Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin
Abstract Objective Salmonella spp. are one of the leading foodborne pathogens worldwide naturally found in the intestines of many animals. People that are in direct contact with the infected animals or their cages may become ill. The aim of this study was to determine the prevalence, antibiogram and...
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BMC
2019-06-01
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| Series: | BMC Research Notes |
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| Online Access: | http://link.springer.com/article/10.1186/s13104-019-4341-x |
| _version_ | 1828943760399007744 |
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| author | Esther Deguenon Victorien Dougnon Evelyne Lozes Nana Maman Jerrold Agbankpe Roula M. Abdel-Massih Fidélia Djegui Lamine Baba-Moussa Jacques Dougnon |
| author_facet | Esther Deguenon Victorien Dougnon Evelyne Lozes Nana Maman Jerrold Agbankpe Roula M. Abdel-Massih Fidélia Djegui Lamine Baba-Moussa Jacques Dougnon |
| author_sort | Esther Deguenon |
| collection | DOAJ |
| description | Abstract Objective Salmonella spp. are one of the leading foodborne pathogens worldwide naturally found in the intestines of many animals. People that are in direct contact with the infected animals or their cages may become ill. The aim of this study was to determine the prevalence, antibiogram and virulence genes associated with Salmonella serovars from fecal samples of animals intended for consumption in Southern Benin. Results Out of a total of 406 samples, 2.46% were positive. The isolates identified were multidrug-resistant Salmonella spp. to penicillins, first generation cephalosporins and some aminoglycosides. All Salmonella isolates produced invA gene of 284 bp, fimA of 85 bp and stn of 260 bp. The spvC gene (571 bp) was present in 10% of the isolates whereas the spvR gene (310 bp) was found in 20% of the isolates. The control strain possessed all the tested genes. The invA gene implies that strains are able to invade epithelial cells. The fimA and stn genes present in all isolates show that they are capable of causing gastrointestinal illness in humans. The presence of spvC and spvR genes suggests the possibility of these strains to produce toxins. |
| first_indexed | 2024-12-14T04:21:01Z |
| format | Article |
| id | doaj.art-ad98eaf0c83d4288ac44d5a6908bf67a |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-14T04:21:01Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-ad98eaf0c83d4288ac44d5a6908bf67a2022-12-21T23:17:22ZengBMCBMC Research Notes1756-05002019-06-011211710.1186/s13104-019-4341-xResistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in BeninEsther Deguenon0Victorien Dougnon1Evelyne Lozes2Nana Maman3Jerrold Agbankpe4Roula M. Abdel-Massih5Fidélia Djegui6Lamine Baba-Moussa7Jacques Dougnon8Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-CalaviResearch Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-CalaviResearch Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-CalaviResearch Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-CalaviResearch Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-CalaviDepartment of Biology, Faculty of Arts and Sciences, University of BalamandLaboratory of Veterinary Diagnosis and Serosurveillance of Parakou, Ministry of Agriculture, Livestock and FisheriesLaboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, UACResearch Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-CalaviAbstract Objective Salmonella spp. are one of the leading foodborne pathogens worldwide naturally found in the intestines of many animals. People that are in direct contact with the infected animals or their cages may become ill. The aim of this study was to determine the prevalence, antibiogram and virulence genes associated with Salmonella serovars from fecal samples of animals intended for consumption in Southern Benin. Results Out of a total of 406 samples, 2.46% were positive. The isolates identified were multidrug-resistant Salmonella spp. to penicillins, first generation cephalosporins and some aminoglycosides. All Salmonella isolates produced invA gene of 284 bp, fimA of 85 bp and stn of 260 bp. The spvC gene (571 bp) was present in 10% of the isolates whereas the spvR gene (310 bp) was found in 20% of the isolates. The control strain possessed all the tested genes. The invA gene implies that strains are able to invade epithelial cells. The fimA and stn genes present in all isolates show that they are capable of causing gastrointestinal illness in humans. The presence of spvC and spvR genes suggests the possibility of these strains to produce toxins.http://link.springer.com/article/10.1186/s13104-019-4341-xSalmonellaVirulence genesMultidrug resistance |
| spellingShingle | Esther Deguenon Victorien Dougnon Evelyne Lozes Nana Maman Jerrold Agbankpe Roula M. Abdel-Massih Fidélia Djegui Lamine Baba-Moussa Jacques Dougnon Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin BMC Research Notes Salmonella Virulence genes Multidrug resistance |
| title | Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin |
| title_full | Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin |
| title_fullStr | Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin |
| title_full_unstemmed | Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin |
| title_short | Resistance and virulence determinants of faecal Salmonella spp. isolated from slaughter animals in Benin |
| title_sort | resistance and virulence determinants of faecal salmonella spp isolated from slaughter animals in benin |
| topic | Salmonella Virulence genes Multidrug resistance |
| url | http://link.springer.com/article/10.1186/s13104-019-4341-x |
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