Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)

Both in nature and in aquaculture, fish may experience periods of food scarcity or hunger. The metabolic regulation of fish when nutritional state changes is a complex process that involves many factors. To study glucose metabolism adaptability during fasting and re-feeding in the black carp (Myloph...

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Main Authors: Yafan Dai, Yubang Shen, Jiamin Guo, Hao Yang, Feng Chen, Wei Zhang, Wenhui Wu, Xiaoyan Xu, Jiale Li
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-03-01
Series:Aquaculture and Fisheries
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2468550X22000727
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author Yafan Dai
Yubang Shen
Jiamin Guo
Hao Yang
Feng Chen
Wei Zhang
Wenhui Wu
Xiaoyan Xu
Jiale Li
author_facet Yafan Dai
Yubang Shen
Jiamin Guo
Hao Yang
Feng Chen
Wei Zhang
Wenhui Wu
Xiaoyan Xu
Jiale Li
author_sort Yafan Dai
collection DOAJ
description Both in nature and in aquaculture, fish may experience periods of food scarcity or hunger. The metabolic regulation of fish when nutritional state changes is a complex process that involves many factors. To study glucose metabolism adaptability during fasting and re-feeding in the black carp (Mylopharyngodon piceus), we measured changes in some biochemical indicators related to glucose metabolism. Five fish were sampled on days 0, 1, 3, 5, and 10 of fasting (F, S1, S3, S5, and S10, respectively) and days 1, 3, and 5 of re-feeding (RF1, RF3, and RF5, respectively). The serum glucose concentration decreased significantly at S1, reached the lowest point at S10, and increased significantly at RF1 (P < 0.05). The concentration of liver glycogen decreased significantly at S1 and reached the lowest level at S3, whereas the muscle glycogen level decreased significantly at S5 and reached the lowest value at S10 (P < 0.05). Both liver and muscle glycogen levels returned to the pre-fasting level at RF5 (P < 0.05). Regarding glycolysis, the concentrations of pyruvate kinase (PK) and hexokinase (HK) decreased significantly at S5 and increased significantly at RF5 and RF1, respectively (P < 0.05). The concentrations of glucokinase (GCK) and insulin decreased significantly at S1 and increased significantly at RF1 and RF3, respectively (P < 0.05). The mRNA expression levels of liver GCK and glucose transporter 2 (GLUT2) decreased significantly at S1 and increased significantly at RF1 and RF5, respectively (P < 0.05). As for gluconeogenesis, the concentration of glucose-6-phosphatase (G6PC) increased significantly at S1 and decreased significantly at RF1 (P < 0.05). The concentrations of glucagon and glucocorticoid (GC) increased significantly at S3 and significantly decreased at RF1 and RF5, respectively (P < 0.05). The mRNA expression levels of liver G6PC and phosphoenolpyruvate carboxykinase (PEPCK) increased significantly at S3 and S1, and both decreased significantly at RF1 (P < 0.05). These results indicate that coordination between glycolysis and gluconeogenesis might be crucial for glucose homeostasis during fasting and re-feeding in the black carp.
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spelling doaj.art-ad9d3e58fccc43649c527761975bb8752024-02-11T05:11:46ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2024-03-0192226233Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)Yafan Dai0Yubang Shen1Jiamin Guo2Hao Yang3Feng Chen4Wei Zhang5Wenhui Wu6Xiaoyan Xu7Jiale Li8Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China; Corresponding author. College of Aquaculture and Life science, Shanghai Ocean University, Shanghai, 201306, China.Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, ChinaKey Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China; Corresponding author. College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China.Both in nature and in aquaculture, fish may experience periods of food scarcity or hunger. The metabolic regulation of fish when nutritional state changes is a complex process that involves many factors. To study glucose metabolism adaptability during fasting and re-feeding in the black carp (Mylopharyngodon piceus), we measured changes in some biochemical indicators related to glucose metabolism. Five fish were sampled on days 0, 1, 3, 5, and 10 of fasting (F, S1, S3, S5, and S10, respectively) and days 1, 3, and 5 of re-feeding (RF1, RF3, and RF5, respectively). The serum glucose concentration decreased significantly at S1, reached the lowest point at S10, and increased significantly at RF1 (P < 0.05). The concentration of liver glycogen decreased significantly at S1 and reached the lowest level at S3, whereas the muscle glycogen level decreased significantly at S5 and reached the lowest value at S10 (P < 0.05). Both liver and muscle glycogen levels returned to the pre-fasting level at RF5 (P < 0.05). Regarding glycolysis, the concentrations of pyruvate kinase (PK) and hexokinase (HK) decreased significantly at S5 and increased significantly at RF5 and RF1, respectively (P < 0.05). The concentrations of glucokinase (GCK) and insulin decreased significantly at S1 and increased significantly at RF1 and RF3, respectively (P < 0.05). The mRNA expression levels of liver GCK and glucose transporter 2 (GLUT2) decreased significantly at S1 and increased significantly at RF1 and RF5, respectively (P < 0.05). As for gluconeogenesis, the concentration of glucose-6-phosphatase (G6PC) increased significantly at S1 and decreased significantly at RF1 (P < 0.05). The concentrations of glucagon and glucocorticoid (GC) increased significantly at S3 and significantly decreased at RF1 and RF5, respectively (P < 0.05). The mRNA expression levels of liver G6PC and phosphoenolpyruvate carboxykinase (PEPCK) increased significantly at S3 and S1, and both decreased significantly at RF1 (P < 0.05). These results indicate that coordination between glycolysis and gluconeogenesis might be crucial for glucose homeostasis during fasting and re-feeding in the black carp.http://www.sciencedirect.com/science/article/pii/S2468550X22000727Mylopharyngodon piceusFastingGlucose metabolismGlycolysisGluconeogenesis
spellingShingle Yafan Dai
Yubang Shen
Jiamin Guo
Hao Yang
Feng Chen
Wei Zhang
Wenhui Wu
Xiaoyan Xu
Jiale Li
Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)
Aquaculture and Fisheries
Mylopharyngodon piceus
Fasting
Glucose metabolism
Glycolysis
Gluconeogenesis
title Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)
title_full Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)
title_fullStr Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)
title_full_unstemmed Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)
title_short Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)
title_sort glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re feeding in black carp mylopharyngodon piceus
topic Mylopharyngodon piceus
Fasting
Glucose metabolism
Glycolysis
Gluconeogenesis
url http://www.sciencedirect.com/science/article/pii/S2468550X22000727
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