushchongsrisawat hepaticautotaxinoverexpressionininfantswithbiliaryatresia AT yongpoovorawan hepaticautotaxinoverexpressionininfantswithbiliaryatresia AT sittisakhonsawek hepaticautotaxinoverexpressionininfantswithbiliaryatresia |