Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced inflammation in human retinal endothelial cells
<b>AIM:</b> To investigate the mechanism underlying the anti-inflammatory effects of epigallocatechin-3-gallate (EGCG) in lipopolysaccharide (LPS)-stimulated human retinal endothelial cells (HRECs).<b>METHODS:</b> HRECs pre-treated with EGCG (0-100 μmol/L) were stimulated wit...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2014-06-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067650/ |
| _version_ | 1818603712931692544 |
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| author | Hui-Yan Zhang Jian-Yong Wang Hang-Ping Yao |
| author_facet | Hui-Yan Zhang Jian-Yong Wang Hang-Ping Yao |
| author_sort | Hui-Yan Zhang |
| collection | DOAJ |
| description | <b>AIM:</b> To investigate the mechanism underlying the anti-inflammatory effects of epigallocatechin-3-gallate (EGCG) in lipopolysaccharide (LPS)-stimulated human retinal endothelial cells (HRECs).<b>METHODS:</b> HRECs pre-treated with EGCG (0-100 μmol/L) were stimulated with LPS (250 ng/mL). Levels of tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor (VEGF), monocyte chemotactic protein-1 (MCP-1) and nitric oxide (NO) in the supernatants were determined by enzyme-linked immunosorbent assay (ELISA) and Griess assay. The protein expression of phosphorylated extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen-activated protein kinases (p38) were determined by Western blot analysis.<b>RESULTS:</b> EGCG pre-treatment significantly inhibited the secretion of TNF-α, VEGF, MCP-1 and NO in LPS-stimulated HRECs. Moreover, EGCG effectively attenuated LPS-induced activation and phosphorylation of ERK1/2 and p38 in HRECs in a dose-dependent manner.<b>CONCLUSION:</b> EGCG exhibited inhibitory effects on LPS-induced pro-inflammatory cytokines production by modulating ERK1/2 and p38 pathways in HRECs, suggesting EGCG as a potential candidate for anti-inflammatory intervention. |
| first_indexed | 2024-12-16T13:27:32Z |
| format | Article |
| id | doaj.art-ad95ed9bd3a5431788ce3efc564d30db |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-16T13:27:32Z |
| publishDate | 2014-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-ad95ed9bd3a5431788ce3efc564d30db2022-12-21T22:30:10ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982014-06-017340841210.3980/j.issn.2222-3959.2014.03.04Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced inflammation in human retinal endothelial cellsHui-Yan Zhang0Jian-Yong Wang1Hang-Ping Yao2Hangzhou Vocational & Technical College, Hangzhou 310018, Zhejiang Province, China<br>Department of Ophthalmology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China<br>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China<b>AIM:</b> To investigate the mechanism underlying the anti-inflammatory effects of epigallocatechin-3-gallate (EGCG) in lipopolysaccharide (LPS)-stimulated human retinal endothelial cells (HRECs).<b>METHODS:</b> HRECs pre-treated with EGCG (0-100 μmol/L) were stimulated with LPS (250 ng/mL). Levels of tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor (VEGF), monocyte chemotactic protein-1 (MCP-1) and nitric oxide (NO) in the supernatants were determined by enzyme-linked immunosorbent assay (ELISA) and Griess assay. The protein expression of phosphorylated extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen-activated protein kinases (p38) were determined by Western blot analysis.<b>RESULTS:</b> EGCG pre-treatment significantly inhibited the secretion of TNF-α, VEGF, MCP-1 and NO in LPS-stimulated HRECs. Moreover, EGCG effectively attenuated LPS-induced activation and phosphorylation of ERK1/2 and p38 in HRECs in a dose-dependent manner.<b>CONCLUSION:</b> EGCG exhibited inhibitory effects on LPS-induced pro-inflammatory cytokines production by modulating ERK1/2 and p38 pathways in HRECs, suggesting EGCG as a potential candidate for anti-inflammatory intervention.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067650/epigallocatechin-3-gallatehuman retinal endothelial cellsinflammatory factorsinflammatory factors |
| spellingShingle | Hui-Yan Zhang Jian-Yong Wang Hang-Ping Yao Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced inflammation in human retinal endothelial cells International Journal of Ophthalmology epigallocatechin-3-gallate human retinal endothelial cells inflammatory factors inflammatory factors |
| title | Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced inflammation in human retinal endothelial cells |
| title_full | Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced inflammation in human retinal endothelial cells |
| title_fullStr | Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced inflammation in human retinal endothelial cells |
| title_full_unstemmed | Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced inflammation in human retinal endothelial cells |
| title_short | Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced inflammation in human retinal endothelial cells |
| title_sort | epigallocatechin 3 gallate attenuates lipopolysaccharide induced inflammation in human retinal endothelial cells |
| topic | epigallocatechin-3-gallate human retinal endothelial cells inflammatory factors inflammatory factors |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067650/ |
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