Functional Characterization of Tea Plant (<i>Camellia sinensis</i> L.) <i>CsCBF2</i> Gene Involved in Multiple Abiotic Stress Response in Tobacco (<i>Nicotiana</i> <i>tabacum</i> L.)
C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (<i>Camellia sinensis</i> L.)...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/9/853 |
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| author | Qiying Zhou Dongxiao Liu Yiwan Wei Ning Ma Ruijiao Zhang Zengya Zhang Changjun Jiang Hongyu Yuan |
| author_facet | Qiying Zhou Dongxiao Liu Yiwan Wei Ning Ma Ruijiao Zhang Zengya Zhang Changjun Jiang Hongyu Yuan |
| author_sort | Qiying Zhou |
| collection | DOAJ |
| description | C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (<i>Camellia sinensis</i> L.) <i>CBF</i> gene (<i>CsCBF</i>) was still seldom reported. Here, functional study of the cold-responsive <i>CBF</i> gene (<i>CsCBF2</i>) was done. Results showed that CsCBF2 had conserved AP2 DNA-binding domain and the typical PKK/RPAGRxKFxETRHP and DSAWR signature sequences of CBF/DREB1. Yeast one-hybrid and transcription activation assays revealed that the activation domain of CsCBF2 could activate the reporter gene expression, and the N terminal of CsCBF2 displayed an inhibitory effect. Although CsCBF2 was conserved to bind the C-repeat/dehydration-responsive element (CRT/DRE), intact CsCBF2 protein preferred the CRT <i>cis</i> element. Under normal growth conditions, <i>CsCBF2</i>-overexpressing tobacco plants (<i>CsCBF2</i>-OX) exhibited lighter green leaf color, growth retardation, and dwarfism. Smaller leaf of <i>CsCBF2</i>-OX was only seen in eight weeks after been sown in soil. Under cold, salinity, or drought stress, <i>CsCBF2</i>-OX displayed better growing with longer roots, heavier fresh weight, higher germination rate, and accumulated more proline and sugar contents, but lower electrolyte leakage. The results demonstrated that <i>CsCBF2</i> enhanced plant tolerance to multiple abiotic stresses. |
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| id | doaj.art-ada4506230d0472e89cd99315d72988e |
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| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T23:50:55Z |
| publishDate | 2022-09-01 |
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| series | Horticulturae |
| spelling | doaj.art-ada4506230d0472e89cd99315d72988e2023-11-23T16:34:44ZengMDPI AGHorticulturae2311-75242022-09-018985310.3390/horticulturae8090853Functional Characterization of Tea Plant (<i>Camellia sinensis</i> L.) <i>CsCBF2</i> Gene Involved in Multiple Abiotic Stress Response in Tobacco (<i>Nicotiana</i> <i>tabacum</i> L.)Qiying Zhou0Dongxiao Liu1Yiwan Wei2Ning Ma3Ruijiao Zhang4Zengya Zhang5Changjun Jiang6Hongyu Yuan7Henan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang 464000, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang 464000, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang 464000, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang 464000, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang 464000, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang 464000, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang 464000, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang 464000, ChinaC-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (<i>Camellia sinensis</i> L.) <i>CBF</i> gene (<i>CsCBF</i>) was still seldom reported. Here, functional study of the cold-responsive <i>CBF</i> gene (<i>CsCBF2</i>) was done. Results showed that CsCBF2 had conserved AP2 DNA-binding domain and the typical PKK/RPAGRxKFxETRHP and DSAWR signature sequences of CBF/DREB1. Yeast one-hybrid and transcription activation assays revealed that the activation domain of CsCBF2 could activate the reporter gene expression, and the N terminal of CsCBF2 displayed an inhibitory effect. Although CsCBF2 was conserved to bind the C-repeat/dehydration-responsive element (CRT/DRE), intact CsCBF2 protein preferred the CRT <i>cis</i> element. Under normal growth conditions, <i>CsCBF2</i>-overexpressing tobacco plants (<i>CsCBF2</i>-OX) exhibited lighter green leaf color, growth retardation, and dwarfism. Smaller leaf of <i>CsCBF2</i>-OX was only seen in eight weeks after been sown in soil. Under cold, salinity, or drought stress, <i>CsCBF2</i>-OX displayed better growing with longer roots, heavier fresh weight, higher germination rate, and accumulated more proline and sugar contents, but lower electrolyte leakage. The results demonstrated that <i>CsCBF2</i> enhanced plant tolerance to multiple abiotic stresses.https://www.mdpi.com/2311-7524/8/9/853tea plantCsCBF2functional characterizationabiotic stressresistance |
| spellingShingle | Qiying Zhou Dongxiao Liu Yiwan Wei Ning Ma Ruijiao Zhang Zengya Zhang Changjun Jiang Hongyu Yuan Functional Characterization of Tea Plant (<i>Camellia sinensis</i> L.) <i>CsCBF2</i> Gene Involved in Multiple Abiotic Stress Response in Tobacco (<i>Nicotiana</i> <i>tabacum</i> L.) Horticulturae tea plant CsCBF2 functional characterization abiotic stress resistance |
| title | Functional Characterization of Tea Plant (<i>Camellia sinensis</i> L.) <i>CsCBF2</i> Gene Involved in Multiple Abiotic Stress Response in Tobacco (<i>Nicotiana</i> <i>tabacum</i> L.) |
| title_full | Functional Characterization of Tea Plant (<i>Camellia sinensis</i> L.) <i>CsCBF2</i> Gene Involved in Multiple Abiotic Stress Response in Tobacco (<i>Nicotiana</i> <i>tabacum</i> L.) |
| title_fullStr | Functional Characterization of Tea Plant (<i>Camellia sinensis</i> L.) <i>CsCBF2</i> Gene Involved in Multiple Abiotic Stress Response in Tobacco (<i>Nicotiana</i> <i>tabacum</i> L.) |
| title_full_unstemmed | Functional Characterization of Tea Plant (<i>Camellia sinensis</i> L.) <i>CsCBF2</i> Gene Involved in Multiple Abiotic Stress Response in Tobacco (<i>Nicotiana</i> <i>tabacum</i> L.) |
| title_short | Functional Characterization of Tea Plant (<i>Camellia sinensis</i> L.) <i>CsCBF2</i> Gene Involved in Multiple Abiotic Stress Response in Tobacco (<i>Nicotiana</i> <i>tabacum</i> L.) |
| title_sort | functional characterization of tea plant i camellia sinensis i l i cscbf2 i gene involved in multiple abiotic stress response in tobacco i nicotiana i i tabacum i l |
| topic | tea plant CsCBF2 functional characterization abiotic stress resistance |
| url | https://www.mdpi.com/2311-7524/8/9/853 |
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