Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour.
Objective: To study the phytochemical and biological properties (antioxidant, anthelmintic and thrombolytic) of methanolic extracts of Enhydra fluctuans Lour., a plant belonging to the Asteraceae family. Methods: The phytochemical evaluation was carried out by qualitative analysis. In vitro antioxid...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-04-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115302100 |
| _version_ | 1818034838197764096 |
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| author | Sourov Kuri Md. Mustahsan Billah S.M. Masud Rana Zannatul Naim Md. Mahmodul Islam Md. Hasanuzzaman Md. Ramjan Ali Rana Banik |
| author_facet | Sourov Kuri Md. Mustahsan Billah S.M. Masud Rana Zannatul Naim Md. Mahmodul Islam Md. Hasanuzzaman Md. Ramjan Ali Rana Banik |
| author_sort | Sourov Kuri |
| collection | DOAJ |
| description | Objective: To study the phytochemical and biological properties (antioxidant, anthelmintic and thrombolytic) of methanolic extracts of Enhydra fluctuans Lour., a plant belonging to the Asteraceae family.
Methods: The phytochemical evaluation was carried out by qualitative analysis. In vitro antioxidant activity of extract was studied using free radical scavenging assay, ability of reduction, total phenol and total flavonoid contents determination assays. The anthelmintic activity was determined using paralysis and death time of Pheretima posthuma (earthworm) and thrombolytic activity by clot disruption assay.
Results: The phytochemical evaluation showed significant presence of flavonoids, triterpenes, carbohydrate, reducing sugars, saponins, phenols, diterpenes, protein and tannin. The antioxidant activity was found significant [IC50=(135.20±0.56) μg/mL] as compared to ascorbic acid [(130.00±0.76) µg/mL]. The reducing power was increased with concentration. Total phenol and total flavonoid contents were (153.08±0.38) mg/mL and (172.04±0.56) mg/mL respectively. The paralysis and death time of earthworms for different concentrations of extract were determined and compared with albendazole. The results showed that 10 mg/mL of the crude extract had similar effect with albendazole. Additionally, the crude extract showed a concentration depended relationship with its anthelmintic property. The clot lysis activity of crude extract was compared to the standard streptokinase's clot lysis (40.13%) activity and found significant (31%).
Conclusions: The study proves that the crude methanolic extract of Enhydra fluctuans Lour. has significant antioxidant, anthelmintic and thrombolytic activity containing wide range of phytochemicals. |
| first_indexed | 2024-12-10T06:45:31Z |
| format | Article |
| id | doaj.art-ad9cee22abeb4dc68db88fabcf57456a |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-10T06:45:31Z |
| publishDate | 2014-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-ad9cee22abeb4dc68db88fabcf57456a2022-12-22T01:58:41ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912014-04-014429930510.12980/APJTB.4.2014C677Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour.Sourov Kuri0Md. Mustahsan Billah1S.M. Masud Rana2Zannatul Naim3Md. Mahmodul Islam4Md. Hasanuzzaman5Md. Ramjan Ali6Rana Banik7Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, BangladeshDepartment of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, BangladeshDepartment of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, BangladeshObjective: To study the phytochemical and biological properties (antioxidant, anthelmintic and thrombolytic) of methanolic extracts of Enhydra fluctuans Lour., a plant belonging to the Asteraceae family. Methods: The phytochemical evaluation was carried out by qualitative analysis. In vitro antioxidant activity of extract was studied using free radical scavenging assay, ability of reduction, total phenol and total flavonoid contents determination assays. The anthelmintic activity was determined using paralysis and death time of Pheretima posthuma (earthworm) and thrombolytic activity by clot disruption assay. Results: The phytochemical evaluation showed significant presence of flavonoids, triterpenes, carbohydrate, reducing sugars, saponins, phenols, diterpenes, protein and tannin. The antioxidant activity was found significant [IC50=(135.20±0.56) μg/mL] as compared to ascorbic acid [(130.00±0.76) µg/mL]. The reducing power was increased with concentration. Total phenol and total flavonoid contents were (153.08±0.38) mg/mL and (172.04±0.56) mg/mL respectively. The paralysis and death time of earthworms for different concentrations of extract were determined and compared with albendazole. The results showed that 10 mg/mL of the crude extract had similar effect with albendazole. Additionally, the crude extract showed a concentration depended relationship with its anthelmintic property. The clot lysis activity of crude extract was compared to the standard streptokinase's clot lysis (40.13%) activity and found significant (31%). Conclusions: The study proves that the crude methanolic extract of Enhydra fluctuans Lour. has significant antioxidant, anthelmintic and thrombolytic activity containing wide range of phytochemicals.http://www.sciencedirect.com/science/article/pii/S2221169115302100Enhydra fluctuans Lour.PhytochemicalsAntioxidantDPPH radical scavengingPheretima posthumaStreptokinase |
| spellingShingle | Sourov Kuri Md. Mustahsan Billah S.M. Masud Rana Zannatul Naim Md. Mahmodul Islam Md. Hasanuzzaman Md. Ramjan Ali Rana Banik Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. Asian Pacific Journal of Tropical Biomedicine Enhydra fluctuans Lour. Phytochemicals Antioxidant DPPH radical scavenging Pheretima posthuma Streptokinase |
| title | Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
| title_full | Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
| title_fullStr | Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
| title_full_unstemmed | Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
| title_short | Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour. |
| title_sort | phytochemical and in vitro biological investigations of methanolic extracts of enhydra fluctuans lour |
| topic | Enhydra fluctuans Lour. Phytochemicals Antioxidant DPPH radical scavenging Pheretima posthuma Streptokinase |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115302100 |
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