Fluorometric Method to Assess Lipase Inhibition Activity
Obesity and excess weight have become serious health problems in our developed societies today. Increased blood pressure, blood glucose levels and abnormal blood lipids are frequent consequences. Inhibition of digestive enzymes by pharmacological or nutritional intervention are one avenue...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | deu |
| Published: |
Swiss Chemical Society
2009-12-01
|
| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://www.chimia.ch/chimia/article/view/4785 |
| _version_ | 1819278696721154048 |
|---|---|
| author | Wilfried Andlauer Patrice Prunier Denis Prim |
| author_facet | Wilfried Andlauer Patrice Prunier Denis Prim |
| author_sort | Wilfried Andlauer |
| collection | DOAJ |
| description |
Obesity and excess weight have become serious health problems in our developed societies today. Increased blood pressure, blood glucose levels and abnormal blood lipids are frequent consequences. Inhibition of digestive enzymes by pharmacological or nutritional intervention are one
avenue to be considered to treat this population. In the present study a robust assay to screen biologically active materials for their ability to inhibit pancreatic lipase, the most important enzyme in fat digestion, has been developed. Methyl-umbelliferyl butyrate was used as an artificial
substrate, enabling assessment of lipase activity via specific fluorescence emission. Applicability of the assay was shown by assessment of lipase inhibition activity of wild plants from Switzerland and France. Testing showed some plants to have a high inhibition rate of about 70%.
In further projects, this lipase inhibition assay could be used for a scientific proof of biological activity of raw materials with the intention to develop functional foods for weight reduction.
|
| first_indexed | 2024-12-24T00:16:07Z |
| format | Article |
| id | doaj.art-ada51d79244c471ba1532fbcdf4f4d19 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-12-24T00:16:07Z |
| publishDate | 2009-12-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-ada51d79244c471ba1532fbcdf4f4d192022-12-21T17:24:44ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242009-12-01631210.2533/chimia.2009.897Fluorometric Method to Assess Lipase Inhibition ActivityWilfried AndlauerPatrice PrunierDenis Prim Obesity and excess weight have become serious health problems in our developed societies today. Increased blood pressure, blood glucose levels and abnormal blood lipids are frequent consequences. Inhibition of digestive enzymes by pharmacological or nutritional intervention are one avenue to be considered to treat this population. In the present study a robust assay to screen biologically active materials for their ability to inhibit pancreatic lipase, the most important enzyme in fat digestion, has been developed. Methyl-umbelliferyl butyrate was used as an artificial substrate, enabling assessment of lipase activity via specific fluorescence emission. Applicability of the assay was shown by assessment of lipase inhibition activity of wild plants from Switzerland and France. Testing showed some plants to have a high inhibition rate of about 70%. In further projects, this lipase inhibition assay could be used for a scientific proof of biological activity of raw materials with the intention to develop functional foods for weight reduction. https://www.chimia.ch/chimia/article/view/4785DigestionFluorometric assayLipase inhibitionObesity |
| spellingShingle | Wilfried Andlauer Patrice Prunier Denis Prim Fluorometric Method to Assess Lipase Inhibition Activity CHIMIA Digestion Fluorometric assay Lipase inhibition Obesity |
| title | Fluorometric Method to Assess Lipase Inhibition Activity |
| title_full | Fluorometric Method to Assess Lipase Inhibition Activity |
| title_fullStr | Fluorometric Method to Assess Lipase Inhibition Activity |
| title_full_unstemmed | Fluorometric Method to Assess Lipase Inhibition Activity |
| title_short | Fluorometric Method to Assess Lipase Inhibition Activity |
| title_sort | fluorometric method to assess lipase inhibition activity |
| topic | Digestion Fluorometric assay Lipase inhibition Obesity |
| url | https://www.chimia.ch/chimia/article/view/4785 |
| work_keys_str_mv | AT wilfriedandlauer fluorometricmethodtoassesslipaseinhibitionactivity AT patriceprunier fluorometricmethodtoassesslipaseinhibitionactivity AT denisprim fluorometricmethodtoassesslipaseinhibitionactivity |