Recent studies of synthetic antibody-based 3-MCPD determination technology
3-Chloro-1,2-propanediol (3-MCPD) is classified by the International Agency for Research on Cancer as carcinogenic material. 3-MCPD will also become one of the European Union's requirements, proposing the maximum level of the 3-MCPD in palm oil until 2.5 ppm. Although the reported technologies...
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| Format: | Article |
| Language: | English |
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indonesian research institute for biotechnology and bioindustry
2021-04-01
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| Series: | Menara Perkebunan |
| Subjects: | |
| Online Access: | http://mp.iribb.org/index.php/mpjurnal/article/view/402 |
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| author | Hasim Munawar Prima Luna Irma Kresnawaty Happy Widiastuti |
| author_facet | Hasim Munawar Prima Luna Irma Kresnawaty Happy Widiastuti |
| author_sort | Hasim Munawar |
| collection | DOAJ |
| description | 3-Chloro-1,2-propanediol (3-MCPD) is classified by the International Agency for Research on Cancer as carcinogenic material. 3-MCPD will also become one of the European Union's requirements, proposing the maximum level of the 3-MCPD in palm oil until 2.5 ppm. Although the reported technologies GCMS and HPLC-FLD demonstrated high sensitivity and selectivity on 3-MCPD determination, those technologies invest in chemical and time-consuming sample preparation and analysis. Molecularly imprinted polymer (MIP), or a synthetic antibody, can be used to recognize 3-MCPD. MIP is more robust under extreme environments such as temperature and pH. This paper, therefore, aims to discuss the application of MIP on sample extraction and analysis to detect 3-MCPD. MIP is synthesized by polymerization of functional monomers surrounding 3-MCPD as a template. 3-MCPD is then removed from the MIP, leaving active cavities. Thus, these sites can either covalently or non-covalently rebind to 3-MCPD. Computational or empirical studies could investigate the composition of MIP. MIP can be manufactured as MIP-based solid phase extraction (MIPSPE) and MIP-based sensor. Both applications showed significant analytical parameters, such as recovery higher than 90% and detection limit lower than 2.5 ppm. Therefore, the application of MIP can be flexible for sample preparation and analysis on the 3-MCPD determination. MIP-based technology would be a prospective instrument to detect 3-MCPD. In the future, producing MIP on an industrial scale will be a challenge to monitor the 3-MCPD level in palm oil. |
| first_indexed | 2024-12-16T15:00:22Z |
| format | Article |
| id | doaj.art-ada29c120c64495da94ead563be325b7 |
| institution | Directory Open Access Journal |
| issn | 0125-9318 1858-3768 |
| language | English |
| last_indexed | 2024-12-16T15:00:22Z |
| publishDate | 2021-04-01 |
| publisher | indonesian research institute for biotechnology and bioindustry |
| record_format | Article |
| series | Menara Perkebunan |
| spelling | doaj.art-ada29c120c64495da94ead563be325b72022-12-21T22:27:18Zengindonesian research institute for biotechnology and bioindustryMenara Perkebunan0125-93181858-37682021-04-01891627210.22302/iribb.jur.mp.v89i1.402Recent studies of synthetic antibody-based 3-MCPD determination technologyHasim Munawar0Prima Luna1Irma Kresnawaty 2Happy Widiastuti 3Balai Besar Penelitian VeterinerBalai Besar Litbang Pasca Panen PertanianPusat Penelitian Bioteknologi dan Bioindustri IndonesiaPusat Penelitian Bioteknologi dan Bioindustri Indonesia3-Chloro-1,2-propanediol (3-MCPD) is classified by the International Agency for Research on Cancer as carcinogenic material. 3-MCPD will also become one of the European Union's requirements, proposing the maximum level of the 3-MCPD in palm oil until 2.5 ppm. Although the reported technologies GCMS and HPLC-FLD demonstrated high sensitivity and selectivity on 3-MCPD determination, those technologies invest in chemical and time-consuming sample preparation and analysis. Molecularly imprinted polymer (MIP), or a synthetic antibody, can be used to recognize 3-MCPD. MIP is more robust under extreme environments such as temperature and pH. This paper, therefore, aims to discuss the application of MIP on sample extraction and analysis to detect 3-MCPD. MIP is synthesized by polymerization of functional monomers surrounding 3-MCPD as a template. 3-MCPD is then removed from the MIP, leaving active cavities. Thus, these sites can either covalently or non-covalently rebind to 3-MCPD. Computational or empirical studies could investigate the composition of MIP. MIP can be manufactured as MIP-based solid phase extraction (MIPSPE) and MIP-based sensor. Both applications showed significant analytical parameters, such as recovery higher than 90% and detection limit lower than 2.5 ppm. Therefore, the application of MIP can be flexible for sample preparation and analysis on the 3-MCPD determination. MIP-based technology would be a prospective instrument to detect 3-MCPD. In the future, producing MIP on an industrial scale will be a challenge to monitor the 3-MCPD level in palm oil.http://mp.iribb.org/index.php/mpjurnal/article/view/4023-mcpdmolecularly imprinted polymer (mip)mip-based spemip-based sensor |
| spellingShingle | Hasim Munawar Prima Luna Irma Kresnawaty Happy Widiastuti Recent studies of synthetic antibody-based 3-MCPD determination technology Menara Perkebunan 3-mcpd molecularly imprinted polymer (mip) mip-based spe mip-based sensor |
| title | Recent studies of synthetic antibody-based 3-MCPD determination technology |
| title_full | Recent studies of synthetic antibody-based 3-MCPD determination technology |
| title_fullStr | Recent studies of synthetic antibody-based 3-MCPD determination technology |
| title_full_unstemmed | Recent studies of synthetic antibody-based 3-MCPD determination technology |
| title_short | Recent studies of synthetic antibody-based 3-MCPD determination technology |
| title_sort | recent studies of synthetic antibody based 3 mcpd determination technology |
| topic | 3-mcpd molecularly imprinted polymer (mip) mip-based spe mip-based sensor |
| url | http://mp.iribb.org/index.php/mpjurnal/article/view/402 |
| work_keys_str_mv | AT hasimmunawar recentstudiesofsyntheticantibodybased3mcpddeterminationtechnology AT primaluna recentstudiesofsyntheticantibodybased3mcpddeterminationtechnology AT irmakresnawaty recentstudiesofsyntheticantibodybased3mcpddeterminationtechnology AT happywidiastuti recentstudiesofsyntheticantibodybased3mcpddeterminationtechnology |