Reprogrammed <i>Pteropus</i> Bat Stem Cells as A Model to Study Host-Pathogen Interaction during <i>Henipavirus</i> Infection
Bats are natural hosts for numerous zoonotic viruses, including henipaviruses, which are highly pathogenic for humans, livestock, and other mammals but do not induce clinical disease in bats. <i>Pteropus</i> bats are identified as a reservoir of henipaviruses and the source of transmissi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/12/2567 |
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| author | Noémie Aurine Camille Baquerre Maria Gaudino Christian Jean Claire Dumont Sylvie Rival-Gervier Clémence Kress Branka Horvat Bertrand Pain |
| author_facet | Noémie Aurine Camille Baquerre Maria Gaudino Christian Jean Claire Dumont Sylvie Rival-Gervier Clémence Kress Branka Horvat Bertrand Pain |
| author_sort | Noémie Aurine |
| collection | DOAJ |
| description | Bats are natural hosts for numerous zoonotic viruses, including henipaviruses, which are highly pathogenic for humans, livestock, and other mammals but do not induce clinical disease in bats. <i>Pteropus</i> bats are identified as a reservoir of henipaviruses and the source of transmission of the infection to humans over the past 20 years. A better understanding of the molecular and cellular mechanisms allowing bats to control viral infections requires the development of relevant, stable, and permissive cellular experimental models. By applying a somatic reprogramming protocol to <i>Pteropus</i> bat primary cells, using a combination of ESRRB (Estrogen Related Receptor Beta), CDX2 (Caudal type Homeobox 2), and c-MYC (MYC proto-oncogene) transcription factors, we generated bat reprogrammed cells. These cells exhibit stem cell-like characteristics and neural stem cell molecular signature. In contrast to primary fibroblastic cells, these reprogrammed stem cells are highly permissive to henipaviruses and exhibit specific transcriptomic profiles with the particular expression of certain susceptibility factors such as interferon-stimulated genes (ISG), which may be related to viral infection. These <i>Pteropus</i> bat reprogrammed stem cells should represent an important experimental tool to decipher interactions during henipaviruses infection in <i>Pteropus</i> bats, facilitate isolation and production of bat-borne viruses, and to better understand the bat biology. |
| first_indexed | 2024-03-10T03:31:31Z |
| format | Article |
| id | doaj.art-ada5712543a24478b7a576056d3d8032 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:31:31Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ada5712543a24478b7a576056d3d80322023-11-23T09:39:55ZengMDPI AGMicroorganisms2076-26072021-12-01912256710.3390/microorganisms9122567Reprogrammed <i>Pteropus</i> Bat Stem Cells as A Model to Study Host-Pathogen Interaction during <i>Henipavirus</i> InfectionNoémie Aurine0Camille Baquerre1Maria Gaudino2Christian Jean3Claire Dumont4Sylvie Rival-Gervier5Clémence Kress6Branka Horvat7Bertrand Pain8Stem Cell and Brain Research Institute, University of Lyon, Université Lyon 1, INSERM, INRAE, U1208, USC1361, 69500 Bron, FranceStem Cell and Brain Research Institute, University of Lyon, Université Lyon 1, INSERM, INRAE, U1208, USC1361, 69500 Bron, FranceInternational Center for Infectiology Research (CIRI), University of Lyon, Université Claude Bernard Lyon 1, INSERM, U1111, CNRS, UMR5308, ENS Lyon, 69007 Lyon, FranceStem Cell and Brain Research Institute, University of Lyon, Université Lyon 1, INSERM, INRAE, U1208, USC1361, 69500 Bron, FranceInternational Center for Infectiology Research (CIRI), University of Lyon, Université Claude Bernard Lyon 1, INSERM, U1111, CNRS, UMR5308, ENS Lyon, 69007 Lyon, FranceStem Cell and Brain Research Institute, University of Lyon, Université Lyon 1, INSERM, INRAE, U1208, USC1361, 69500 Bron, FranceStem Cell and Brain Research Institute, University of Lyon, Université Lyon 1, INSERM, INRAE, U1208, USC1361, 69500 Bron, FranceInternational Center for Infectiology Research (CIRI), University of Lyon, Université Claude Bernard Lyon 1, INSERM, U1111, CNRS, UMR5308, ENS Lyon, 69007 Lyon, FranceStem Cell and Brain Research Institute, University of Lyon, Université Lyon 1, INSERM, INRAE, U1208, USC1361, 69500 Bron, FranceBats are natural hosts for numerous zoonotic viruses, including henipaviruses, which are highly pathogenic for humans, livestock, and other mammals but do not induce clinical disease in bats. <i>Pteropus</i> bats are identified as a reservoir of henipaviruses and the source of transmission of the infection to humans over the past 20 years. A better understanding of the molecular and cellular mechanisms allowing bats to control viral infections requires the development of relevant, stable, and permissive cellular experimental models. By applying a somatic reprogramming protocol to <i>Pteropus</i> bat primary cells, using a combination of ESRRB (Estrogen Related Receptor Beta), CDX2 (Caudal type Homeobox 2), and c-MYC (MYC proto-oncogene) transcription factors, we generated bat reprogrammed cells. These cells exhibit stem cell-like characteristics and neural stem cell molecular signature. In contrast to primary fibroblastic cells, these reprogrammed stem cells are highly permissive to henipaviruses and exhibit specific transcriptomic profiles with the particular expression of certain susceptibility factors such as interferon-stimulated genes (ISG), which may be related to viral infection. These <i>Pteropus</i> bat reprogrammed stem cells should represent an important experimental tool to decipher interactions during henipaviruses infection in <i>Pteropus</i> bats, facilitate isolation and production of bat-borne viruses, and to better understand the bat biology.https://www.mdpi.com/2076-2607/9/12/2567batsstem cellsreprogrammingemerging infection<i>Henipavirus</i>Nipah virus |
| spellingShingle | Noémie Aurine Camille Baquerre Maria Gaudino Christian Jean Claire Dumont Sylvie Rival-Gervier Clémence Kress Branka Horvat Bertrand Pain Reprogrammed <i>Pteropus</i> Bat Stem Cells as A Model to Study Host-Pathogen Interaction during <i>Henipavirus</i> Infection Microorganisms bats stem cells reprogramming emerging infection <i>Henipavirus</i> Nipah virus |
| title | Reprogrammed <i>Pteropus</i> Bat Stem Cells as A Model to Study Host-Pathogen Interaction during <i>Henipavirus</i> Infection |
| title_full | Reprogrammed <i>Pteropus</i> Bat Stem Cells as A Model to Study Host-Pathogen Interaction during <i>Henipavirus</i> Infection |
| title_fullStr | Reprogrammed <i>Pteropus</i> Bat Stem Cells as A Model to Study Host-Pathogen Interaction during <i>Henipavirus</i> Infection |
| title_full_unstemmed | Reprogrammed <i>Pteropus</i> Bat Stem Cells as A Model to Study Host-Pathogen Interaction during <i>Henipavirus</i> Infection |
| title_short | Reprogrammed <i>Pteropus</i> Bat Stem Cells as A Model to Study Host-Pathogen Interaction during <i>Henipavirus</i> Infection |
| title_sort | reprogrammed i pteropus i bat stem cells as a model to study host pathogen interaction during i henipavirus i infection |
| topic | bats stem cells reprogramming emerging infection <i>Henipavirus</i> Nipah virus |
| url | https://www.mdpi.com/2076-2607/9/12/2567 |
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