Excess of leptin inhibits hypothalamic expression in pubertal mice
PurposeLeptin has been considered a link between metabolic state and reproductive activity. Defective reproductive function can occur in leptin-deficient and leptin-excessive conditions. The aim of this study was to examine the effects of centrally injected leptin on the hypothalamic KiSS-1 system i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Pediatric Society
2012-09-01
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| Series: | Korean Journal of Pediatrics |
| Subjects: | |
| Online Access: | http://kjp.or.kr/upload/pdf/kjped-55-337.pdf |
| _version_ | 1818505464178016256 |
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| author | Sung Yeon Ahn Sei Won Yang Hee Jae Lee Jong Seon Byun Ji Yeon Om Choong Ho Shin |
| author_facet | Sung Yeon Ahn Sei Won Yang Hee Jae Lee Jong Seon Byun Ji Yeon Om Choong Ho Shin |
| author_sort | Sung Yeon Ahn |
| collection | DOAJ |
| description | PurposeLeptin has been considered a link between metabolic state and reproductive activity. Defective reproductive function can occur in leptin-deficient and leptin-excessive conditions. The aim of this study was to examine the effects of centrally injected leptin on the hypothalamic KiSS-1 system in relation to gonadotropin-releasing hormone (GnRH) action in the initial stage of puberty.MethodsLeptin (1 µg) was injected directly into the ventricle of pubertal female mice. The resultant gene expressions of hypothalamic GnRH and KiSS-1 and pituitary LH, 2 and 4 hours after injection, were compared with those of saline-injected control mice. The changes in the gene expressions after blocking the GnRH action were also analyzed.ResultsThe basal expression levels of KiSS-1, GnRH, and LH were significantly higher in the pubertal mice than in the prepubertal mice. The 1-µg leptin dose significantly decreased the mRNA expression levels of KiSS-1, GnRH, and LH in the pubertal mice. A GnRH antagonist significantly increased the KiSS-1 and GnRH mRNA expression levels, and the additional leptin injection decreased the gene expression levels compared with those in the control group.ConclusionThe excess leptin might have suppressed the central reproductive axis in the pubertal mice by inhibiting the KiSS-1 expression, and this mechanism is independent of the GnRH-LH-estradiol feedback loop. |
| first_indexed | 2024-12-10T21:51:08Z |
| format | Article |
| id | doaj.art-ad9cd2911136434b8ac1eee1a73bd560 |
| institution | Directory Open Access Journal |
| issn | 1738-1061 2092-7258 |
| language | English |
| last_indexed | 2024-12-10T21:51:08Z |
| publishDate | 2012-09-01 |
| publisher | Korean Pediatric Society |
| record_format | Article |
| series | Korean Journal of Pediatrics |
| spelling | doaj.art-ad9cd2911136434b8ac1eee1a73bd5602022-12-22T01:32:11ZengKorean Pediatric SocietyKorean Journal of Pediatrics1738-10612092-72582012-09-0155933734310.3345/kjp.2012.55.9.3372012600039Excess of leptin inhibits hypothalamic expression in pubertal miceSung Yeon Ahn0Sei Won Yang1Hee Jae Lee2Jong Seon Byun3Ji Yeon Om4Choong Ho Shin5Department of Pediatrics, Kangwon National University School of Medicine, Chuncheon, Korea.Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea.Department of Pharmacology, Kangwon National University School of Medicine, Chuncheon, Korea.Department of Pharmacology, Kangwon National University School of Medicine, Chuncheon, Korea.Department of Pharmacology, Kangwon National University School of Medicine, Chuncheon, Korea.Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea.PurposeLeptin has been considered a link between metabolic state and reproductive activity. Defective reproductive function can occur in leptin-deficient and leptin-excessive conditions. The aim of this study was to examine the effects of centrally injected leptin on the hypothalamic KiSS-1 system in relation to gonadotropin-releasing hormone (GnRH) action in the initial stage of puberty.MethodsLeptin (1 µg) was injected directly into the ventricle of pubertal female mice. The resultant gene expressions of hypothalamic GnRH and KiSS-1 and pituitary LH, 2 and 4 hours after injection, were compared with those of saline-injected control mice. The changes in the gene expressions after blocking the GnRH action were also analyzed.ResultsThe basal expression levels of KiSS-1, GnRH, and LH were significantly higher in the pubertal mice than in the prepubertal mice. The 1-µg leptin dose significantly decreased the mRNA expression levels of KiSS-1, GnRH, and LH in the pubertal mice. A GnRH antagonist significantly increased the KiSS-1 and GnRH mRNA expression levels, and the additional leptin injection decreased the gene expression levels compared with those in the control group.ConclusionThe excess leptin might have suppressed the central reproductive axis in the pubertal mice by inhibiting the KiSS-1 expression, and this mechanism is independent of the GnRH-LH-estradiol feedback loop.http://kjp.or.kr/upload/pdf/kjped-55-337.pdfLeptinKiSS-1Gonadotropin-releasing hormoneLuteinizing hormonePuberty |
| spellingShingle | Sung Yeon Ahn Sei Won Yang Hee Jae Lee Jong Seon Byun Ji Yeon Om Choong Ho Shin Excess of leptin inhibits hypothalamic expression in pubertal mice Korean Journal of Pediatrics Leptin KiSS-1 Gonadotropin-releasing hormone Luteinizing hormone Puberty |
| title | Excess of leptin inhibits hypothalamic expression in pubertal mice |
| title_full | Excess of leptin inhibits hypothalamic expression in pubertal mice |
| title_fullStr | Excess of leptin inhibits hypothalamic expression in pubertal mice |
| title_full_unstemmed | Excess of leptin inhibits hypothalamic expression in pubertal mice |
| title_short | Excess of leptin inhibits hypothalamic expression in pubertal mice |
| title_sort | excess of leptin inhibits hypothalamic expression in pubertal mice |
| topic | Leptin KiSS-1 Gonadotropin-releasing hormone Luteinizing hormone Puberty |
| url | http://kjp.or.kr/upload/pdf/kjped-55-337.pdf |
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